Seasonal Patterns of Respiratory Syncytial Virus, Influenza A Virus, Human Metapneumovirus, and Parainfluenza Virus Type 3 Infections on the Basis of Virus Isolation Data between 2004 and 2011 in Yamagata, Japan

Mizuta, Katsumi; Abiko, Chieko; Aoki, Yoko; Ikeda, Tatsuya; Matsuzaki, Yoko; Itagaki, Tsutomu; Katsushima, Fumio; Katsushima, Yuriko; Noda, Masahiro; Kimura, Hirokazu; Ahiko, Tadayuki

doi:10.7883/yoken.66.140

Cited by 51 publications

(48 citation statements)

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“…It has been shown that respiratory syncitial virus and influenza make a peak in winter months and RSV generally eme rges earlier (16). In a study conducted in Japan between 2004 and 2011 in which 13 325 nasal samples most of which were obtained from subjects aged below 5 years were examined, it was reported that RSV infections commonly occured at the end of the year, influenza A infections commonly occured between January and March, IMPV infections commonly occured between March and April and parainfluenza type 3 infections commonly occured between May and July (29). Although our study could not give a full seasonal distribution, because it did not cover a whole year, influenza, RSV, IMPV infections were observed most commonly in January during our study period.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of epidemiological and clinical features of influenza and other respiratory viruses

Aktürk¹,

Sütçü²,

Badur³

et al. 2015

Turk Pediatri Ars

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Aim: In our study, we aimed to clinically and epidemiologically evaluate respiratory tract infections the viral agents of which were detected by molecular methods and to compare influenza and other respiratory tract viruses in this context. Material and Methods:The records of 178 patients aged above 2 years who presented to pediatric emergency outpatient clinic with fever and respiratory tract infection findings between December 2013 and April 2014 were examined retrospectively. Results: At least one respiratory tract pathogen was detected by polymerase chain reaction in 78.6% (n=140) of the patients: influenza A 33.5%, influenza B 16.4%, respiratory syncytial virus 9.2%, adenovirus 7.8%, rhinovirus 7.1%, coronavirus 7.1%, human metapneumovirus 5.7%, human bocavirus 5.7%, parainfluenza virus 3.5%, coinfection 2.8%. The mean age of the patients was 6.3±3.6 years. Sixty-nine patients (49.2%) were aged between 2 and 5 years. Seventy-one patients (50.7%) were aged 5 years and above. Upper respiratory tract infection was found with a rate of 65.7% and lower respiratory tract infection was found with a rate of 34.2%. It was observed that the distribution of respiratory tract viruses showed variance by age groups. Influenza A infection was observed with the highest rate in both age groups. Influenza B was the second leading agent (p=0.008) above the age of 5 years and respiratory syncytial virus was the second leading agent in the 2-5 year age group (p=0.003). Influenza viruses were detected in 55.9% of 118 patients who were found to be compatible with the definition of "influenza-like illness" specified in the Center for Disease Control and Prevention guidelines and other viral agenst were detected in 44%. No difference could be found between the clinical pictures and radiological findings caused by influenza and other respiratory tract viruses. Conclusions: In this study, it was concluded that influenza and other respiratory viruses can not be differentiated definitely by clinical and radiological findings, though there are some differences. (Turk Pediatri Ars 2015; 50: 217-25)

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Section: Discussionmentioning

confidence: 99%

Evaluation of epidemiological and clinical features of influenza and other respiratory viruses

Aktürk¹,

Sütçü²,

Badur³

et al. 2015

Turk Pediatri Ars

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“…In addition, metapneumovirus (5.1%) was more commonly seen in the winter. In the literature Waston and Eglin showed that RSV infections occurred more frequently in the winter, while Parainfluenza Type 3 infections occurred more often in the spring and summer between 1978 and 1987 in the United Kingdom (39,42). In their study conducted in the United States, Iwane et al (43) reported that RSV infections peaked in January 2001, Influenza peaked in February 2001, and parainfluenza peaked in March-May and August-September in 2002.…”

Section: Discussionmentioning

confidence: 98%

“…These symptoms are much more common in upper respiratory system infections than in lower respiratory system infections. Many respiratory viruses cause similar symptoms, and infected patients are often diagnosed with colds, pharyngitis, bronchitis, croup, bronchiolitis, and pneumonia (39). Thus, in cases of acute respiratory system infection, it is difficult to make a differential diagnosis with regards to the causative agent in a clinical setting (39).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, in cases of acute respiratory system infection, it is difficult to make a differential diagnosis with regards to the causative agent in a clinical setting (39). Making a clinical diagnosis while keeping in mind epidemiological considerations allows the clinician to make a better decision regarding the selection of the appropriate specimens and tests (39).…”

Section: Discussionmentioning

confidence: 99%

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Respiratory Viral Test Results in Children

Dut¹,

Kocagöz²

2017

Çocuk Enf Derg

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ÖzetAmaç: Akut solunum yolları enfeksiyonları benzer semptomlarla karakterize respiratuvar viruslerle ilişkili-dir. Bundan dolayı, viral enfeksiyonun tanısı respiratuvar virusun hem klinik hem de epidemiyolojik özellikle-ri ile konulur. Bu çalışmada, çocuklarda benzer şika-yetlerle alınan viral test sonuçlarının incelenmesi planlanmıştır. Yöntemler: Şubat 2012 -Şubat 2014 tarihleri arasında Acıbadem Maslak Hastanesinde akut solunum yolu enfeksiyonu tanısı ya da ilişkili durumlarda respiratuvar viral test (RVT) alınmış 312 çocuğun test sonucunu retrospektif olarak inceledik. Bulgular: RVT'lerin 155 (%49.6) pozitif; 135' inde (%43,2) sadece tek virus tespit edilirken 20 (%6,4) inde iki virus tespit edilmiştir. RVT pozitif olanlarda, 29 (%9,2) RSV, 18 (%5.79) adenovirus, 11 (%3,53) inde coronavirus, 10 (%3,2) inde parainfluenza ve 7 (%2,2) inde metapnömovirus tespit edilmiştir. Sonuç: Solunum yolları viral enfeksiyon epidemiyolojisi, toplumlara, coğrafik konuma, yıllara, yılın belirli zamanlarına, mevsimlere ve yaş gibi birçok değişken-den etkilenmektedir. Lokal bir hastanede, ülkemizde çocuk yaş grubunda, akut solunum yolu enfeksiyonu ya da benzer durumlarda alınan RVT sonuçlarını inceledik. Bu epidemiyolojik sonuçların bilinmesi, yıllık enfeksiyon önleme; gerekli tedbirlerin alınması; uygulamalar için önemlidir. (J Pediatr Inf 2016; 10: 119-27) Anahtar kelimeler: Viral, test, çocuk AbstractObjective: Acute respiratory system infections (ARI) are thought to be associated with respiratory viruses characterized by similar symptoms. Therefore, the diagnosis of a viral infection must include a thorough assessment of both the clinical and epidemiologic features of respiratory viruses. The aim of this study to evaluate of similar complaints of children viral test results. Methods: We retrospectively investigated a total of 312 respiratory viral test (RVT) results of children with acute respiratory system infection or related conditions between February 2012 and February 2014. Results: Of the 312 RVT results, 155 were positive (49.6%); 135 (43.2%) of these detected only one virus and 20 (6.4%) detected two viruses. One hundred and fifty (48.0%) of the cases resulted in hospital admission, while 162 (51.2%) did not. Thirty (9.6%) of the positive results were treated in the observation unit. Of the positive RVT tests, 29 (9.2%) detected RSV, 18 (5.79%) detected adenovirus (all types), 11 (3.53%) detected coronavirus, 10 (3.2%) detected parainfluenza (all types), and 7 (2.2%) detected metapneumovirus. Conclusion: Epidemiological features related to age, season, time of year, year to year, geographic location, and population affect respiratory viral infections. Therefore, we suggest that epidemiological surveys of ARIs, both longitudinally and locally, should be performed. (J Pediatr Inf 2016; 10: 119-27

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“…Like other respiratory viruses, human metapneumovirus (hMPV) causes severe disease in children, the elderly, and people with chronic diseases such as asthma, emphysema, or who are immune compromised. Several studies report that hMPV has an infection peak between 6 and 12 months of age, unlike the RSV infection peak, occurring at 2–3 months of age [2, 3]. The virus is rarely detected in healthy children, but 40% of severe acute respiratory cases and hospitalized children has hMPV [4].…”

Section: Introductionmentioning

confidence: 99%

Human Metapneumovirus: Laboratory Methods for Isolation, Propagation, and Plaque Titration

et al. 2018

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The human metapneumovirus (hMPV) is an important viral agent associated with severe infections of the upper and lower airways, especially in young children and immunosuppressed subjects. Nevertheless, in vitro studies of hMPV are very difficult due to the little knowledge we have on its laboratory manipulation. Objective: The aim of this study was to isolate and propagate hMPV from patients, and to establish a method to quantify the virus by plaque assay. Method: As part of a Latin American respiratory virus surveillance study, 12 nasal secretion samples – hMPV-positive by direct fluorescence – were inoculated on LLC-MK2 cells to isolate the virus. The supernatants were re-inoculated and the cytopathic effect and syncytium formation were evaluated daily; the infection was confirmed by immunofluorescence and RT-PCR. A protocol to titrate the harvested virus was established inoculating serial dilutions on LLC-MK2 cells, and agarose was then added as an overlay. After different time periods, the monolayers were fixed and stained with Naphthol blue/black or crystal violet and finally the viral titer was obtained. Results: Eight out of 12 hMPV-positive respiratory samples were positive for the isolation and confirmed by RT-PCR and immunofluorescence, but the cytopathic effect and syncytium formation were observed only in 5 cultures. One out of 8 viral isolates was used for propagation and plaque assay standardization. We found that incubation for 7 days in the semisolid overlay yielded plaques with appropriate size and shape to be counted, although crystal violet staining showed slightly larger plaques than those seen with Naphthol blue/black staining. Conclusions: The isolation and propagation from patient-derived hMPV and the standardization of a practical, reliable, and inexpensive method of detection and quantification of hMPV were carried out, without the additional use of antibodies that had not been reported previously. These results offer some important insights for future studies of cellular and molecular biology of hMPV.

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Seasonal Patterns of Respiratory Syncytial Virus, Influenza A Virus, Human Metapneumovirus, and Parainfluenza Virus Type 3 Infections on the Basis of Virus Isolation Data between 2004 and 2011 in Yamagata, Japan

Cited by 51 publications

References 20 publications

Evaluation of epidemiological and clinical features of influenza and other respiratory viruses

Evaluation of epidemiological and clinical features of influenza and other respiratory viruses

Respiratory Viral Test Results in Children

Human Metapneumovirus: Laboratory Methods for Isolation, Propagation, and Plaque Titration

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