Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows

Zachut, Maya; Kra, Gitit; Livshitz, L.; Portnick, Y.; Yakoby, S.; Friedlander, Gilgi; Levin, Yishai

doi:10.1016/j.jprot.2017.02.011

Cited by 58 publications

(49 citation statements)

References 42 publications

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“…Agreeing with previous reports in humans and rodents (Jialal et al, 2015(Jialal et al, , 2016, we demonstrated an abundant expression of AHSG and FetA protein content in bovine preadipocytes and differentiated adipocytes. In agreement with Zachut et al (2017), our results also confirm that FetA is an adipokine in bovines. Additionally, our adipocyte culture model exhibited AHSG transcription levels similar to those observed in subcutaneous AT in early-lactation dairy cows, demonstrating that adipocyte culture models may be an efficient in vitro system for studying the effects of FetA on AT function.…”

Section: Feta Is An Adipokine and A Negative App In Bovine Adiposesupporting

confidence: 91%

“…Our in vitro data revealed a decrease in FetA protein content in adipocytes challenged with LPS and suggest that FetA is a negative APP in bovine AT. Similarly, FetA abundance in AT decreased 1.5 times during heat stress and was associated with increased proinflammatory and prooxidative status in late-pregnant dairy cows (Zachut et al, 2017). Currently, the direct effect of acute inflammation on FetA secretion by bovine AT remains poorly documented.…”

Section: Feta Is An Adipokine and A Negative App In Bovine Adiposementioning

confidence: 99%

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Fetuin-A: A negative acute-phase protein linked to adipose tissue function in periparturient dairy cows

Strieder‐Barboza

Souza

Raphael

et al. 2018

Journal of Dairy Science

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Fetuin-A (FetA) is a free fatty acid transporter and an acute-phase protein that enhances cellular lipid uptake and lipogenesis. In nonruminants, FetA is involved in lipid-induced inflammation. Despite FetA importance in lipid metabolism and inflammation, its expression and dynamics in adipose tissue (AT) of dairy cows are unknown. The objectives of this study were to (1) determine serum and AT FetA dynamics over the periparturient period and in mid-lactation cows in negative energy balance (NEB) after a feed restriction protocol and (2) characterize how an inflammatory challenge affects adipocyte FetA expression. Blood and subcutaneous AT were collected from 16 cows with high (≥3.75, n = 8) or moderate (≤3.5, n = 8) body condition score (BCS) at -26 ± 7 d (far off) and -8 ± 5 d (close up) before calving and at 10 ± 2 d after parturition (early lactation) and from 14 nonpregnant mid-lactation cows (>220 d in milk) after a feed restriction protocol. Serum FetA concentrations were 0.89 ± 0.13 mg/mL at far off, 0.96 ± 0.13 mg/mL at close up, and 0.77 ± 0.13 mg/mL at early lactation and were 1.09 ± 0.09 and 1.17 ± 0.09 mg/mL in feed-restricted and control cows, respectively. Serum and AT FetA contents decreased at the onset of lactation when lipolysis was higher. No changes in AT and serum FetA were observed after feed restriction induced NEB in mid-lactation cows. Prepartum BCS had no effect on serum FetA, but AT expression of AHSG, the gene encoding FetA, was reduced in periparturient cows with high BCS at dry-off throughout all time points. Circulating FetA was positively associated with serum albumin and calcium and with BCS variation over the periparturient period. The dynamics of AHSG expression were analogous to the patterns of lipogenic markers ABDH5, ELOVL6, FABP4, FASN, PPARγ, and SCD1. Expression of AHSG and FetA protein in AT was inversely correlated with AT proinflammatory markers CD68, CD44, SPP1, and CCL2. In vitro, bovine adipocytes challenged with lipopolysaccharide downregulated FetA protein expression. Adipocytes treated with FetA had lower CCL2 expression compared with those exposed to lipopolysaccharide. Overall, FetA is a systemic and local AT negative acute-phase protein linked to AT function in periparturient cows. Furthermore, FetA may support physiological adaptations to NEB in periparturient cows.

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Section: Feta Is An Adipokine and A Negative App In Bovine Adiposesupporting

confidence: 91%

Section: Feta Is An Adipokine and A Negative App In Bovine Adiposementioning

confidence: 99%

Fetuin-A: A negative acute-phase protein linked to adipose tissue function in periparturient dairy cows

Strieder‐Barboza

Souza

Raphael

et al. 2018

Journal of Dairy Science

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“…The transcription factor NFE2L2 represents a major mechanism for cells to defend against oxidative stress. Expression of the gene was first reported in the transition cow liver (Loor, 2010), and more recent research provided some evidence that it can play an important role during the transition period and late pregnancy (Gessner et al, 2013;Zachut et al, 2017). Therefore, it seems that NFE2L2 plays an important role in the adipose tissue adaptations to the onset of lactation in dairy cows.…”

Section: Abundance Of Proteins Associated With Gsh Metabolism and Nfe2l2mentioning

confidence: 98%

“…In nonruminants, nuclear factor erythroid 2-like 2 (NFE2L2; formerly Nrf2) is a key transcription factor controlling cellular oxidative stress in part by increasing the mRNA abundance of antioxidant enzymes (Kaspar et al, 2009). In dairy cows, heat stress was associated with greater protein abundance of NFE2L2 in subcutaneous adipose in late pregnancy, indicating that stressors can elicit a tissue-specific antioxidant response (Zachut et al, 2017). Thus, NFE2L2 may play an important role in the subcutaneous adipose tissue during the periparturient period.…”

mentioning

confidence: 99%

Glutathione metabolism and nuclear factor erythroid 2-like 2 (NFE2L2)-related proteins in adipose tissue are altered by supply of ethyl-cellulose rumen-protected methionine in peripartal Holstein cows

Liang

Batistel

Parys

et al. 2019

Journal of Dairy Science

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Enhancing the supply of rumen-protected Met (RPM) during the peripartum period alleviates inflammation and oxidative stress status in dairy cows. We tested the hypothesis that RPM could increase abundance of genes and proteins related to glutathione (GSH) metabolism and the antioxidant transcription factor nuclear factor erythroid 2-like 2 (NFE2L2) in subcutaneous adipose tissue. Multiparous Holstein cows were fed a basal diet [control prepartum diet = 1.47 Mcal/kg of dry matter (DM) and 15.3% crude protein; control postpartum diet = 1.67 Mcal/kg of DM and 17.7% crude protein] or the control plus ethylcellulose RPM at a rate of 0.09 and 0.10% of DM intake before expected calving and after calving, respectively. Sixty cows were assigned to treatments based on parity, previous 305-d milk yield, and body condition score at 28 d from parturition. Diets were fed from −28 to 30 d. Biopsies of subcutaneous adipose tissue collected on d −10, 10, and 30 relative to parturition from 7 cows in each group were used for measuring concentrations of GSH, reactive oxygen species, superoxide dismutase, malondialdehyde, and mRNA and protein abundance (Western blotting). A repeated-measures ANOVA was used for statistics. The statistical model included the random effect of block and fixed effects of treatment, time, and its interaction. There was a diet × time effect for reactive oxygen species due to lower concentrations in Met versus control cows specifically at d −10. Cows fed Met also had lower concentrations of malondialdehyde in subcutaneous adipose tissue. Compared with controls, overall mRNA abundance of the GSH metabolism-related genes cystathionine-β-synthase (CBS), glutamate-cysteine ligase modifier subunit (GCLM), glutathione reductase (GSR), and glutathione peroxi-dase 1 (GPX1) was greater in cows fed Met. Furthermore, supply of Met resulted in an overall upregulation of protein abundance of glutathione peroxidase (GPX) 1, GPX3, glutathione S-transferase mu 1 (GSTM1), and glutathione S-transferase α 4 (GSTA4), all related to GSH metabolism. There was a diet × time effect for protein abundance of NFE2L2 and its repressor Kelch-like ECH associated protein 1 (KEAP1) due to lower values at 30 d in cows fed Met versus controls. The abundance of phosphorylated NFE2L2 was lower at 30 d in response to Met. Overall, the data suggest that exogenous Met may play a role in activating GSH metabolism and the antioxidant NFE2L2 pathways in subcutaneous adipose tissue.

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“…Proteomic studies of heat stress effects have been mainly carried out on a number of plants and on animals such as dairy cows, beef cattle, pigs, and chickens. Proteins related to nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated oxidative stress were identi ed in dairy cows under summer heat stress, and several biomarkers (including mitogen-activated protein kinase kinase 1 (MAP2K1), glutathione S-transferase mu 1 (GSTM1), and stress-induced phosphoprotein 1 (STIP1)) were identi ed in adipose tissue in response to heat stress (8). In heat-stressed pigs, there was an increase in the expression of heat-shock proteins that protect cells by degrading denatured proteins; in ammatory cytokines, such as glucose-regulated protein 94 (GRP94) and Serpin family A member 3 (SERPINA3); and glutathione peroxidase and glutathione S-transferase, which are related to immune responses (9).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Protective Effect of Early Heat Exposure Against Chronic Heat Stress in Broilers

Kang

Shim

2020

Preprint

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BackgroundThe increasing trend of global warming has affected the livestock industry through the heat stress caused to the animals. Among them, poultry are the most susceptible to heat stress, which results in serious production problems. Therefore, a better understanding of the mechanisms related to the thermal reactions and thermal resistance in poultry would be helpful toward resolving the production issues. In this study, whole proteome analysis was carried out to identify differentially expressed proteins in the liver tissue of broilers under chronic heat stress (35℃/24 h, each day between the broiler ages of 21–35 days). Additionally, the effect of early heat exposure (40℃/24 h, for 1 day only on chicks at 5 days of age) was determined.ResultsIn total, 277 differentially expressed proteins due to chronic heat stress were identified (132 downregulated and 145 upregulated). Of those, 95 proteins were regulated by early heat exposure (42 downregulated and 53 upregulated during chronic heat stress). Of the 95 proteins, 8 were related to actin metabolism. According to the KEGG analysis, the proteins were mainly involved in pathways for carbon metabolism and carbohydrate metabolism. Under chronic heat stress, the proteins involved in carbohydrate metabolism were expressed in such a way as to promote the metabolism of carbohydrates, which is the natural means to reduce body temperature but may well induce cell damage.ConclusionEarly heat exposure reduced the heat stress-induced expression changes of select proteins, indicating the adaptability of the animal to chronic heat stress. The determination of the differentially expressed proteins in the liver proteome under chronic heat stress and early heat exposure suggests that the liver of broilers has various physiological mechanisms for regulating homeostasis to aid heat resistance.

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Seasonal heat stress affects adipose tissue proteome toward enrichment of the Nrf2-mediated oxidative stress response in late-pregnant dairy cows

Cited by 58 publications

References 42 publications

Fetuin-A: A negative acute-phase protein linked to adipose tissue function in periparturient dairy cows

Fetuin-A: A negative acute-phase protein linked to adipose tissue function in periparturient dairy cows

Glutathione metabolism and nuclear factor erythroid 2-like 2 (NFE2L2)-related proteins in adipose tissue are altered by supply of ethyl-cellulose rumen-protected methionine in peripartal Holstein cows

Proteomic Analysis of the Protective Effect of Early Heat Exposure Against Chronic Heat Stress in Broilers

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