Sec-Page Characterization of Lake Aquatic Humic Matter Isolated with Xad-Resin and Tangential Membrane Ultrafiltration

Peuravuori, Juhani; Pihlaja, Kalevi; Trubetskaya, O. E.; Reznikova, O. I.; Afanas'eva, G. V.; Trubetskoj, Oleg

doi:10.1080/03067310108044379

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…8,10,11,19,20,23,27,30 For the separation of aquatic and terrestrial humic substances (HS) we have developed a combination of preparative LPSEC and analytical polyacrylamide gel electrophoresis (PAGE), called LPSEC-PAGE, 12 which was for the first time applied to aquatic NOM extracted from rivers and lakes of the U.S. and Finland. 33,34 Protein-like fluorescence was found in the highest and medium molecular size fractions, while humic-like fluorescence was detected mostly in the low molecular size fraction. The latter fraction also showed the highest capacity to sensitize the formation of singlet oxygen.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Conventional low pressure size-exclusion chromatography (LPSEC) − and high-performance size-exclusion liquid chromatography (HPSEC) ,− are often used to characterize aquatic and terrestrial NOM due to their high fractionation efficiency. To improve information provided by SEC, researchers frequently employed several techniques (UV–visible absorption, carbon content, 2D- and 3D-fluorescence, 1 H- and 13 C NMR, FT-ICR-MS, Pyr-GC-MS) hyphenated in a single analytical chromatographic run ,, or conducted offline. ,,,,,,, For the separation of aquatic and terrestrial humic substances (HS) we have developed a combination of preparative LPSEC and analytical polyacrylamide gel electrophoresis (PAGE), called LPSEC-PAGE, which was for the first time applied to aquatic NOM extracted from rivers and lakes of the U.S. and Finland. , Protein-like fluorescence was found in the highest and medium molecular size fractions, while humic-like fluorescence was detected mostly in the low molecular size fraction. The latter fraction also showed the highest capacity to sensitize the formation of singlet oxygen .…”

Section: Introductionmentioning

confidence: 99%

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Molecular Size Distribution of Fluorophores in Aquatic Natural Organic Matter: Application of HPSEC with Multi-Wavelength Absorption and Fluorescence Detection Following LPSEC-PAGE Fractionation

Trubetskoj

Richard

Voyard

et al. 2018

Environ. Sci. Technol.

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Analytical high performance size exclusion liquid chromatography (HPSEC) with multiwavelength absorbance and fluorescence detections was used for the analysis of molecular size distribution and optical properties of dissolved natural organic matter. Experiments were conducted on Suwannee River organic matter (SRNOM) and its fractions A, B, C+D preliminary obtained by combination of preparative low pressure size exclusion chromatography and polyacrylamide gel electrophoresis (LPSEC-PAGE) and purified by dialysis on membrane with nominal cutoff 10 kDa, the fractions molecular size varied in order A > B > C + D > 10 kDa. The multistep fractionation of SRNOM enabled the size-separation of at least five types of humic-like fluorophores within NOM showing emission maxima at 465, 450, 435, 420, and 405 nm. The decrease of the humic-like emission maxima paralleled the decrease of the nominal molecular size of fluorescent SRNOM. The protein-like fluorescence was split into tyrosine-like and tryptophan-like fluorophores and only detected in fractions A and B. This work provides new data on the optical properties of size-fractionated NOM, which consistent with the formation of supramolecular NOM assemblies, likely controlled by association of low-molecular size components. It is clearly observed for the high molecular size fraction A, containing free amino acids or short peptides. The combination of several different fractionation procedures is very useful for obtaining less complex NOM compounds and understanding the NOM function in the environment.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Size Distribution of Fluorophores in Aquatic Natural Organic Matter: Application of HPSEC with Multi-Wavelength Absorption and Fluorescence Detection Following LPSEC-PAGE Fractionation

Trubetskoj

Richard

Voyard

et al. 2018

Environ. Sci. Technol.

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Evaluation of Suwannee River NOM electrophoretic fractions by RP-HPLC with online absorbance and fluorescence detection

Trubetskaya

Richard

Trubetskoj

2015

Environ Sci Pollut Res

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Reversed-phase high-performance liquid chromatography (RP-HPLC) with online absorbance and fluorescence detections was used for the evaluation of Suwannee River natural organic matter (SRNOM) and its fractions A, B, and C+D, obtained by conventional size exclusion chromatography-polyacrylamide gel electrophoresis (SEC-PAGE) setup, for which the electrophoretic mobility (EM) and the absorptivity varied in the order C+D > B > A, and the molecular size (MS) in the opposite order. Analysis of SRNOM and its fractions in part of their relative irreversible adsorption on C18-column and relative distribution of eluted from the column matter on hydrophobic and hydrophilic peaks showed that hydrophobicity of fractions decreased in order: A > B > C+D. The online fluorescence detection showed that SRNOM and its fractions contained at least three groups of humic substances (HS)-like fluorophores with emission maxima at 435, 455-465, and 455/420 nm and two protein-like fluorophores with emission maxima at around 300 and 340 nm. The HS-like fluorophore with emission maximum at 435 nm was located in the hydrophilic peak in all the samples. Those with maxima at 455-465 nm were detected in hydrophobic peaks of fractions A and B. SEC-PAGE setup followed by RP-HPLC allowed us to develop new approach of SRNOM separation on less heterogeneous compounds mixture for their further study and structural identification.

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Photochemical activity and fluorescence of electrophoretic fractions of aquatic humic matter

2009

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Sec-Page Characterization of Lake Aquatic Humic Matter Isolated with Xad-Resin and Tangential Membrane Ultrafiltration

Cited by 3 publications

References 22 publications

Molecular Size Distribution of Fluorophores in Aquatic Natural Organic Matter: Application of HPSEC with Multi-Wavelength Absorption and Fluorescence Detection Following LPSEC-PAGE Fractionation

Molecular Size Distribution of Fluorophores in Aquatic Natural Organic Matter: Application of HPSEC with Multi-Wavelength Absorption and Fluorescence Detection Following LPSEC-PAGE Fractionation

Evaluation of Suwannee River NOM electrophoretic fractions by RP-HPLC with online absorbance and fluorescence detection

Photochemical activity and fluorescence of electrophoretic fractions of aquatic humic matter

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