ABSTRACT. To determine how blood values in bottlenose dolphins changed during the year, 504 blood samples were taken from 9 dolphins from 1991 to 1999 and clinical blood examinations were undertaken monthly including 3 hematological and 19 serum chemistry tests. In creatinine, significant seasonal changes were found among three groups of adult males, adult females and juveniles, and the average values in summer were 15-38% higher than those in winter. In two out of three groups the average total cholesterol value were h ighest in winter, and the lowest of all groups were in summer. In two other groups the peaks of average FFA value were recorded in sum mer, and the lows were in winter. KEY WORDS: bottlenose dolphin, seasonal change, serum chemistry.J. Vet. Med. Sci. 64(11): 1075-1078, 2002 The physiology of free-living marine mammals adapts to seasonal environmental changes. It is known that bottlenose dolphins migrate seasonally, and the migration is probably a response to temperature changes [16,21]. Several physiological parameters, for example, the thickness of blubber [25], the body mass [21] and the rectal body temperature [23], are known to have seasonality even in captive dolphins.Seasonal changes in hematology have been recorded in birds [1,5,9,17,20,27] The blood samples taken from wild dolphins may be affected by several factors, including their handling stress and their diet. We previously reported how diet affected the blood composition of this species [24]. Therefore, it is necessary to examine the case of bottlenose dolphins in captivity. The information presented should help to establish a hematological base line of seasonal changes for bottlenose dolphins. It is important to be aware of seasonal changes for evaluating long-term physiological variations, for example, during pregnancy. Therefore, this report examines how blood values change seasonally in captive bottlenose dolphins, Tursiops truncatus, from the results of clinical blood examinations at Enoshima Aquarium from 1991 to 1999.Hematological data were compiled from the routine health records of 9 bottlenose dolphins (Table 1) which were divided into three groups: adult males (two dolphins, aged over 12 years), adult females (two dolphins, aged over 12 years), and juveniles (one male and four females, aged under 6 years) at Enoshima Aquarium, Kanagawa, Japan. This age classification was not changed throughout the study period. Three of these dolphins were born at this facility. The other six dolphins originated in waters around Japan. When they were brought into captivity, we estimated their ages from body length and body mass [22]. Clinical data over a 9-year period (1991 to 1999) for adult males and females, and a 5-year period (1995 to 1999) for juveniles were used, and a total of 504 blood samples were taken. Only blood samples from dolphins judged to be normal by behavior, appearance, or food consumption were used.The tank is outdoors with a total seawater volume of 5000 m 3 (45 m × 25 m, oval-shaped, with a depth of 3.5 to 5.5 m).