Seasonal patterns in microbial communities inhabiting the hot springs of <scp>T</scp>engchong, <scp>Y</scp>unnan Province, <scp>C</scp>hina

Briggs, Brandon R.; Brodie, Eoin L.; Tom, Lauren M.; Dong, Hailiang; Jiang, Hongchen; Huang, Qiuyuan; Wang, Shang; Hou, Weiguo; Wu, Geng; Huang, Liuquin; Hedlund, Brian P.; Zhang, Chuanlun; Dijkstra, Paul; Hungate, Bruce A.

doi:10.1111/1462-2920.12311

Cited by 57 publications

(79 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supporting our assumption that hot springs of this nature are low in biomass, a recently concluded PhyloChipbased study reported the presence of only 4882 OTUs associated with a Chinese hot spring (Briggs et al, 2013). As documented previously in Thailand's Bor Khlueng neutral pH hot spring systems (50-57°C), members of major phyla were well represented in the P1, P2, and P3 samples (Kanokratana et al, 2004).…”

Section: Discussionsupporting

confidence: 84%

Microbial Community Structures of Novel Icelandic Hot Spring Systems Revealed by PhyloChip G3 Analysis

et al. 2014

Self Cite

View full text Add to dashboard Cite

Microbial community profiles of recently formed hot spring systems ranging in temperatures from 57°C to 100°C and pH values from 2 to 4 in Hverager+i (Iceland) were analyzed with PhyloChip G3 technology. In total, 1173 bacterial operational taxonomic units (OTUs) spanning 576 subfamilies and 38 archaeal OTUs covering 32 subfamilies were observed. As expected, the hyperthermophilic (*100°C) spring system exhibited both low microbial biomass and diversity when compared to thermophilic (*60°C) springs. Ordination analysis revealed distinct bacterial and archaeal diversity in geographically distinct hot springs. Slight variations in temperature (from 57°C to 64°C) within the interconnected pools led to a marked fluctuation in microbial abundance and diversity. Correlation and PERMANOVA tests provided evidence that temperature was the key environmental factor responsible for microbial community dynamics, while pH, H 2 S, and SO 2 influenced the abundance of specific microbial groups. When archaeal community composition was analyzed, the majority of detected OTUs correlated negatively with temperature, and few correlated positively with pH.

show abstract

Section: Discussionsupporting

confidence: 84%

Microbial Community Structures of Novel Icelandic Hot Spring Systems Revealed by PhyloChip G3 Analysis

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…As a result, the formate and acetate assimilation and mineralization activities reported here may be more appropriately referred to as rate potentials rather than absolute rates. A further consideration which is supported by repeated sampling of hot springs over seasonal or annual cycles is that the concentrations of organic substrates vary temporally (25,47). This variability makes the estimation of representative substrate transformation rates more difficult and potentially less meaningful, as these rates likely vary to a large degree in accordance with variations in the mode and magnitude of delivery of organic substrates to these systems.…”

Section: Discussionmentioning

confidence: 99%

Carbon Source Preference in Chemosynthetic Hot Spring Communities

Urschel

Kubo

Hoehler

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Rates of dissolved inorganic carbon (DIC), formate, and acetate mineralization and/or assimilation were determined in 13 high-temperature (>73°C) hot springs in Yellowstone National Park (YNP), Wyoming, in order to evaluate the relative importance of these substrates in supporting microbial metabolism. While 9 of the hot spring communities exhibited rates of DIC assimilation that were greater than those of formate and acetate assimilation, 2 exhibited rates of formate and/or acetate assimilation that exceeded those of DIC assimilation. Overall rates of DIC, formate, and acetate mineralization and assimilation were positively correlated with spring pH but showed little correlation with temperature. Communities sampled from hot springs with similar geochemistries generally exhibited similar rates of substrate transformation, as well as similar community compositions, as revealed by 16S rRNA gene-tagged sequencing. Amendment of microcosms with small (micromolar) amounts of formate suppressed DIC assimilation in short-term (<45-min) incubations, despite the presence of native DIC concentrations that exceeded those of added formate by 2 to 3 orders of magnitude. The concentration of added formate required to suppress DIC assimilation was similar to the affinity constant (K m ) for formate transformation, as determined by community kinetic assays. These results suggest that dominant chemoautotrophs in hightemperature communities are facultatively autotrophic or mixotrophic, are adapted to fluctuating nutrient availabilities, and are capable of taking advantage of energy-rich organic substrates when they become available.

show abstract

“…Authors concluded that this is mainly due to differences in the pH, temperatures and TOC between the two springs (Hou et al 2013). The effect of seasonal changes on the microbial diversity in Tengchong hot springs, which are located in a subtropical area with heavy temporal monsoon rain fall, has been studied by Briggs et al (2014) and Wang et al (2014). Specifically, they compared the samples collected between June and August (rainy season) with those of Hou and colleagues sampled in January during the dry season.…”

Section: Tengchong Chinamentioning

confidence: 99%

Metagenomics of microbial and viral life in terrestrial geothermal environments

Strazzulli

Fusco

Cobucci‐Ponzano

et al. 2017

Rev Environ Sci Biotechnol

View full text Add to dashboard Cite

Geothermally heated regions of Earth, such as terrestrial volcanic areas (fumaroles, hot springs, and geysers) and deep-sea hydrothermal vents, represent a variety of different environments populated by extremophilic archaeal and bacterial microorganisms. Since most of these microbes thriving in such harsh biotopes, they are often recalcitrant to cultivation; therefore, ecological, physiological and phylogenetic studies of these microbial populations have been hampered for a long time. More recently, culture-independent methodologies coupled with the fast development of next generation sequencing technologies as well as with the continuous advances in computational biology, have allowed the production of large amounts of metagenomic data. Specifically, these approaches have assessed the phylogenetic composition and functional potential of microbial consortia thriving within these habitats, shedding light on how extreme physico-chemical conditions and biological interactions have shaped such microbial communities. Metagenomics allowed to better understand that the exposure to an extreme range of selective pressures in such severe environments, accounts for genomic flexibility and metabolic versatility of microbial and viral communities, and makes extreme-and hyper-thermophiles suitable for bioprospecting purposes, representing an interesting source for novel thermostable proteins that can be potentially used in several industrial processes.Keywords Hyperthermophiles Á Geothermal environments Á Microbial and viral metagenomics Á CRISPR Á Enzyme discovery BackgroundHot springs populated by extreme thermophiles (T opt : 65-79°C) and hyperthermophiles (T opt [ 80°C) (DeCastro et al. 2016) are very diverse and some of them show combinations of extreme chemo-physical conditions, such as temperature, acidic or alkaline pHs, high pressure, and high concentrations of salts and heavy metals (López-López et al. 2013). As with all studies of environmental microbiology, our understanding of the composition, functional and physiological dynamics, and evolution of extreme-and hyper-thermophilic microbial consortia has lagged A. Strazzulli Á M. Moracci Á P. Contursi

show abstract

Seasonal patterns in microbial communities inhabiting the hot springs of Tengchong, Yunnan Province, China

Cited by 57 publications

References 45 publications

Microbial Community Structures of Novel Icelandic Hot Spring Systems Revealed by PhyloChip G3 Analysis

Microbial Community Structures of Novel Icelandic Hot Spring Systems Revealed by PhyloChip G3 Analysis

Carbon Source Preference in Chemosynthetic Hot Spring Communities

Metagenomics of microbial and viral life in terrestrial geothermal environments

Contact Info

Product

Resources

About