Abstract. A reconstruction of hydroclimate with an annual or sub-annual resolution covering the entire Holocene for a geographically limited region would significantly improve our knowledge of past climate dynamics, but has not been developed so far. With the use of an extensive collection of oak total ring-width series (Quercus robur and Quercus petraea) from living trees, historic timbers and subfossil alluvial wood deposits from the Main River region in southern Germany, a regional, 2,000-year long, seasonally-resolved hydroclimate reconstruction for the Main region has been developed. Climate-growth response analysis has been performed with daily climate records from AD 1900 onwards. An innovative analysis method for testing the stability of the developed transfer function (bootstrapped transfer function stability test, BTFS) as well as a classical calibration/verification approach have been implemented to study climate-growth model performance. Living oak trees from the Main River region show a significant sensitivity to precipitation sum from February 26 to July 06 (spring to mid-summer) during the full (r = 0.49, p