Seaweed Proteins and Applications in Animal Feed

García‐Vaquero, Marco

doi:10.1002/9781119385332.ch7

Cited by 4 publications

(2 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macroalgae are a rich source of multiple biologically active compounds including polysaccharides and proteins [17,18]. Chlorophyta (green) and Rhodophyta (red) macroalgae are the most promising algae to be exploited as sources of protein for functional food and feed development [17,19]. Amongst the green macroalgae, Ulva sp.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Approaches to Generating and Identifying Angiotensin-Converting Enzyme I Inhibitory Peptides from Green Macroalga Ulva lactuca

2019

View full text Add to dashboard Cite

A protein extract was generated from the macroalga Ulva lactuca, which was subsequently hydrolysed using the food-grade enzyme papain and angiotensin-converting Enzyme I and renin inhibitory peptides identified using a combination of enrichment strategies employing molecular weight cutoff filtration and mass spectrometry analysis. The generated hydrolysates with the most promising in vitro activity were further purified using preparative RP-HPLC and characterised. The 1 kDa hydrolysate (1 kDa-UFH), purified and collected by preparative RP-HPLC at minutes 41‒44 (Fr41‒44), displayed statistically higher ACE-I inhibitory activities ranging from 96.91% to 98.06%. A total of 48 novel peptides were identified from these four fractions by LC-MS/MS. A simulated gastrointestinal digestion of the identified peptide sequences was carried out using in silico enzyme cleavage simulation tools, resulting in 86 peptide sequences that were further assessed for their potential activity, toxicity and allergenicity using multiple predictive approaches. All the peptides obtained in this study were predicted to be non-toxic. However, 28 out of the 86 novel peptides released after the in silico gastrointestinal digestion were identified as potential allergens. The potential allergenicity of these peptides should be further explored to comply with the current labelling regulations in formulated food products containing U. lactuca protein hydrolysates.

show abstract

Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Approaches to Generating and Identifying Angiotensin-Converting Enzyme I Inhibitory Peptides from Green Macroalga Ulva lactuca

2019

View full text Add to dashboard Cite

show abstract

“…In addition, is possible to use a single enzyme or two or more enzymes (Kim & Wijesekara 2010). Physicochemical conditions of enzymes in the process and hydrolysis conditions, such as temperature and pH, are important factors in the peptide composition and in the ACEi activity (García-Vaquero 2018).…”

Section: Discussionmentioning

confidence: 99%

Algae as a source of peptides inhibitors of the angiotensin-converting enzyme: a systematic review

Amorim

Silva

Costa

et al. 2022

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Hypertension is a factor that contributes to the risk of chronic diseases. The inhibition of angiotensin-I converting enzyme (ACE) is a useful therapeutic approach to the hypertension treatment. The algae have been an alternative for the production of ACE inhibitory (ACEi) peptides from enzymatic hydrolysis due to their protein-rich biomass. The aim of this study was to systematically review the literature regarding the production, composition and activity of ACEi peptides derived from algae proteins. Systematic database searches identifi ed 648 related articles. Among these, only 14 were selected according to the eligibility criteria to this review. Macroalgae are more studied than microalgae as sources of ACEi peptides. Furthermore, hydrolysates by thermolysin or bromelain exhibited the highest ACEi activity compared to other enzymes. The main features of the peptides with high ACE inhibition are low molecular weight, short amino acids sequence and non-competitive inhibition pattern. In vivo studies using hydrolysates and peptides derived from algae proteins showed antihypertensive activity in spontaneously hypertensive rats (SHR). Thus, it is suggested that ACEi peptides derived from algae can be considered as potential antihypertensive.

show abstract

Precipitation and characterization of Pacific dulse (Devaleraea mollis) proteins

Mendez

Kwon

2022

J Appl Phycol

View full text Add to dashboard Cite

Seaweed Proteins and Applications in Animal Feed

Cited by 4 publications

References 72 publications

In Vitro and In Silico Approaches to Generating and Identifying Angiotensin-Converting Enzyme I Inhibitory Peptides from Green Macroalga Ulva lactuca

In Vitro and In Silico Approaches to Generating and Identifying Angiotensin-Converting Enzyme I Inhibitory Peptides from Green Macroalga Ulva lactuca

Algae as a source of peptides inhibitors of the angiotensin-converting enzyme: a systematic review

Precipitation and characterization of Pacific dulse (Devaleraea mollis) proteins

Contact Info

Product

Resources

About