Seasonal variations in glycolysis in Mytilus galloprovincialis Lmk

Ibarguren, Izaskun; Vázquez-Illanes, Maria Dolores; Ramos-Martı́nez, Juan Ignacio

doi:10.1016/0305-0491(90)90281-w

Cited by 9 publications

(4 citation statements)

References 6 publications

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“…In addition, molluscan pyruvate kinase is regulated by the phosphorylation/dephosphorylation process (Marie et al, 1979;Siebenaller 1979;Kiener and Westhead, 1980;Nakashima et al, 1982;Hakim et al, 1984;Holwerda et al, 1989;Carillo et al, 2001). Moreover, there are many physiological factors such as temperature, anaerobic conditions, tidal cycle, seasonal and ion concentration variations that can alter pyruvate kinase activity in molluscs (Cortesi and Carpene, 1981;Carpene et al, 1984;Hakim et al, 1984;Cortesi et al, 1985;Ibarguren et al, 1990;Simpfendorfer et al, 1997).…”

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confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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SUMMARYThe present study investigates the transduction pathway mediated by cadmium in isolated digestive gland cells of mussel Mytilus galloprovincialis. The effects of cadmium treatment on a key glycolytic enzyme, pyruvate kinase (PK), and on Na+/H+ exchanger activity were examined. Cadmium (50 μmol l–1) caused a significant elevation of intracellular pH (pHi) and a rise (176%) of Na influx relative to control values. The amiloride analogue, EIPA (20 nmol l–1), a Na+/H+ exchanger blocker,together with cadmium, significantly reduced the effect of treatment by cadmium alone on both Na+ influx and pHi. In addition, PK activity was significantly increased after treatment with cadmium. PK activity was inhibited after treatment of cells with amiloride or EIPA together with cadmium. Moreover, phorbol-ester (PMA), a potent activator of protein kinase C(PKC), caused a significant rise in both pHi and PK activity, while staurosporine or calphostin C reversed both events. Adrenaline, isoprenaline and phenylephrine alone or together with cadmium also significantly increased the pHi and PK activity of isolated digestive gland cells. The latter effectors in combination with cadmium showed a synergistic effect on pHi and PK. These responses seem to be blocked by propranolol, metoprolol and prazosin. Our findings suggest a hormone-like effect of cadmium on digestive gland cells. The signal transduction pathway induced by cadmium involves the stimulation of PK, PKC and Na+/H+ exchanger in isolated digestive gland cells of Mytilus galloprovincialis.

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confidence: 99%

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Dailianis

Kaloyianni

2004

Journal of Experimental Biology

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“…Nevertheless, the said combination does not seem to be very suitable for purifying cGMP as well, because this nucleotide is removed by ZnSO 4 -Ba(OH) 2 (31). That is why we decided to use a combination of ZnSO 4 and Na 2 CO 3 , which forms ZnCO 3 and that had been proposed as a very adequate method to separate cAMP from AMP, ADP, and ATP (32). When applying that combination to a mixture that contained both cyclic nucleotides and the adenine 5Ј-ribonucleotides, under the conditions described in the legend to Fig.…”

Section: Extraction and Prepurification Of Camp And Cgmpmentioning

confidence: 96%

“…In this sense, we observed that the glycolytic pathway is particularly modulated in the mantle of Mytilus galloprovincialis Lmk. An increase in the glycolytic activity during the fall-winter season can be noted, coincidentally with the gonad development (3). On the other hand, the sea mussel is the model of facultative anaerobial invertebrate able to withstand very low levels of environmental oxygen, and even the lack of it, for long periods.…”

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confidence: 99%

Measurement of Adenosine 3′,5′-Cyclic Monophosphate and Guanosine 3′,5′-Cyclic Monophosphate in Mussel (Mytilus galloprovincialis Lmk.) by High-Performance Liquid Chromatography with Diode Array Detection

Enrich

Villamarı́n

Martinez

et al. 2000

Analytical Biochemistry

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“…Most of the work with mussels has been carried out on the common mussel M. edulis (2,5,7), whereas the metabolism of M. galloprovincialis has been little investigated (8)(9)(10). The specific features of the Black Sea population of M. galloprovincialis are linked to the absence of a tide cycle; usually mussels are not exposed to air under natural conditions, but these mussels can survive out of water for a few days.…”

Section: Introductionmentioning

confidence: 99%

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery

Lushchak

Bahnjukova²,

Spichenkov³

1997

Braz J Med Biol Res

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The modification of pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity in foot muscle of the mussel Mytilus galloprovincialis during exposure to air and recovery in water was investigated. In the course of exposure to air, the activity of these enzymes measured at high and low substrate concentrations showed successive increases and decreases. Returning the mussels to water after exposure to air affected enzyme activity in a manner similar to anaerobiosis. When measuring at saturated concentrations of substrates and substrate and coenzyme for PK and LDH, respectively, the maximum activation of PK (37%) was observed at 4 h of animal exposure to air, and for LDH (67%) at 6 h exposure to air. During 24 h of exposure of animals to air, PK activity practically reached the stock level, while LDH was still activated (148%). The change in lactate dehydrogenase activity in mussel muscle during anoxia and recovery is described here for the first time. Variation in pyruvate kinase activity during exposure to air and recovery is linked to the alteration of half-maximal saturation constants and maximal velocity for both substrates. The possible role of reversible phosphorylation in the regulation of pyruvate kinase and lactate dehydrogenase properties is discussed.

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Seasonal variations in glycolysis in Mytilus galloprovincialis Lmk

Cited by 9 publications

References 6 publications

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Cadmium induces both pyruvate kinase and Na+/H+exchanger activity through protein kinase C mediated signal transduction, in isolated digestive gland cells ofMytilus galloprovincialis(L.)

Measurement of Adenosine 3′,5′-Cyclic Monophosphate and Guanosine 3′,5′-Cyclic Monophosphate in Mussel (Mytilus galloprovincialis Lmk.) by High-Performance Liquid Chromatography with Diode Array Detection

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery

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