Seasonal microbial profiling of left kidney and stomach of farmed adult greenlip abalone (
            <i>Haliotis laevigata</i>
            )

Wang, Yanhui; Pyecroft, Stephen; Barton, Mary D.

doi:10.1111/are.15060

Cited by 4 publications

(4 citation statements)

References 51 publications

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Section: Discussionsupporting

confidence: 92%

“…High-throughput sequencing technology can better visualize the microbial communities associated with abalone farming. Results showed that Proteobacteria and Bacteroidetes are the dominant bacteria and most efficient colonizers in abalone farming waters, which is consistent with previous studies [27]. In contrast to terrestrial animals, microorganisms in the aquatic environment have a significant impact on the bacterial composition of aquatic animals due to their constant contact with the surrounding aquatic environment [28][29][30].…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Monitoring Bacterial Community Dynamics in Abalone (Haliotis discus hannai) and the Correlations Associated with Aquatic Diseases

Zhang

Zhu

Wang

et al. 2022

Water

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Bacteria are an integral component of their host. However, information about the microbiota living in and around many aquatic animals is lacking. In this study, multiplex bar-coded pyrosequencing of the 16S ribosomal RNA gene was used to monitor the dynamics of abalone, Haliotis discus hannai, bacterial communities in the intestine, water from cement culture ponds, and surrounding sea areas. Correlations between the bacterial communities and common aquaculture diseases were also evaluated. A total of 329,798 valid sequences and 15,277 operational taxonomic units (OTUs) from 32 samples were obtained by 454 tag amplicon pyrosequencing. The Shannon indices of the seawater samples ranged from 2.84 to 5.6 and the Shannon indices of the abalone intestine samples ranged from 1.2 to 5.12, which were much lower than those of seawater. The dominant phyla in seawater samples were Proteobacteria, Bacteroidetes, Fusobacteria, Cyanobacteria, etc. The dominant phyla in the abalone intestine varied greatly in different months. The dominant genera in the seawater of the cement culture ponds changed in different months, mainly Psychrilyobacter and Pseudoalteromonas. The dominant genera in seawater from the open sea vary considerably between months. The dominant genus of bacteria in the abalone intestine during the months when abalones are susceptible to disease is mainly Mycoplasma spp. Canonical correspondence analysis revealed that bacterial communities in seawater and the intestine responded differently to environmental variables, with similar microbiota in the same area. pH, dissolved oxygen concentration, and temperature were closely related to the samples from the sea area. Oxidation-reduction potential, salinity, phosphate, nitrate, and ammonia nitrogen concentrations were closely related to the water samples from the artificial pools. These findings may add significantly to our understanding of the complex interactions between microbiota and environmental variables in the abalone intestine as well as in the surrounding seawater.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Monitoring Bacterial Community Dynamics in Abalone (Haliotis discus hannai) and the Correlations Associated with Aquatic Diseases

Zhang

Zhu

Wang

et al. 2022

Water

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“…1B ). Meanwhile, previous studies on gastrointestinal bacterial communities from abalones with different species ( 3 , 9 – 11 , 24 ), geographical locations ( 3 , 9 , 10 , 24 , 25 ), and diets ( 3 , 11 , 24 ) also support that Psychrilyobacter is a core predominant member, even reaching over 90% in some species, such as H. tuberculate ( 3 ).…”

Section: Resultsmentioning

confidence: 84%

Genomic and metabolic insights into the first host-associated isolate of Psychrilyobacter

Liu,

Wei,

Lai

et al. 2023

Microbiol Spectr

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Although gut bacteria are vital to their hosts, few studies have focused on marine animals. Psychrilyobacter is frequently related to various marine animals, but its interaction with host remains unknown due to the lack of host-associated isolate or genomic information. Here, we combined cultivation-independent and cultivation-dependent methods to uncover the potential roles of Psychrilyobacter in the host abalone. The high-throughput sequencing and literature compiling results indicated that Psychrilyobacter is widely distributed in marine and terrestrial ecosystems with both host-associated and free-living lifestyles, but with a strong niche preference in the guts of marine invertebrates, especially abalone. By in vitro enrichment that mimicked the gut inner environment, the first host-related pure culture of Psychrilyobacter was isolated from the abalone intestine. Phylogenetic, physiological, and biochemical characterizations suggested that it represents a novel species named Psychrilyobacter haliotis B1. Carbohydrate utilization experiments and genomic evidence indicated that B1 can utilize diverse host-food-related monosaccharides and disaccharides but not polysaccharides, implying its potential role in the downstream fermentation instead of the upstream food degradation in the gut. Particularly, this strain showed potential to colonize the gut and benefit the host via different strategies, such as the short-chain fatty acids generation by fermenting peptides and/or amino acids, and the putative production of diverse vitamins and antibiotics to support the host growth and antipathogenicity. To our knowledge, strain B1 represents the first host-related pure culture of Psychrilyobacter ; genomic and metabolic evidence showed some beneficial characteristics of the dominant gut anaerobe to the host. IMPORTANCE Psychrilyobacter is a globally distributed bacterial genus and with an inhabiting preference for guts of marine invertebrates. Due to the difficulty of cultivation and the limited genomic information, its role in host remains largely unknown. We isolated the first host-associated Psychrilyobacter species from abalone gut and uncovered its functional potential to the host through different mechanisms. Our findings provide some insights into the understanding of host-microbe interactions on a core taxon with the marine invertebrates, and the isolate may have an application potential in the protection of marine animals.

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“…1B). Meanwhile, previous studies on gastrointestinal bacterial communities from abalones with different species (3,(9)(10)(11)24), geographical locations (3,9,10,24,25) and diets (3,11,24) also support that Psychrilyobacter is a core predominant member, even reaching over 90% in some species, such as Haliotis tuberculate (3).…”

Section: Psychrilyobacter Dominated the Abalone Gut And Was Widely Di...mentioning

confidence: 91%

The first host-associated anaerobic isolate ofPsychrilyobacterprovides insights into its potential roles in the abalone gut

Liu

Wei

Lai

et al. 2022

Preprint

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Gut bacteria are essential to their hosts, but few studies have focused on marine animals.Psychrilyobacteris frequently related to various marine animals, but the relationship with host remains unknown due to lack of host-associated isolate or genomic information. Here, we combined of high-throughput sequencing, isolating and genomic analyses to uncover its potential role in host abalone. The high-throughput sequencing and literature compiling results indicated thatPsychrilyobacteris widely distributed in marine and terrestrial ecosystems with both host-associated and free-living lifestyles. It showed a strong preference for the guts of marine invertebrates, especially abalone, which generally persisted with high relative abundances. By mimicking the gut inner environment for enrichment, the first host-related pure culture ofPsychrilyobacterwas isolated from the abalone intestine. Phylogenetic, physiological and biochemical characterizations suggested that it represents a novel species namedPsychrilyobacter haliotisB1. Carbohydrate utilization experiments and genomic evidence indicated that B1 is good at fermenting diverse host-food-related monosaccharides and disaccharides but not polysaccharides, implying its critical roles in downstream fermentation instead of upstream food degradation in the gut. Furthermore, this strain showed potential to colonize the gut and benefit the host via different strategies, such as short-chain fatty acids (SCFAs) generation by fermenting carbohydrates and amino acids, and the production of diverse vitamins and antibiotics to support the host growth and antipathogenicity. To our knowledge, strain B1 represents the first host-related pure culture ofPsychrilyobacter, acting as a potential probiotic gut anaerobe dominating in diverse marine invertebrates.ImportancePsychrilyobacteris a globally distributing bacterial genus and with an inhabiting preference for guts of marine invertebrates. Due to the difficulty of cultivation and the limited genomic information, its role in host remains largely unknown. We isolated the first host-associatedPsychrilyobacterspecies from abalone gut and uncovered its functional potentials to the host through different mechanisms. Our findings provide some insights into the understanding of host-microbe interactions on a core taxon with the marine invertebrates, and the isolate has an application prospect as a probiotic in protection of marine animals.

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Seasonal microbial profiling of left kidney and stomach of farmed adult greenlip abalone ( Haliotis laevigata )

Cited by 4 publications

References 51 publications

Monitoring Bacterial Community Dynamics in Abalone (Haliotis discus hannai) and the Correlations Associated with Aquatic Diseases

Monitoring Bacterial Community Dynamics in Abalone (Haliotis discus hannai) and the Correlations Associated with Aquatic Diseases

Genomic and metabolic insights into the first host-associated isolate of Psychrilyobacter

The first host-associated anaerobic isolate ofPsychrilyobacterprovides insights into its potential roles in the abalone gut

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