Seasonal effect on tissue culture response and plant regeneration frequency from non-bombarded and bombarded immature scutella of barley (Hordeum vulgare) harvested from controlled environment

Sharma, Vidya; Hänsch, Robert; Mendel, Ralf R.; Schulze, Jutta

doi:10.1007/s11240-004-2617-9

Cited by 20 publications

(15 citation statements)

References 36 publications

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“…Histological studies have identified the scutellum of an immature embryo as the tissue from which embryogenic calli originate [17][18][19][20]. Few studies, however, have examined the anatomy of somatic embryogenesis and the ontogeny of somatic embryos obtained from scutella [1,11,[21][22][23].…”

Section: Introductionmentioning

confidence: 99%

The effect of genotype on a barley scutella culture. Histological aspects

et al. 2013

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Cereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).

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Section: Introductionmentioning

confidence: 99%

The effect of genotype on a barley scutella culture. Histological aspects

et al. 2013

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“…Fuentes et al (2000) reported that specificity of carbohydrates may have differential effects on morphogenesis. Even though it is well documented that sucrose is the best source of carbon for in vitro systems, there are also some reports where maltose was found to give better results than other carbohydrates (Ainsley and Aryan, 1998;Chang et al, 2003;Sharma et al, 2005).…”

Section: Effect Of Carbon Sources To Callus Induction and Regenerationmentioning

confidence: 99%

Factors influencing callus induction and plant regeneration of Dahurian wildrye grass (Elymus dahuricus L.)

Lee#¹,

Chinzorig²,

Choi³

et al. 2012

Afr. J. Biotechnol.

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“…Salome) were grown under environmentally controlled conditions in the greenhouse as described (Sharma et al, 2005). Caryopses were harvested and sterilized (Ryschka et al, 1991) and immature scutella were isolated as reported .…”

Section: Plant Material Transformation and Generation Of Transgenic mentioning

confidence: 99%

“…Transformation was performed with all three constructs using the BIOLISTICä particle delivery system according to the procedure described earlier . Transformed scutella were selected on MS-based medium (Murashige and Skoog, 1962) containing 3 mg/l bialaphos (Duchefa, Haarlem, The Netherlands) and putative transgenic plants were generated as described recently (Sharma et al, 2005). A continuous supply of material enabled two independent transformation experiments per month each including 250-350 scutella.…”

Section: Plant Material Transformation and Generation Of Transgenic mentioning

confidence: 99%

Transgenic barley plants overexpressing a 13-lipoxygenase to modify oxylipin signature

Sharma

Monostori

Göbel³

et al. 2006

Phytochemistry

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Three chimeric gene constructs were designed comprising the full length cDNA of a lipoxygenase (LOX) from barley (LOX2:Hv:1) including its chloroplast targeting sequence (cTP) under control of either (1) CaMV35S-or (2) polyubiquitin-1-promoter, whereas the third plasmid contains 35S promoter and the cDNA without cTP. Transgenic barley plants overexpressing LOX2:Hv:1 were generated by biolistics of scutella from immature embryos. Transformation frequency for 35S::LOX with or without cTP was in a range known for barley particle bombardment, whereas for Ubi::cTP-LOX no transgenic plants were detected. In general, a high number of green plantlets selected on bialaphos became yellow and finally died either in vitro or after potting. All transgenic plants obtained were phenotypically indistinguishable from wild type plants and all of them set seeds. The corresponding protein (LOX-100) in transgenic T0 and T1 plants accumulated constitutively to similar levels as in the jasmonic acid methyl ester (JAME)-treated wild type plants. Moreover, LOX-100 was clearly detectable immunocytochemically within the chloroplasts of untreated T0 plants containing the LOX-100-cDNA with the chloroplast target sequence. In contrast, an exclusive localization of LOX-100 in the cytoplasm was detectable when the target sequence was removed. In comparison to sorbitol-treated wild type leaves, analysis of oxylipin profiles in T2 progenies showed higher levels of jasmonic acid (JA) for those lines that displayed elevated levels of LOX-100 in the chloroplasts and for those lines that harboured LOX-100 in the cytoplasm, respectively. The studies demonstrate for the first time the constitutive overexpression of a cDNA coding for a 13-LOX in a monocotyledonous species and indicate a link between the occurrence of LOX-100 and senescence.

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Seasonal effect on tissue culture response and plant regeneration frequency from non-bombarded and bombarded immature scutella of barley (Hordeum vulgare) harvested from controlled environment

Cited by 20 publications

References 36 publications

The effect of genotype on a barley scutella culture. Histological aspects

The effect of genotype on a barley scutella culture. Histological aspects

Factors influencing callus induction and plant regeneration of Dahurian wildrye grass (Elymus dahuricus L.)

Transgenic barley plants overexpressing a 13-lipoxygenase to modify oxylipin signature

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