Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest

Rasche, Frank; Knapp, Daniela; Kaiser, Christina; Koranda, Marianne; Kitzler, Barbara; Zechmeister‐Boltenstern, Sophie; Richter, Andreas; Sessitsch, Angela

doi:10.1038/ismej.2010.138

Cited by 290 publications

(216 citation statements)

References 75 publications

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“…Similar to previous studies, we found a significant influence of seasonality on soil microbial community composition (Kaiser et al 2010;Koranda et al 2013;Rasche et al 2011). From summer to winter, bacterial and fungal PLFA markers increased slightly.…”

Section: Soil Microbial Community Compositionsupporting

confidence: 78%

Contribution of litter layer to soil greenhouse gas emissions in a temperate beech forest

et al. 2016

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Background and aims The litter layer is a major source of CO 2 , and it also influences soil-atmosphere exchange of N 2 O and CH 4 . So far, it is not clear how much of soil greenhouse gas (GHG) emission derives from the litter layer itself or is litter-induced. The present study investigates how the litter layer controls soil GHG fluxes and microbial decomposer communities in a temperate beech forest. Methods We removed the litter layer in an Austrian beech forest and studied responses of soil CO 2 , CH 4 and N 2 O fluxes and the microbial community via phospholipid fatty acids (PLFA). Soil GHG fluxes were determined with static chambers on 22 occasions from July 2012 to February 2013, and soil samples collected at 8 sampling events.Results Litter removal reduced CO 2 emissions by 30 % and increased temperature sensitivity (Q 10 ) of CO 2 fluxes. Diffusion of CH 4 into soil was facilitated by litter removal and CH 4 uptake increased by 16 %. This effect was strongest in autumn and winter when soil moisture was high. Soils without litter turned from net N 2 O sources to slight N 2 O sinks because N 2 O emissions peaked after rain events in summer and autumn, which was not the case in litter-removal plots. Microbial composition was only transiently affected by litter removal but strongly influenced by seasonality. Conclusions Litter layers must be considered in calculating forest GHG budgets, and their influence on temperature sensitivity of soil GHG fluxes taken into account for future climate scenarios.

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Section: Soil Microbial Community Compositionsupporting

confidence: 78%

Contribution of litter layer to soil greenhouse gas emissions in a temperate beech forest

et al. 2016

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“…Temperature has a negative correlation with bacterial amoA gene copy numbers and a positive correlation with thaumarchaeal amoA gene copy numbers in a temperate beech forest soil (Rasche et al 2011). The AOA amoA gene copy numbers decreased along an altitudinal gradient of Mount Everest, whereas that of AOB did not shift significantly with altitude (Zhang et al 2009).…”

Section: Responses Of Aob and Aoa Abundance To Soil Warming And Fertimentioning

confidence: 97%

“…1b). The effects of temperature on the amoA gene copy numbers of AOB and AOA have been observed in seasonal or altitude changes (He et al 2007;Zhang et al 2009;Rasche et al 2011). For example, AOB and AOA amoA gene copy numbers have been found to be higher in summer than winter in a Chinese upland red soil (He et al 2007).…”

Section: Responses Of Aob and Aoa Abundance To Soil Warming And Fertimentioning

confidence: 99%

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

Long

Chen

et al. 2012

J Soils Sediments

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Purpose Boreal forests are considered to be more sensitive to global climate change compared with other terrestrial ecosystems, but the long-term impact of climate change and forest management on soil microbial functional diversity is not well understood. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are the most important players in nitrogen (N) cycling-associated processes in terrestrial ecosystems. This study investigated the separate and combined impacts of long-term soil warming and fertilization on soil AOB and AOA community structures and abundances in a Norway spruce stand in northern Sweden. Materials and methods The soil-warming experiment was established in the buffer zones of two irrigated plots (I) and complete nutrient solution plots (IL) since 1995. The warming treatment started in April each year by maintaining soil temperature on warmed plots at 5°C above the temperature in unwarmed plots using heating cables. In August 2006, soil samples were collected from eight subplots for molecular analysis. The abundance of bacterial and archaeal amoA genes was determined by quantitative polymerase chain reaction. Similarly, total bacterial and archaeal population sizes have also been determined. The diversity of AOB and AOA was assessed by constructing amoA gene clone libraries, and different genotypes were screened with restriction fragment length polymorphism. Results and discussion Results showed that fertilization did not significantly affect the abundance of the bacterial amoA gene under either warming or non-warming conditions; however, warming decreased the abundance under fertilization treatments. No significant effects of fertilization and soil warming were observed on the number of thaumarchaeal amoA gene copies across all treatments. In this study, amoA gene abundance of AOB was significantly higher than that of AOA across all treatments. The community structure of both AOB and AOA was strongly influenced by fertilization. For bacterial amoA genes, Nitrosospira cluster 2 was present across all treatments, but the only genotype was observed in the fertilization treatments while, for thaumarchaeal amoA genes, the relative abundance of soil cluster 5 increased in fertilization treatments. By comparison, soil-warming effects on AOB and AOA community structure were not significant. Canonical correspondence analysis showed a positive correlation between fertilization and both dominant genotypes of AOB and AOA. Conclusions These results indicated that the abundance of AOA and AOB was not affected by fertilization or warming alone, but the interaction of fertilization and warming reduced the abundance of AOB. The community composition of ammonia-oxidizers was more affected by the nutrientoptimized fertilization than the soil warming.

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“…Even within DNA-based methods, the differences in DNA extraction methods, DNA yield, the choice of primer sets, as well as qPCR efficiencies, limit the transferability of the results to other studied ecosystems [53]. Nevertheless, both nir genes are known to correlate with soil net N 2 O fluxes [33,[54][55][56]. nirS-possessing organisms usually are comprised of nosZ genes which enable complete denitrification, while nirK organisms mostly lack nosZ [57].…”

Section: The Varying Effects On Denitrification Genesmentioning

confidence: 99%

The Abundance of Fungi, Bacteria and Denitrification Genes during Insect Outbreaks in Scots Pine Forests

Grüning

Beule

Meyer

et al. 2018

Forests

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Abstract:Outbreaks of defoliating insects may affect microbial populations in forests and thereby mass balances and ecosystem functioning. Here, we investigated the microbial dynamics in Scots pine (Pinus sylvestris L.) forests during outbreaks of the nun moth (Lymantria monacha L.) and the pine-tree lappet (Dendrolimus pini L.). We used real-time PCR (polymerase chain reaction) to quantify genes that characterize bacterial and fungal abundance and the denitrification processes (nirK, nirS, nosZ clades I and II) in different forest compartments and we analyzed the C and N content of pine needles, insect feces, larvae, vegetation layers, organic layers, and mineral soil horizons. The infestation of the nun moth increased the bacterial abundance on pine needles, in the vegetation layer, and in the upper organic layer, while fungal populations were increased in the vegetation layer and upper organic layer during both outbreaks. In soil, the abundance of nirK increased after insect defoliation, while the C/N ratios decreased. nosZ clades I and II showed variable responses in different soil layers and to different defoliating insects. Our results illustrate changes in the microbial populations in pine forests that were infested by defoliating insects and changes in the chemical soil properties that foster these populations, indicating a genetic potential for increased soil N 2 O emissions during the defoliation peak of insect outbreak events.

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Seasonality and resource availability control bacterial and archaeal communities in soils of a temperate beech forest

Cited by 290 publications

References 75 publications

Contribution of litter layer to soil greenhouse gas emissions in a temperate beech forest

Contribution of litter layer to soil greenhouse gas emissions in a temperate beech forest

Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

The Abundance of Fungi, Bacteria and Denitrification Genes during Insect Outbreaks in Scots Pine Forests

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