Seasonal variations in the intermediate metabolism of the crayfish Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) in the natural environment and experimental culture

Dutra, Bibiana Kaiser; Zank, Caroline; Silva, Karina M. da; Conter, Maria R.; Oliveira, Guendalina Turcato

doi:10.1590/s0073-47212008000300010

Cited by 18 publications

(10 citation statements)

References 38 publications

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“…In decapod crustaceans, the maintenance of suitable glucose levels in the hemolymph is essential to support the regular functions of various organs and in responses to environmental stress (Verri et al, 2001;Lorenzon et al, 2005;Dutra et al, 2008). The digestion of glycogenic substrates and absorption of glucose via the hepatopancreas are the main sources of hemolymphatic glucose (Verri et al, 2001;Obi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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“…Subsequently, the levels of glucose, lactate, arginine phosphate, free glucose, glycogen, total protein, total lipids, and cholesterol were measured. The tests were performed by standard spectrophotometric methods as used for other crustaceans in the Laboratório de Fisiologia da Conservação, of the Pontifícia Universidade Católica do Rio Grande do Sul (Oliveira et al, 2003;Oliveira et al, 2007;Silva-Castiglioni et al, 2007;Buckup et al, 2008;Dutra et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Studies about intermediary metabolism were conducted in Brazil only with the crayfishes Parastacus varicosus, Parastacus defossus, and Parastacus brasiliensis by Silva-Castiglioni et al (2007), Buckup et al (2008), and Dutra et al (2008), respectively. These studies showed the importance of carbohydrates, total proteins, and fats in the life cycle of the animals, which were related mainly to the diet or reproductive period of the species, and showed seasonal variations in reserves.…”

Section: Introductionmentioning

confidence: 99%

“…Buckup et al (2008), studying the seasonal variations on the intermediary metabolism of P. defossus, verified that the lipids seem to be an important reserve of energy used during reproduction in both sexes, while glycogen may be used during periods of intense activity or fasting. Already, Dutra et al (2008) verified in P. brasiliensis that the environmental conditions (e.g., food availability and water temperature) and reproductive period appeared to be the main factors influencing the seasonal patterns of variation in energy metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the seasonal variation of the biochemical composition in two species of freshwater crayfish from southern Brazil

et al. 2012

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The aim of this study was to compare seasonal variations in the metabolism of two crayfish species with different habitats, Parastacus defossus that lives in subterranean galleries with low oxygen concentrations and Parastacus brasiliensis that lives in oxygenated environments. The metabolism of the species was compared, mainly evaluating the mobilization of the reserves for the reproductive period, and the metabolic response during the seasonal cycle. Seasonal sampling was conducted from spring 2006 to winter 2007. P. brasiliensis was collected at Mariana Pimentel, and P. defossus at Lami, Porto Alegre, Rio Grande do Sul. Hemolymph, hepatopancreas, muscle, gills, and gonads were removed for determination of glucose, free glucose, glycogen, lactate, total protein, total lipids, total cholesterol, and arginine phosphate. The hepatopancreatic and gonadosomatic indexes were determined. The results showed different seasonal responses between the species for all metabolic parameters, with the exception of proteins in gills, and of lactate in haemolymph. Lactate levels were slightly, but insignificantly, higher in P. defossus than in P. brasiliensis during the period of reduced oxygen. A significant difference between sexes was recorded in only some metabolites. The variability of the metabolism appears to be related to the reproductive period, but the exploratory activity in the environment also seems to be related to these variations. However, other factors may also be influencing the metabolism, such as the availability of food, and environmental conditions such as water, pH, temperature and dissolved oxygen levels, as previously observed for other decapods.

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“…Since the pioneer work of Kucharski and Da Silva ('91), which described the seasonal variation in the energy metabolism of the estuarine crab Neohelice granulata (formerly Chasmagnathus granulata), a number of studies have assessed the seasonal variation in the energy metabolism of crustacean species in Southern Brazil, including two aeglids (Oliveira et al, 2003, three parastacids (Silva-Castiglioni et al, 2007;Buckup et al, 2008;Dutra et al, 2008b), two amphipods (Dutra et al, , 2008a, and the ghost crab, O. quadrata (Vinagre et al, 2007). These studies revealed the existence of wide inter and intraspecific variability, which makes it difficult to determine a standard metabolic profile.…”

mentioning

confidence: 99%

Seasonal variations in the biochemical composition and reproductive cycle of the ghost crab Ocypode quadrata (Fabricius, 1787) in Southern Brazil

et al. 2010

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The ghost crab, Ocypode quadrata, is found on sandy beaches from the United States to Southern Brazil. Because there is still little information about the metabolism and reproduction of O. quadrata on the southern coast of Brazil, the objectives of this study were to (i) evaluate the effect of seasonal variations on the carbohydrate, lipid, and protein metabolism of O. quadrata at Rondinha Beach, a beach with high anthropogenic activity, and to compare it with data from Siriú Beach, which has less human activity; and (ii) describe the effect of seasonal variations on the histological characteristics of male and female gonads, in order to assess the reproductive capacity of the crabs. The gonads of male crabs showed no significant variations in the gonadosomatic index (GSI) and glycogen levels; however, histological analysis of the testes revealed that they are mature in the summer. In females, the GSI and glycogen values are higher in summer, concomitantly with the presence of mature oocytes. These results suggest that the reproductive peak of O. quadrata occurs in summer. The seasonal analysis of the biochemical parameters, as well as comparison with Siriú Beach, demonstrated that the ghost crabs of Rondinha Beach have a different pattern of metabolism than those of Siriú. This difference may be a consequence of differences in the environmental conditions as well as in the anthropogenic pressures, such as vehicle traffic and the increase in human population at the beach in summer.

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Seasonal variations in the intermediate metabolism of the crayfish Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) in the natural environment and experimental culture

Cited by 18 publications

References 38 publications

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Comparison of the seasonal variation of the biochemical composition in two species of freshwater crayfish from southern Brazil

Seasonal variations in the biochemical composition and reproductive cycle of the ghost crab Ocypode quadrata (Fabricius, 1787) in Southern Brazil

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