Seasonal shift in gut microbiome diversity in wild Sichuan takin (Budorcas tibetanus) and environmental adaptation

Guan, Tian-Pei; Teng, Jade L. L.; Fong, Jordan YH; Lau, Susanna K. P.; Woo, Patrick C. Y.

doi:10.1016/j.csbj.2022.12.035

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…Clostridia_vadinB60_ group_unclassified belongs to the Clostridia_vadinB60_group, which is composed of conditional pathogens. The abundance of Acinetobacter increases significantly in winter, but its pathogenicity has not been discussed, and the abundance of this phylum in the current study was similar to that reported previously (Guan et al, 2022). Current research has found that the abundance of Clostridia_vadinB60_group positively correlated with the fasting insulin level and HOMA-IR, especially in overweight pregnant women or patients with preeclampsia (Yao et al, 2020).…”

Section: Figuresupporting

confidence: 86%

Analysis of the microbial diversity in takin (Budorcas taxicolor) feces

Ma,

Wang,

Cai

et al. 2023

Front. Microbiol.

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IntroductionThe intestinal tract of animals is a complex and dynamic microecosystem that is inextricably linked to the health of the host organism. Takin (Budorcas taxicolor) is a threatened species, and its gut microbiome is poorly understood. Therefore, this study aimed to analyze the microbial community structure and potential pathogens of takin.MethodsTakin fecal samples were collected from five sites in a nature reserve to ensure the uniformity of sample collection, determine the effects of different geographical locations on gut microbes, and analyze the differences in microbial communities between sites. Subsequently, high-throughput 16S rDNA gene sequencing was performed to analyze the microbial diversity and potential pathogens in the gut; the findings were verified by isolating and culturing bacteria and metagenomic sequencing.Results and discussionThe takin gut microflora consisted mainly of four phyla: Firmicutes (69.72%), Bacteroidota (13.55%), Proteobacteria (9.02%), and Verrucomicrobiota (3.77%), representing 96.07% of all microorganisms. The main genera were UCG-005 (20.25%), UCG-010_unclassified (12.35%), Firmicus_unclassified (4.03%), and Rumino coccsea_unclassified (3.49%), while the main species were assigned to Bacteria_unclassified. Potential pathogens were also detected, which could be used as a reference for the protection of takin. Pseudomonas presented the highest abundance at Shuichiping and may represent the main pathogen responsible for the death of takin at the site. This study provides an important reference for investigating the composition of the bacterial community in the intestine of takin.

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Section: Figuresupporting

confidence: 86%

Analysis of the microbial diversity in takin (Budorcas taxicolor) feces

Ma,

Wang,

Cai

et al. 2023

Front. Microbiol.

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“…The bulk of current literature addresses only ruminant fermentation ( Mura et al, 2019 ). Although the bacterial community composition of B. taxicolor has received considerable research attention, the characterization of fungal colonization has been relatively neglected ( Guan et al, 2023 ; Ma et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of fungal diversity in the gut feces of wild takin (Budorcas taxicolor)

Ma,

Li,

Cai

et al. 2024

Front. Microbiol.

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IntroductionThe composition of the intestinal microbiome correlates significantly with an animal’s health status. Hence, this indicator is highly important and sensitive for protecting endangered animals. However, data regarding the fungal diversity of the wild Budorcas taxicolor (takin) gut remain scarce. Therefore, this study analyzes the fungal diversity, community structure, and pathogen composition in the feces of wild B. taxicolor.MethodsTo ensure comprehensive data analyses, we collected 82 fecal samples from five geographical sites. Amplicon sequencing of the internal transcribed spacer (ITS) rRNA was used to assess fecal core microbiota and potential pathogens to determine whether the microflora composition is related to geographical location or diet. We further validated the ITS rRNA sequencing results via amplicon metagenomic sequencing and culturing of fecal fungi.Results and discussionThe fungal diversity in the feces of wild Budorcas taxicolor primarily comprised three phyla (99.69%): Ascomycota (82.19%), Fungi_unclassified (10.37%), and Basidiomycota (7.13%). At the genus level, the predominant fungi included Thelebolus (30.93%), Functional_unclassified (15.35%), and Ascomycota_unclassified (10.37%). Within these genera, certain strains exhibit pathogenic properties, such as Thelebolus, Cryptococcus, Trichosporon, Candida, Zopfiella, and Podospora. Collectively, this study offers valuable information for evaluating the health status of B. taxicolor and formulating protective strategies.

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