Seasonal variations in the survival index of rats at simulated high altitudes

Bhatia, B.; Thomas, Sharyn; Purkayastha, Sushmita

doi:10.1007/bf01636121

Cited by 14 publications

(7 citation statements)

References 3 publications

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“…According to the literature, this parameter characterizes the organism’s resistance to hypoxia 27–30. Adult male Wistar rats were exposed, one at a time, to simulated hypobaric hypoxia, equivalent to the altitude of 11,500 m, as described previously27–30,32,40–42 in an animal decompression chamber coupled to a mercury barometer (equivalent to 180 mmHg). All the decompressions and recompressions were achieved gradually at the rate of 600 m (≈40 mmHg)/min to prevent any tissue injury to the organism as a result of a sudden fall or rise in ambient pressure.…”

Section: Methodsmentioning

confidence: 99%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

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The aim of the study was to characterize the severity of the systemic inflammatory response induced by lipopolysaccharide (LPS) in animals with different resistance levels to hypoxia. Materials and methods: Two to three months old male Wistar rats (220-240 g) were divided according to hypoxia tolerance in a hypobaric chamber. After a month, they were injected intraperitoneally with Escherichia coli LPS at a dose of 1.5 mg/kg. After 3, 6 and 24 hours of LPS injection, we studied the levels of IL-1β, C-reactive protein (CRP) and TGF-β in the serum, the expression of Hif-1α and Nf-kb in the liver, morphological disorders in the lung and ex vivo production of IL-10 by splenic cells activated by ConA. Results: In the early periods after the injection of LPS, increase in Nf-kb expression in the liver was observed only in the rats susceptible to hypoxia. After 6 hours of LPS injection, the number of neutrophils in the interalveolar septa of the lungs of rats susceptible to hypoxia was higher than in tolerant rats. This points to the development of more pronounced LPS-induced inflammation in the rats susceptible to hypoxia and is accompanied by increased expression of Hif-1α in the liver after 6 hours of LPS administration, serum IL-1β level after 3 hours and CRP level after 24 hours. The production of the anti-inflammatory cytokine IL-10 by the spleen was significantly decreased after 6 hours of LPS injection only in the animals tolerant to hypoxia. After 24 hours of LPS injection, a significant decrease in serum TGF-β level occurred in the rats tolerant to hypoxia in comparison with the control group, which improved the survival rates of the animals. Conclusion: We have demonstrated the differences in the severity of the LPS-induced inflammatory response in male Wistar rats with different resistance levels to hypoxia. Rats susceptible to hypoxia are characterized by a more pronounced inflammatory response induced by LPS.

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Section: Methodsmentioning

confidence: 99%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

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“…Hypoxic tolerance was determined by measuring the gasping time (GT) and hypoxic survival time (HST) by exposing animals one at a time to simulated hypobaric hypoxia of 10,668 m in an animal decompression chamber at 32°C (decibel instruments) coupled to a mercury barometer (Bhatia et al, 1966). The airflow in the chamber was 2L/min, while the relative humidity was maintained at 40% to 50%.…”

Section: Cocl 2 Treatment and Determination Of Hypoxic Tolerancementioning

confidence: 99%

Cobalt Supplementation Promotes Hypoxic Tolerance and Facilitates Acclimatization to Hypobaric Hypoxia in Rat Brain

Shrivastava

Ram

Bansal

et al. 2008

High Altitude Medicine & Biology

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In the present study, we report the molecular mechanisms of action by cobalt in facilitating acclimatization to hypobaric hypoxia using male Sprague-Dawley rats as the model system. We determined hypoxic gasping time and survival time as a measure to assess the degree of tolerance of animals to hypobaric hypoxia by exposing the animals to an altitude of 10,668 m. Oral administration of cobalt chloride (12.5 mg Co/kg body weight, BW, for 7 days) increased gasping time and hypoxic survival time by 3 to 4 times compared to the control animals. This could be attributed to an increased expression and the DNA binding activity of hypoxia inducible transcriptional factor (HIF-1alpha) and its regulated genes, that is, erythropoietin (EPO), vascular endothelial growth factor (VEGF), glucose transporter-1 (Glut-1), and nitric oxide synthase (NOS) levels. This in turn leads to better oxygenation, oxygen delivery, glucose transport, and maintenance of vascular tone, respectively, under oxygen-limited conditions. This was further confirmed by lower levels of lactate dehydrogenase (LDH) activity and lactate in the brain of cobalt + hypoxia group compared with animals exposed to hypoxia. Glucose levels also increased after cobalt supplementation. The findings of the study provide a basis for the possible use of cobalt for facilitating acclimatization to hypoxia and other conditions involving oxygen deprivation.

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“…Hypoxic tolerance was determined by measuring the time taken for the onset of gasping (gasping time). Adult male Wistar rats were exposed, one at a time, to simulated hypobaric hypoxia, equivalent to the altitude of 11,500 m, as described previously [5–9, 11, 27–29] in an animal decompression chamber coupled to a mercury barometer (equivalent to 180 mmHg). All the decompression and recompression instances were achieved gradually at a rate of 600 m (≈40 mmHg)/min to prevent any tissue injury as a result of a sudden fall or rise in ambient pressure.…”

Section: Methodsmentioning

confidence: 99%

Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory Response in Male Rats with Different Resistance to Hypoxia

Джалилова

Косырева

Diatroptov

et al. 2019

International Journal of Inflammation

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On the model of the systemic inflammatory response (SIRS), induced by lipopolysaccharide (LPS), the morphological and functional changes in the thymus and spleen and the subpopulation composition of peripheral blood lymphocytes of rats differing in resistance to hypoxia were studied. It was demonstrated that the level of endotoxin in blood serum after 3 hours of LPS administration in susceptible-to-hypoxia rats was 64 times higher than in the control group, while in tolerant-to-hypoxia animals it was only 8 times higher in 6 hours. After 24 hours of LPS injection, only in susceptible-to-hypoxia rats did the level of C-reactive protein in blood serum increase. There is a difference in the dynamics of morphological changes of lymphoid organs after LPS injection in tolerant- and susceptible-to-hypoxia animals. After 3 hours of LPS administration, the tolerant-to-hypoxia rats showed no changes in the thymus, spleen, and subpopulation composition of lymphocytes in peripheral blood. After 6 hours there was only a decrease in B-lymphocytes and increase in cytotoxic T-lymphocytes and NK cells. After 1 day of LPS injection, the tolerant-to-hypoxia rats had devastation in PALS of the spleen. After 3 hours of LPS injection the susceptible-to-hypoxia animals had reactive changes in the lymphoid organs: decrease of the thymus cortex, narrowing of the marginal zones of spleen lymphoid follicles, widening of their germinal centers, and a decrease in the absolute number of cytotoxic T-lymphocytes, NK cells, and B-lymphocytes. After 24 hours of LPS injection the tolerant-to-hypoxia animals had a greater absolute number of T-lymphocytes and NK cells in comparison with the susceptible rats. Thus, in animals with different resistance to hypoxia the LPS-induced SIRS is characterized by different dynamics of morphological and functional changes of the thymus and spleen. The obtained data will serve as a basis for the development of new individual approaches to the prevention and treatment of infectious and inflammatory diseases.

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Seasonal variations in the survival index of rats at simulated high altitudes

Cited by 14 publications

References 3 publications

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Cobalt Supplementation Promotes Hypoxic Tolerance and Facilitates Acclimatization to Hypobaric Hypoxia in Rat Brain

Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory Response in Male Rats with Different Resistance to Hypoxia

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