Seasonality and chemical elicitation of defense oleoresin production in field-grown slash pine under subtropical climate

Rodrigues-Corrêa, Kelly Cristine da Silva; Fett-Neto, Arthur Germano

doi:10.1590/s2197-00252013000100007

Cited by 19 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although chemical stimulant pastes have been intensely studied in Pinus elliottii Engelm var. elliottii (slash pine) aiming at higher yields of oleoresin (Rodrigues et al, 2008 , 2011 ; Rodrigues and Fett-Neto, 2009 ; Rodrigues-Corrêa and Fett-Neto, 2013 ), knowledge of genes and their regulatory pattern for terpene biosynthesis in commercial forests are still unknown. Acquiring this information would be a crucial aspect to understand the biochemical and physiological basis of oleoresin production.…”

Section: Introductionmentioning

confidence: 99%

Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis

et al. 2016

View full text Add to dashboard Cite

Pine oleoresin is a major source of terpenes, consisting of turpentine (mono- and sesquiterpenes) and rosin (diterpenes) fractions. Higher oleoresin yields are of economic interest, since oleoresin derivatives make up a valuable source of materials for chemical industries. Oleoresin can be extracted from living trees, often by the bark streak method, in which bark removal is done periodically, followed by application of stimulant paste containing sulfuric acid and other chemicals on the freshly wounded exposed surface. To better understand the molecular basis of chemically-stimulated and wound induced oleoresin production, we evaluated the stability of 11 putative reference genes for the purpose of normalization in studying Pinus elliottii gene expression during oleoresinosis. Samples for RNA extraction were collected from field-grown adult trees under tapping operations using stimulant pastes with different compositions and at various time points after paste application. Statistical methods established by geNorm, NormFinder, and BestKeeper softwares were consistent in pointing as adequate reference genes HISTO3 and UBI. To confirm expression stability of the candidate reference genes, expression profiles of putative P. elliottii orthologs of resin biosynthesis-related genes encoding Pinus contorta β-pinene synthase [PcTPS-(−)β-pin1], P. contorta levopimaradiene/abietadiene synthase (PcLAS1), Pinus taeda α-pinene synthase [PtTPS-(+)αpin], and P. taeda α-farnesene synthase (PtαFS) were examined following stimulant paste application. Increased oleoresin yields observed in stimulated treatments using phytohormone-based pastes were consistent with higher expression of pinene synthases. Overall, the expression of all genes examined matched the expected profiles of oleoresin-related transcript changes reported for previously examined conifers.

show abstract

Section: Introductionmentioning

confidence: 99%

Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…How to reduce the cost of pastes and achieve a similar promoting effect is important for the popularization and application of resin-stimulant pastes. Previous studies indicated that it was possible to replace commercial resin-stimulant pastes containing CEPA with the pastes containing potassium ions in P. elliottii 9 , 16 . Our study showed that there was no significant difference in total resin yield between the K 2 SO 4 and CEPA treatment groups, while the resin yield of the treatment group with NAA or BA was lower than that of the CEPA treatment group in 2019.…”

Section: Discussionmentioning

confidence: 99%

“…Auxin is another molecule that can promote ethylene biosynthesis and resin canal differentiation. Metal cofactors of terpene synthases, such as iron or potassium ions, are also used as replacements for CEPA 16 . It is known that either auxin (2,4-dichlorophenoxyacetic acid, 2,4-D) or salicylic acid have a similar effect to CEPA on increasing resin production 15 .…”

Section: Introductionmentioning

confidence: 99%

“…It is known that either auxin (2,4-dichlorophenoxyacetic acid, 2,4-D) or salicylic acid have a similar effect to CEPA on increasing resin production 15 . In addition, it has been reported that the application of potassium (K) could lead to a higher resin yield than CEPA 9 , 16 . A systematic study compared the effect of auxin (naphthaleneacetic acid, NAA), BA, K and CEPA on resin yield 12 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Efficient resin production using stimulant pastes in Pinus elliottii × P. caribaea families

Liu

Wang

FenCheng

et al. 2022

Sci Rep

View full text Add to dashboard Cite

To address the increasing labor cost of resin tapping, more efficient methods for resin tapping need to be developed. This study aimed to evaluate the features of resinosis as affected by stimulant pastes in Pinus elliottii × P. caribaea, which is also one of the predominant resin-producing species hybrids in South China. The resin yields and resin compositions were assessed in 33 P. elliottii × P. caribaea F1 families, with the application of four kinds of chemical stimulants, potassium (K2SO4) paste, naphthalene acetic acid (NAA) paste, benzoic acid (BA) paste and 2-chloroethylphosphonic acid (CEPA) paste. Our results showed that all four pastes significantly increased the resin yield by at least 20% at each tapping, and 3- to fivefold increases were detected at the beginning of each year. The correlations between resin yield and growth at each tapping ranged from uncorrelated to moderately positively correlated, indicating that resin yield was mostly but not always determined by tree size. The concentration of each resin component did not change with the stimulant applications. In P. elliottii × P. caribaea, selecting a larger tree diameter at breast height and employing the chemical stimulants at the first several tapping rounds are efficient tapping procedures. Moreover, the K2SO4-based stimulant can be recommended considering its promoting effects on resin yield and the low cost of the chemicals required to produce it.

show abstract

“…var. elliottii revealed its seasonal variation [17]. The highest yield of oleoresin was observed from the tapped trees during summer.…”

Section: Introductionmentioning

confidence: 94%

Seasonal Changes in the Composition of Scots Pine (Pinus Syvestris L.) Oleoresin, Measured by the NMR Method

Skakovskii,

L.Yu.,

Gapan’kova

et al. 2023

Russ J Bioorg Chem

View full text Add to dashboard Cite

Seasonal changes in the composition of Scots pine oleoresin were analyzed by 1 H and 13 C NMR spectroscopy. Due to favorable weather conditions (positive temperatures on the sampling dates throughout the year) samples for each of the 12 months were obtained and studied. Chloroform (CDCl 3 ) solutions of oleoresin were investigated. Eight resin acids: abietic, dehydroabietic, isopimaric, levopimaric, neoabietic, palustric, pimaric, and sandaracopimaric acids, as well as six monoterpenes: camphene, limonene, myrcene, α-pinene, β-pinene, and terpinolene, were identified and quantified. It was revealed that the amounts of the oleoresin released and its constituent α-pinene decreased at low temperatures. Other monoterpenes were not detected within the measurement accuracy in this period of time. It was supposed that monoterpenes, formed in smaller amounts during the period of pests' anabiosis, play the key role in the control of coniferous insect pests. The contents of dehydroabietic, isopimaric, neoabietic, pimaric, and sandaracopimaric acids were found to vary insignificantly throughout the year. An interrelation between the amounts of abietic, levopimaric, and palustric acids in the oleoresin composition was revealed. The observed interrelation was supposedly associated with low-temperature catalytic reactions of levopimaric acid isomerization leading to predominant formation of abietic acid. It was suggested that these processes should be taken into account to avoid errors in determining the contents of these acids in the oleoresin even in the case of statistical analysis.

show abstract

Seasonality and chemical elicitation of defense oleoresin production in field-grown slash pine under subtropical climate

Cited by 19 publications

References 30 publications

Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis

Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis

Efficient resin production using stimulant pastes in Pinus elliottii × P. caribaea families

Seasonal Changes in the Composition of Scots Pine (Pinus Syvestris L.) Oleoresin, Measured by the NMR Method

Contact Info

Product

Resources

About