Seawater-acclimation abates cold effects on Na+, K+-ATPase activity in gills of the juvenile milkfish, Chanos chanos

Kang, Can; Chen, Yi Chi; Chang, Chia-Hao; Tsai, Shu-Chuan; Lee, Tsung‐Han

doi:10.1016/j.aquaculture.2015.04.022

Cited by 37 publications

(36 citation statements)

References 46 publications

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“…The expression of SDHA here revealed differential expression at 18°C between FW and SW milkfish. Since the gill is the major osmoregulatory organ, the unchanged expression of SDHA found in this study, together with the slightly elevated level in the gill of milkfish at 18°C [ 26 ], implied that hypothermia affected energy metabolism in milkfish but not osmotic homeostasis. In fish, one of the most important survival strategies for cold acclimation is an increase in the concentration of aerobic metabolic enzymes, such as the enzymes involved in the Krebs cycle and oxidative phosphorylation, which reside within the mitochondria [ 61 ].…”

Section: Discussionmentioning

confidence: 90%

“…Milkfish is a euryhaline teleost, which modified its physiology to adapt to environments of different salinities. However, osmoregulation is energy-consuming mechanisms and gill Na + , K + -ATPase activity decrease under low temperature [ 26 ]. Thus, systemic investigation on metabolic genes expression was provided to verify differences for low-temperature responses in seawater or freshwater milkfish.…”

Section: Discussionmentioning

confidence: 99%

“…Salinities affected the hypothermal tolerating ability of the milkfish. The critical thermal minimum for SW milkfish is 13°C compared to 15°C in FW milkfish [ 26 ]. Thus, gill, brain, liver, and kidney which are full of glycogen accumulation and highly metabolic organs [ 30 – 34 ] were sampled in this study.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Analysis of Metabolic Pathways in Milkfish That Respond to Salinity and Temperature Changes

Kang

Tang

et al. 2015

PLoS ONE

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Milkfish (Chanos chanos), an important marine aquaculture species in southern Taiwan, show considerable euryhalinity but have low tolerance to sudden drops in water temperatures in winter. Here, we used high throughput next-generation sequencing (NGS) to identify molecular and biological processes involved in the responses to environmental changes. Preliminary tests revealed that seawater (SW)-acclimated milkfish tolerated lower temperatures than the fresh water (FW)-acclimated group. Although FW- and SW-acclimated milkfish have different levels of tolerance for hypothermal stress, to date, the molecular physiological basis of this difference has not been elucidated. Here, we performed a next-generation sequence analysis of mRNAs from four groups of milkfish. We obtained 29669 unigenes with an average length of approximately 1936 base pairs. Gene ontology (GO) analysis was performed after gene annotation. A large number of genes for molecular regulation were identified through a transcriptomic comparison in a KEGG analysis. Basal metabolic pathways involved in hypothermal tolerance, such as glycolysis, fatty acid metabolism, amino acid catabolism and oxidative phosphorylation, were analyzed using PathVisio and Cytoscape software. Our results indicate that in response to hypothermal stress, genes for oxidative phosphorylation, e.g., succinate dehydrogenase, were more highly up-regulated in SW than FW fish. Moreover, SW and FW milkfish used different strategies when exposed to hypothermal stress: SW milkfish up-regulated oxidative phosphorylation and catabolism genes to produce more energy budget, whereas FW milkfish down-regulated genes related to basal metabolism to reduce energy loss.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Transcriptomic Analysis of Metabolic Pathways in Milkfish That Respond to Salinity and Temperature Changes

Kang

Tang

et al. 2015

PLoS ONE

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“…Being a marine euryhaline teleost inhabiting the tropical and subtropical zone of the Indo-Pacific Ocean (Bagarinao, 1994), milkfish are intolerant to temperatures lower than 12°C (Kang et al, 2015). Moreover, SW (seawater)-acclimated milkfish exhibited higher hypothermal tolerance than FW (fresh water)-acclimated milkfish to critical thermal minimum (CT Min ) analysis (Kang et al, 2015). Hsieh and Kuo (2005) reported that under cold stress (15°C) FW-acclimated milkfish would regulate desaturation of fatty acids to maintain membrane fluidity.…”

Section: Introductionmentioning

confidence: 99%

“…Under non-lethal low temperatures (18°C), gill Na + , K + -ATPase (NKA) activity of SW milkfish showed a partial compensatory response to moderate cold effects and maintained ion homeostasis. Furthermore, relative amounts of heat shock protein 70 in milkfish gills were increased to stabilize protein structure under temperature fluctuations (Kang et al, 2015). The proteomic analyses of milkfish livers under hypothermal stress detected a pI shift of two Prdx6 protein spots (Chang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge

Chang

Lee

2016

Front. Physiol.

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A tropical species, the euryhaline milkfish (Chanos chanos), is a crucial economic species in Southeast Asia and is intolerant of water temperature below 12°C. Large numbers of milkfish die during cold periods in winter. Hypothermal environments usually increase oxidative stress in teleosts, and the liver is the major organ for anti-oxidative responses in the body. Peroxiredoxin-6 (Prdx6) in mammals is a multi-functional enzyme and acts as both glutathione peroxidase, phospholipase A2 and acyl-transferase for maintenance of redox status and prevention of cell membrane peroxidation. Prdx6 can protect cells from oxidant-induced membrane damage by translocating the Prdx6 protein from the cytosol to the membrane. Upon cold stress, Ccprdx6 transcript levels were up-regulated after 24 h and 96 h in livers of fresh water (FW)- and seawater (SW)-acclimated milkfish, respectively. In the hypothermal FW group, the Prdx6 protein was up-regulated in the cytosol of hepatocytes with a similar role as glutathione peroxidase to reduce oxidative stress upon hypothermal challenge. Conversely, in hypothermal SW milkfish, total Prdx6 protein was down-regulated. However, cytosolic Prdx6 protein was translocated to the membrane, using the ability of phospholipase A2 to stabilize the membrane redox state. Moreover, H2O2 content was increased in FW-acclimated milkfish livers upon hypothermal challenge. Ex vivo H2O2 treatment of milkfish livers also induced Ccprdx6 transcriptional expression, which provided more evidence of the antioxidant role of milkfish Prdx6. Taken together, upon hypothermal challenge, greater oxidative stress in livers of FW-acclimated milkfish rather than SW-acclimated individuals led to different profiles of hepatic CcPrdx6 expression between the FW and SW group. The results indicated that CcPrdx6 played the role of antioxidant with different mechanisms, i.e., binding to reactive oxygen species and stabilizing membrane fluidity, in livers of hypothermal FW and SW milkfish, respectively.

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Aquaculture Fish Responses Towards Temperature Stress: A Critical Review

Naz

Iqbal

Khan

et al. 2023

Toxicology and Human Health

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Seawater-acclimation abates cold effects on Na+, K+-ATPase activity in gills of the juvenile milkfish, Chanos chanos

Cited by 37 publications

References 46 publications

Transcriptomic Analysis of Metabolic Pathways in Milkfish That Respond to Salinity and Temperature Changes

Transcriptomic Analysis of Metabolic Pathways in Milkfish That Respond to Salinity and Temperature Changes

The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge

Aquaculture Fish Responses Towards Temperature Stress: A Critical Review

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