Seasonal Nutrient Cycling and Enrichment of Nutrient-Related Soil Microbes Aid in the Adaptation of Ramie (Boehmeria nivea L.) to Nutrient-Deficient Conditions

Wu, Shenglan; Song, Xue; Iqbal, Yasir; Hu-cheng, Xing; Jie, Yucheng

doi:10.3389/fpls.2021.644904

Cited by 14 publications

(18 citation statements)

References 36 publications

(45 reference statements)

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“…The high‐throughput sequencing analysis generally included three steps: DNA extraction, PCR amplification and Illumina MiSeq sequencing. Detailed information on each step can be found in Wu et al (2021).…”

Section: Methodsmentioning

confidence: 99%

Quantitative assessment of the potential for soil improvement by planting Miscanthus on saline‐alkaline soil and the underlying microbial mechanism

Zheng

et al. 2021

GCB Bioenergy

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Phytoremediation using Miscanthus is a valuable technique for the restoration of saline‐alkaline soil, but the potential is not quantified. To assess this potential, a 5 years field experiment was conducted on saline‐alkaline soils with three genotypes of Miscanthus and an unplanted control. The bulk soil (0–20‐cm depth) physicochemical properties were measured annually from 2015 to 2018, while the bacterial and fungal community structures of the rhizosphere soil were investigated by high‐throughput sequencing after 5 years under Miscanthus (2018). The results showed that Miscanthus cultivation on saline‐alkaline soil reduced soil salinization and improved soil fertility on this site. Specifically, compared with the blank control, the five‐years Miscanthus cultivation (mean of three different genotypes pooled) decreased the soil pH by 0.33 units (8.78–8.45) and electrical conductivity by 23.4 μS/cm (226.7–203.3 μS/cm), as well as increasing soil organic matter content from 18.9 to 34.9 g/kg and total potassium content from 15.2 to 18.0 g/kg. In addition, the soil improvement was more pronounced with a longer duration of growth. Miscanthus remediates saline‐alkaline soils by altering the microbial community compositional abundance rather than its diversity. The improvement is due to enrichment of beneficial bacteria leading to soil desalinization (e.g., by Gemmatimonadaceae), litter decomposition (e.g., by Chitinophagaceae), plant growth promotion (e.g., by Micrococcaceae), and the reduction of harmful fungi (e.g., Pleosporales and Chitinophagaceae). Soil pH, electrical conductivity, organic matter, and total potassium were the main physicochemical properties affecting bacterial and fungal communities. Our results quantify the soil improvement potential of Miscanthus and clarify the underlying microbial mechanisms, laying a solid foundation for further implementation of saline‐alkaline soil phytoremediation using Miscanthus. In the future, more extensive multi‐location trials are required to assess if these improvements are seen across a wide diversity of environments.

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Section: Methodsmentioning

confidence: 99%

Quantitative assessment of the potential for soil improvement by planting Miscanthus on saline‐alkaline soil and the underlying microbial mechanism

Zheng

et al. 2021

GCB Bioenergy

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“…Soil microorganisms play a vital role in nutrient recycling [9]. During our previous research, the helpful roles of soil microbes in the adaptation of the ramie to poor soil conditions were confirmed [10]. This is generally achieved through enrichment of the beneficial bacteria and through a reduction of harmful fungi simultaneously.…”

Section: Introductionmentioning

confidence: 81%

“…Phosphate solubilizing microorganisms (PSM) can convert immobilized inorganic P to soluble organic P (i.e., P-solubilization), which increases the bioavailable P for plant uptake and utilization [13]. Our previous work already detected the N-fixing bacteria of Bradyrhizobium from the rhizosphere soil of ramie using sequencing technology [10]. However, the exact N-fixing potential is not clear.…”

Section: Introductionmentioning

confidence: 99%

“…To explore how the microorganisms affect the potential of N-fixing/P-solubilization (Objective III), the activities of the nutrient-related soil enzymes are also monitored and compared as a part of this study. Furthermore, based on the outcomes of the former research work [10], it is revealed that the harmful fungal communities (e.g., Cladosporium) can be enriched in the ramie rhizosphere soil, which can kill the beneficial bacteria and limit the N-fixing potential. Therefore, the fourth objective (Objective IV) is to verify the inhibitory effect of fungal communities on N-fixing bacterial communities in the rhizosphere.…”

Section: Introductionmentioning

confidence: 99%

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Role of Rhizosphere Soil Microbes in Adapting Ramie (Boehmeria nivea L.) Plants to Poor Soil Conditions through N-Fixing and P-Solubilization

Jie

2021

Agronomy

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The N-fixing and P-solubilization functions of soil microbes play a vital role in plant adaptation to nutrient-deficiency conditions. However, their exact roles toward the adaptation of ramie to poor soil conditions are still not clear. To fill this research gap, the N-fixing and P-solubilization efficiencies of soils derived from the rhizosphere of several ramie genotypes with different levels of poor soil tolerance were compared. Correlations between the N-fixing, P-solubilization efficiency, and the poor soil tolerable index were analyzed to quantify their contributions towards the adaptation of ramie plants to poor soil conditions. To explore how the microorganisms affected the potential of N-fixing/P-solubilization, the activities of the nutrients related the soil enzymes were also tested and compared. The results of this study confirm the existence of N-fixing and P-solubilization bacteria in the ramie rhizosphere of the soil. The number of N-fixing bacteria varied from 3010.00 to 46,150.00 c.f.u. per gram dry soil for the ramie treatment, while it was only 110.00 c.f.u. per gram dry soil for treatment without ramie cultivation. The average P-solubilization efficiency of ramie treatment was almost five times higher than that of the control soil (0.65 vs. 0.13 mg mL−1). The significant correlations between the poor soil tolerance index and the N-fixing bacteria number (r = 0.829)/nitrogenase activity (r = 0.899) suggest the significantly positive role of N-fixing function in the adaptation of ramie plants to poor soil. This is also true for P-solubilization, as indicated by the significant positively correlation coefficients between the ramie poor soil tolerance index and P-solubilization efficiency (0.919)/acid phosphatase activity (0.846). These characteristics would accelerate the application of “holobiont” breeding for improving ramie nutrient use efficiency.

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“…This result is in accordance with Bao et al (2019) , who reported that the dominant bacteria in a paddy soil included Bacillus , Acidobacteria/Gp1 , Acidobacteria/Gp3 , and Ktedonobacter . Wu et al (2021) found that the enrichment of beneficial bacteria, mainly Gaiella , contributed to the ability of ramie to tolerate poor soils. Our results showed that the cultivation of different cassava genotypes recruited different unique and core microbes to the cassava tubers and rhizospheres and that the microbes were significantly different in identity and relative abundance ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of the Bacterial Microbiome Associated With the Endosphere and Rhizosphere of Different Cassava (Manihot esculenta Crantz) Genotypes

Gao

Zhang

et al. 2021

Front. Microbiol.

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Root-associated microbial communities play important roles in plant growth and development. However, little attention has been paid to the microbial community structures associated with cassava, which is a staple food for approximately 800 million people worldwide. Here, we studied the diversity and structure of tuber endosphere and rhizosphere bacterial communities in fourteen cassava genotypes: SC5, SC8, SC9, SC205, KU50, R72, XL1, FX01, SC16, 4612, 587, 045, S0061, and 1110. The results of bacterial 16S rDNA sequencing showed that the richness and diversity of bacteria in the rhizosphere were higher than those in the tuber endosphere across the 14 cassava genotypes. After sequencing, 21 phyla and 310 genera were identified in the tuberous roots, and 36 phyla and 906 genera were identified in the rhizosphere soils. The dominant phylum across all tuber samples was Firmicutes, and the dominant phyla across all rhizosphere samples were Actinobacteria, Proteobacteria, and Acidobacteria. The numbers of core bacterial taxa within the tuber endospheres and the rhizospheres of all cassava genotypes were 11 and 236, respectively. Principal coordinate analysis and hierarchical cluster analysis demonstrated significant differences in the compositions of rhizosphere soil microbiota associated with the different cassava genotypes. Furthermore, we investigated the metabolic changes in tuber roots of three genotypes, KU50, SC205, and SC9. The result showed that the abundances of Firmicutes, Proteobacteria, and Actinobacteria in tuber samples were positively correlated with organic acids and lipids and negatively correlated with vitamins and cofactors. These results strongly indicate that there are clear differences in the structure and diversity of the bacterial communities associated with different cassava genotypes.

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Seasonal Nutrient Cycling and Enrichment of Nutrient-Related Soil Microbes Aid in the Adaptation of Ramie (Boehmeria nivea L.) to Nutrient-Deficient Conditions

Cited by 14 publications

References 36 publications

Quantitative assessment of the potential for soil improvement by planting Miscanthus on saline‐alkaline soil and the underlying microbial mechanism

Quantitative assessment of the potential for soil improvement by planting Miscanthus on saline‐alkaline soil and the underlying microbial mechanism

Role of Rhizosphere Soil Microbes in Adapting Ramie (Boehmeria nivea L.) Plants to Poor Soil Conditions through N-Fixing and P-Solubilization

Diversity of the Bacterial Microbiome Associated With the Endosphere and Rhizosphere of Different Cassava (Manihot esculenta Crantz) Genotypes

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