Seasonal Variation in the Antioxidant System of Eastern White Pine Needles

Anderson, James V.; Chevone, Boris I.; Hess, John L.

doi:10.1104/pp.98.2.501

Cited by 253 publications

(113 citation statements)

References 15 publications

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“…The supernatant was directly used for the determination of reduced ascorbate by HPLC and UV detection at 268 nm (Polle et al, 1992). For the quantification of total ascorbate, additional aliquots of the supernatant were oxidized, derivatized with o-phenylenediamine, and determined by fluorescence after HPLC separation (Polle et al, 1990) or alternatively reduced with 60 mM DTT in 200 mM 2-(N-cyclohexylamino)ethanesulfonic acid buffer, pH 9.3, for 1 h at room temperature and determined after HPLC separation at 268 nm (Anderson et al, 1992). Total glutathione, y-glutamylcysteine, and Cys were determined after reduction, derivatization with monobromobimanes, HPLC separation, and fluorescence detection (Schupp and Rennenberg, 1988).…”

Section: Extraction and Determination Of Antioxidantsmentioning

confidence: 99%

Responses of Antioxidative Systems to Drought Stress in Pendunculate Oak and Maritime Pine as Modulated by Elevated CO2

et al. 1996

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The aim of the present study was t o investigate the effects of an enhanced CO, concentration alone or in combination with drought stress on antioxidative systems of a deciduous (oak; Quercus robur) and an evergreen (pine; Pinos pinaster) tree species. The seedlings were grown for one season in a greenhouse in tunnels supplied with 350 or 700 p L L-' CO,. The experiment was repeated i n a second year. Antioxidants, protective enzymes, soluble protein, and pigments showed considerable fluctuations in different years. Elevated CO, caused significant reductions in the activities of superoxide dismutases i n both oak and pine. The activities of ascorbate peroxidase and catalase were also reduced in most cases. The activities of dehydroascorbate reductase, monodehydroascorbate radical reductase, glutathione reductase, and guaiacol peroxidase were affected little or not at all by elevated CO,. When the trees were subjected to drought stress by withholding water, the activities of antioxidative enzymes decreased in leaves of pine and oak grown at ambient CO, and increased in plants grown at elevated CO, concentrations. The present results suggest that growth i n elevated CO, might reduce oxidative stress to which leaf tissues are normally exposed and enhance metabolic flexibility t o encounter increased stress by increases in antioxidative capacity.Most predictions suggest that the current mean ambient CO, concentration of 355 pL L-' will approximately double by the end of the next century (Roeckner, 1992). Increasing concentrations of CO, and of other greenhouse gases will result in an increase in mean temperature and cause changes in precipitation patterns (Roeckner, 1992). Climate models predict large-scale drought periods during summer for northern mid-latitudes (Roeckner, 1992). The availability of water is one of the most important factors determining vegetation diversity and plant productivity (Rochefort and Woodward, 1992). The effects of water deficits on plant performance and growth are mediated through decreases in stomatal conductance and photosynthesis and depend on the severity and duration of the drought period, the '

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Section: Extraction and Determination Of Antioxidantsmentioning

confidence: 99%

Responses of Antioxidative Systems to Drought Stress in Pendunculate Oak and Maritime Pine as Modulated by Elevated CO2

et al. 1996

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“…Many long-lived evergreens respond to the onset of wintertime cold temperatures by decreasing chlorophyll content (Adams and Demmig-Adams 1994;Verhoeven et al 1996;Logan et al 1998c;Burkle and Logan 2003) and reorganizing their light harvesting antennae into an energy dissipating state (Gilmore and Ball 2000; Matsubara et al 2002;Adams et al 2002Adams et al , 2004. However, profound up-regulation of levels of antioxidants is another hallmark of acclimation to cold temperatures (Scho¨ner and Krause 1990;Anderson et al 1992;Mishra et al 1993;Logan et al 1998cLogan et al , 2003. Cold-induced increases in the levels of antioxidant enzymes compensate for the effect of lower temperatures on their activities and also presumably help cope with an enhanced rate of ROS formation (Lohrmann et al 2004).…”

Section: The Water-water Cyclementioning

confidence: 99%

The role of antioxidant enzymes in photoprotection

et al. 2006

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The enzymatic component of the antioxidant system is discussed as one of the defensive mechanisms providing protection against excessive light absorption in plants. We present an analysis of attempts to improve stress tolerance by means of the creation of transgenic plants with elevated antioxidant enzyme activities and conclude that the effect of such transgenic manipulation strongly depends on the manner in which the stress is imposed. The following factors may diminish the differences in photosynthetic performance between transgenic plants and wild type under field conditions: effective functioning of the thermal dissipation mechanisms providing a primary line of defense against excessive light, long-term adjustments of the antioxidant system and other photoprotective mechanisms, the relatively low level of control over electron transport exerted by the Water-Water cycle, especially under warm conditions, and a decrease in the content of the transgenic product during leaf aging.

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“…The determination of Cys was based on the reactivity of its sulfhydryl group with methyglyoxal as described by De Kok et al (1988). Total GSH (measured as total thiol) was determined according to Brehe and Burch (1976) as modified by Anderson et al (1992).…”

Section: Plant Analysismentioning

confidence: 99%

Boron Increases Synthesis of Glutathione in Sunflower Plants Subjected to Aluminum Stress

2006

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Ionic stress caused by high aluminum (Al) concentrations is one of the most widespread phytotoxicity problems globally in agricultural regions, greatly limiting crop yield in affected areas. The objective of this work was to examine a possible involvement of boron (B) in the detoxification of Al by stimulating glutathione (GSH) metabolism, a mechanism essential for the resistance of plants under stress conditions. Our results clearly demonstrate that increased application of B in the presence of high Al concentrations in the growth medium stimulates GSH biosynthesis, suggesting it could be an effective strategy to combat stress associated with the formation of active-oxygen species (AOS). In the specific case of Al toxicity, B reduces phytotoxicity by stimulating leaf biosynthesis of GSH and an increase in its concentration in the roots. Therefore, in this work, we also identify GSH metabolism as one of the key processes in Al detoxification. Finally, our results imply that greater B application leads to a greater resistance to Al toxicity, a fact that might be significant for higher productivity of agricultural plants grown in acid soils.

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Seasonal Variation in the Antioxidant System of Eastern White Pine Needles

Cited by 253 publications

References 15 publications

Responses of Antioxidative Systems to Drought Stress in Pendunculate Oak and Maritime Pine as Modulated by Elevated CO2

Responses of Antioxidative Systems to Drought Stress in Pendunculate Oak and Maritime Pine as Modulated by Elevated CO2

The role of antioxidant enzymes in photoprotection

Boron Increases Synthesis of Glutathione in Sunflower Plants Subjected to Aluminum Stress

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