Seasonal Variability in Cryoresistance of Saanen Goats Spermatozoa and Reproductive Characteristics

Bogdaniuk, Anastasiia; Garkavii, Volodymyr; Petrushko, Maryna

doi:10.15407/cryo32.01.034

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…To ensure a high fertilization rate after cryopreservation, spermatozoa must have a high cryotolerance. Our previous studies have shown that to achieve higher fertilization rate using cryopreserved sperm of Saanen goats, it is recommended to collect semen during the breeding season [2]. In general, sperm resistance to cryodamage is associated with seminal plasma proteins that provide protection against oxidative stress during cryopreservation [18].…”

Section: Resultsmentioning

confidence: 99%

“…А. О. Богданюк 1,2 , В. В. Гаркавий 3 , М. П. Петрушко 1 baa1995ua@gmail.com 1 Інститут проблем кріобіології і кріомедицини НАН України, вул. Переяславська, 23, м. Харків, 61016, Україна 2 ТОВ «Інститут Сучасних Ветеринарних Технологій», вул.…”

Section: репродуктивні характеристики цапів зааненської та альпійсько...unclassified

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Reproductive characteristics of Saanen and Alpine bucks

Bogdaniuk,

Garkavii,

Petrushko

2023

Bìol. Tvarin

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Genetic variations among breeds within a species can impact not only productivity traits, such as milk yield and quality, but also animal health, including fertility. This study aimed to compare the reproductive characteristics of bucks from the Saanen and Alpine breeds. Sperm concentration and motility were assessed using light microscopy, viability was determined using eosin-nigrosin staining, and morphological parameters were evaluated using the Spermac Stain method. DNA fragmentation was measured using the Halosperm kit. Artificial insemination of goats was conducted with fresh semen during natural estrus. Statistical analysis was performed using the Graph Pad Prism software. The results revealed that Alpine bucks exhibited significantly higher semen volume, sperm concentration, viability, and motility (P<0.05). No significant differences (P≥0,05) were observed between the breeds regarding the number of spermatozoa with normal morphology and the rate of DNA fragmentation. Cryobiological analysis of spermatozoa from Saanen bucks suggested a higher cryoresistance compared to the Alpine breed. Following artificial insemination of goats, the pregnancy rate for the Saanen breed was 61.8%, which was twice as high as that observed in Alpine goats — 28.8% (P<0.05). These findings demonstrate significant differences in reproductive characteristics between Saanen and Alpine goats. Despite superior sperm characteristics, the pregnancy rate after artificial insemination was significantly lower in the Alpine breed compared to the Saanen breed. Consequently, it is crucial to consider these variations in essential reproductive characteristics when implementing breeding programs and employing reproductive biotechnology in animal husbandry to ensure their successful application and effectiveness.

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Section: Resultsmentioning

confidence: 99%

Section: репродуктивні характеристики цапів зааненської та альпійсько...unclassified

Reproductive characteristics of Saanen and Alpine bucks

Bogdaniuk,

Garkavii,

Petrushko

2023

Bìol. Tvarin

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Morphological and morphometric characteristics of fresh and cryopreserved spermatozoa of saanen bucks

Bogdaniuk

Petrushko

2023

Agric. sci. pract.

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Background. Cryopreservation of spermatozoa with the best morphofunctional characteristics may allow their use for assisted reproductive technologies in goat breeding throughout the year to effectively increase livestock. Aim. To evaluate the influence of seasons on morphological and morphometric characteristics of fresh and cryopreserved goat sperm. Methods. The ejaculate samples of three sexually mature bucks of the Saanen breed were obtained during the breeding and non-breeding seasons. To assess the morphological characteristics, the smears of sperm were stained and then visualized under a light microscope with a magnification of ×1,000. For morphometric measurements, sperm micrographs were taken and analyzed using the ImageJ software. The cryopreservation of sperm was performed in a medium with 10 % glycerol and 20 % egg yolk. The samples were thawed in a water bath, then the morphological and morphometric characteristics of the cells were evaluated. Results. After the cryopreservation, the number of sperm with abnormal morphological structures was significantly higher compared to fresh sperm, both in the breeding and non-breeding seasons (p ≤ 0.05). After the cryopreservation, the length and width of the head decreased significantly (p ≤ 0.05) compared to the corresponding sperm sizes before the cryopreservation. Analyzing the morphological char- acteristics of the tail part, we revealed the fact of its reduction after the cryopreservation, probably due to twisting, loop formation, and detachment of its part. Conclusions. It was found that in the non-breeding season, the number of spermatozoa with damaged morphological structures increases, compared to the breeding season, which must be taken into account when using reproductive technologies. The morphological and morphometric characteristics of Saanen buck spermatozoa change after the cryopreservation, regardless of the season, which indicates the need to choose the fertilization tactics that will ensure the selection of spermatozoa without any morphological abnormalities.

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Sperm cryoresistance of different animal species

Maksimova,

Korochkina

2024

jour

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The aim of this work was the systematization and analysis of scientific data on cryoresistence of sperm of various types of animals and factors affecting the cryorezistivity of sperm. Sperm cryoresistence is the stability of sperm to the effects of low temperatures. Cryoresistence is evaluated according to the morphological characteristics of sperm, their activity and mobility after thawing, the integrity of the acrosomes, the plasma membrane and the degree of damage to chromatin. Cryoconservational is an important component of artificial insemination of animals, and knowledge about the cryorezistence of spermatozoa is the theoretical basis for the successful cryoponement of sperm. Based on the listed studies, cryoresistence has species features and depends on: the age of animals, pedigree features, season of the year, concentration of testosterone and prolactin in the blood, temperature and humidity index, as well as from cryoconservative protocols.

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Seasonal Variability in Cryoresistance of Saanen Goats Spermatozoa and Reproductive Characteristics

Cited by 4 publications

References 32 publications

Reproductive characteristics of Saanen and Alpine bucks

Reproductive characteristics of Saanen and Alpine bucks

Morphological and morphometric characteristics of fresh and cryopreserved spermatozoa of saanen bucks

Sperm cryoresistance of different animal species

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