Secoiridoid glycosides as a marker system in chemical variability estimation and chemotype assignment of Centaurium erythraea Rafn from the Balkan Peninsula

Šiler, Branislav; Avramov, Stevan; Banjanac, Tijana; Cvetković, Jelena Petković; Živković, Jelena; Patenković, Aleksandra; Mišić, Danijela

doi:10.1016/j.indcrop.2012.03.026

Cited by 22 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sweroside (4), swertiamarin (5), and gentiopicrin (6) are reported as the most abundant secoiridoids from this family, and it is presumed that the biosynthetic pathway leading from 4 to 5 and 6 is universally present in the whole Gentianaceae family [36]. In some species, roots are the site of the highest SGs accumulation [37,38], while, in others, including C. erythraea, these compounds are more abundant in the above-ground parts [4,[39][40][41]. Furthermore, the content of total SGs in C. erythraea leaves is reported not to be significantly influenced by the developmental stage [4].…”

Section: Resultsmentioning

confidence: 99%

Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves

Božunović

Škorić

Matekalo

et al. 2019

Plants

Self Cite

View full text Add to dashboard Cite

Centaurium erythraea Rafn produces and accumulates various biologically active specialized metabolites, including secoiridoid glucosides (SGs), which help plants to cope with unfavorable environmental conditions. Specialized metabolism is commonly modulated in a way to increase the level of protective metabolites, such as SGs. Here, we report the molecular background of the wounding-induced changes in SGs metabolism for the first time. The mechanical wounding of leaves leads to a coordinated up-regulation of SGs biosynthetic genes and corresponding JA-related transcription factors (TFs) after 24 h, which results in the increase of metabolic flux through the biosynthetic pathway and, finally, leads to the elevated accumulation of SGs 96 h upon injury. The most pronounced increase in relative expression was detected for secologanin synthase (CeSLS), highlighting this enzyme as an important point for the regulation of biosynthetic flux through the SG pathway. A similar expression pattern was observed for CeBIS1, imposing itself as the TF that is prominently involved in wound-induced regulation of SGs biosynthesis genes. The high degree of positive correlations between and among the biosynthetic genes and targeted TFs expressions indicate the transcriptional regulation of SGs biosynthesis in response to wounding with a significant role of CeBIS1, which is a known component of the jasmonic acid (JA) signaling pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves

Božunović

Škorić

Matekalo

et al. 2019

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…14,15 Earlier studies have also reported that gentiopicricin and swertiamarin are dominant compounds present in roots of Centaurium erythraea, a Gentianaceae plant. 24 Extracts of in vitro grown E. littorale showed potent antioxidant and AChEi capacities. Since cholinergic deficit is one of the most early detected symptoms in neurodegenerative diseases like Alzheimer's and Parkinson's diseases and thus, inhibition of AChE is a viable therapeutic strategy.…”

Section: Discussionmentioning

confidence: 98%

“…Quantitative AChEi activity of E. littorale was studied using Ellman's method. 24 The IC 50 value (mg/ml) was calculated, and Duncan's multiple range test was performed after ANOVA (P < 0.05)( Table 2). Eserine salicylate was used as the standard inhibitor of AChE (IC 50 = 0.0013 ± 0.02).…”

Section: Achei Studiesmentioning

confidence: 99%

Evaluation of antioxidant capacity and acetylcholinesterase inhibitory activity of field-grown plants and in vitro plantlets of Enicostemma littorale

et al. 2014

View full text Add to dashboard Cite

Background: Enicostemma littorale is an ethno-medicinally important plant belonging to Gentianaceae family. Objective: The main aim of this study was to establish tissue culture of E. littorale and compare the antioxidant capacities and acetylcholinesterase inhibitory (AChEi) properties of in vitro and field-grown E. littorale plants. Materials and Methods: Antioxidant capacities for various E. littorale extracts were studied in terms of 1,1-diphenyl-2picrylhydrazyl and 2,2′-azinobis-3 ethylbenzothiazoline-6-sulfonic acid free-radical scavenging along with ferric reducing antioxidant power and total phenolic content was also determined. AChEi properties were also compared between fieldgrown and in vitro E. littorale extracts. Results: In vitro plantlets of E. littorale was established in Murashige and Skoog (MS) medium with three different hormonal combinations, viz. naphthalene acetic acid (1 mg/l) and 6-benzylaminopurine (BAP)(0.2 mg/l) or N 1 B 0.2 , BAP (1 mg/l) and kinetin (0.1 mg/l) or B 1 K 0.1 and indole butyric acid (IBA) (1 mg/l) or IBA 1. Alcoholic extracts of shoots developed in MS medium supplemented with B 1 K 0.1 showed highest antioxidant potential than other extracts. Alcoholic extracts of plantlets grown in MS medium supplemented with IBA 1 showed both antioxidant and AChEi potential equivalently to field-grown extracts. Conclusion: In vitro plantlets grown in MS medium half strength supplemented with IBA 1 can be considered as most suitable for rapid proliferation because it offers advantage of equivalent potential of bioactivity in terms of antioxidant capacity and AChEi when compared to field-grown plants. Further studies can lead to better utilization of therapeutic potential of E. littorale.

show abstract

“…Quantitative analysis of bioactivity compounds (such as gentiopicroside, sweroside, swertiamarin, isoorientin, and other compounds) from rhizomes, stems, leaves, and flowers indicated that northwest of Yunnan-Guizhou Plateau was suitable for chemical compounds accumulation [13,14,15,16]. Additionally, conversion and transport of those compounds might be influenced by climatic conditions in the plant habitat [14,17].…”

Section: Introductionmentioning

confidence: 99%

“…yunnanensis , Macrohyporia cocos , and other species indicated that reliable classification results were also available by the fusion analysis of chemical fingerprint data collected from different medicinal parts of herbs [35,49]. Accumulation and distribution of metabolites in the different parts of plants were different because of the differential response of root, stem, flower and other organs to the environment variation of producing area [17,50]. Therefore, fingerprint data fusion of multi-medicinal parts may provide integrated chemical information for the authentication of medicinal materials.…”

Section: Introductionmentioning

confidence: 99%

Assessing Geographical Origin of Gentiana Rigescens Using Untargeted Chromatographic Fingerprint, Data Fusion and Chemometrics

Shen

Wang

2019

Molecules

View full text Add to dashboard Cite

Gentiana rigescens Franchet, which is famous for its bitter properties, is a traditional drug of chronic hepatitis and important raw materials for the pharmaceutical industry in China. In the study, high-performance liquid chromatography (HPLC), coupled with diode array detector (DAD) and chemometrics, were used to investigate the chemical geographical variation of G. rigescens and to classify medicinal materials, according to their grown latitudes. The chromatographic fingerprints of 280 individuals and 840 samples from rhizomes, stems, and leaves of four different latitude areas were recorded and analyzed for tracing the geographical origin of medicinal materials. At first, HPLC fingerprints of underground and aerial parts were generated while using reversed-phase liquid chromatography. After the preliminary data exploration, two supervised pattern recognition techniques, random forest (RF) and orthogonal partial least-squares discriminant analysis (OPLS-DA), were applied to the three HPLC fingerprint data sets of rhizomes, stems, and leaves, respectively. Furthermore, fingerprint data sets of aerial and underground parts were separately processed and joined while using two data fusion strategies (“low-level” and “mid-level”). The results showed that classification models that are based OPLS-DA were more efficient than RF models. The classification models using low-level data fusion method built showed considerably good recognition and prediction abilities (the accuracy is higher than 99% and sensibility, specificity, Matthews correlation coefficient, and efficiency range from 0.95 to 1.00). Low-level data fusion strategy combined with OPLS-DA could provide the best discrimination result. In summary, this study explored the latitude variation of phytochemical of G. rigescens and developed a reliable and accurate identification method for G. rigescens that were grown at different latitudes based on untargeted HPLC fingerprint, data fusion, and chemometrics. The study results are meaningful for authentication and the quality control of Chinese medicinal materials.

show abstract

Secoiridoid glycosides as a marker system in chemical variability estimation and chemotype assignment of Centaurium erythraea Rafn from the Balkan Peninsula

Cited by 22 publications

References 46 publications

Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves

Secoiridoids Metabolism Response to Wounding in Common Centaury (Centaurium erythraea Rafn) Leaves

Evaluation of antioxidant capacity and acetylcholinesterase inhibitory activity of field-grown plants and in vitro plantlets of Enicostemma littorale

Assessing Geographical Origin of Gentiana Rigescens Using Untargeted Chromatographic Fingerprint, Data Fusion and Chemometrics

Contact Info

Product

Resources

About