Seasonal dynamics of the structure of epiphytic yeast communities

Glushakova, A. M.; Chernov, I. Yu.

doi:10.1134/s0026261710060160

Cited by 65 publications

(22 citation statements)

References 9 publications

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“…Another aspect previously documented [22,24,26] and supported by our studies, concerns the marked differences obtained in different seasons and geographic locations. It is relevant to note that in our study we have collected corn leaf samples in different growth stages and growing conditions of the plants, which may have affected the results.…”

Section: Discussionsupporting

confidence: 85%

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

et al. 2020

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The ecology and diversity of phylloplane yeasts is less well understood in tropical regions than in temperate ones. Therefore, we investigated the yeast diversity associated with the phylloplane of corn, an economically important crop in Thailand, by a culture-dependent method. Thirty-six leaf samples were collected and 217 yeast strains were isolated by plating leaf-washings. The strains were grouped by PCR-fingerprinting and representative strains were identified by analysis of the D1/D2 domain of the large subunit rRNA gene. In total, 212 strains were identified within 10 species in the Ascomycota and 32 species in the Basidiomycota. Five strains represented potential new species in the Basidiomycota, one strain was recently described as Papiliotrema plantarum, and four strains belonged to the genera Vishniacozyma and Rhodotorula. A higher number of strains in the Basidiomycota (81.6%) was obtained. Hannaella sinensis was the species with the highest occurrence. Principal coordinates analysis ordinations of yeast communities revealed that there were no differences in the similarity of the sampling sites. The estimation of the expected species richness showed that the observed species richness was lower than expected. This work indicated that a majority of yeast associated with the phylloplane of corn plant belongs to the phylum Basidiomycota.

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Section: Discussionsupporting

confidence: 85%

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

et al. 2020

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“…It should also be noted that R. collo‐cygni was not only found in its primary barley host, but also in small amounts in the other investigated crops, which may add to the epidemiological risk of the pathogen, as also discussed by Sachs (). Most yeasts were found across the range of host species, which is in agreement with other studies (Inacio et al ., ; Glushakova & Chernov, ). However, a small number of species exhibited significantly different abundances across host species, which may be caused by subtle differences in nutrient availability or leaf surface topology of the hosts.…”

Section: Discussionmentioning

confidence: 99%

Host genotype is an important determinant of the cereal phyllosphere mycobiome

et al. 2015

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SummaryThe phyllosphere mycobiome in cereals is an important determinant of crop health. However, an understanding of the factors shaping this community is lacking.Fungal diversity in leaves from a range of cultivars of winter wheat (Triticum aestivum), winter and spring barley (Hordeum vulgare) and a smaller number of samples from oat (Avena sativa), rye (Secale cereale) and triticale (Triticum 9 Secale) was studied using next-generation sequencing. The effects of host genotype, fungicide treatment and location on fungal communities were explored.In total, 635 251 fungal internal transcribed spacer (ITS) reads were obtained from 210 leaf samples. Visual disease assessments and relative read abundance of Zymoseptoria tritici and Ramularia collo-cygni were strongly positively related. Crop genotype at the species level explained 43% of the variance in the total dataset, followed by fungicide treatment (13%) and location (4%). Indicator species, including plant pathogens, responding to factors such as crop species, location and treatment were identified.Host genotype at both the species and cultivar level is important in shaping phyllosphere fungal communities, whereas fungicide treatment and location have minor effects. We found many host-specific fungal pathogens, but also a large diversity of fungi that were relatively insensitive to host genetic background, indicating that host-specific pathogens live in a 'sea' of nonspecific fungi.

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“…Fruits and vegetables intended for storage are the living vegetative and reproductive organs of plants, which surface is abun- dantly inhabited by microorganisms that were given the name «epiphytes» [16,17]. Recently, more and more information have appeared showing that plant internal tissues are also inhabited by microorganisms called endophytes [18,19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

An Effect of Storage and Transportation Temperature on Quantitative and Qualitative Composition of Microflora of Plant Products

et al. 2019

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The results of the study on changes in the composition and quantity of epiphytic and endophytic microorganisms of plant products during storage and transportation are presented. For the investigation, the authors took apple fruits and leafy spicy green products that had biological peculiarities and allowed investigating processes of the long-term and short-term main stages (cold storage, transportation by refrigerated transport, presales storage) of the continuous cold chain on the way to a consumer. Apple fruits were placed in storage in cold chambers with the temperature regimes of plus (2–3)°C and minus (1–2)°C, where they were stored for 90 days. The vegetative organs of dill and parsley were transported during 8 hours by a refrigerated truck and placed in the commercial refrigeration equipment at two temperature regimes (4–5) C and (0–1) C for 72 hours for presales storage. The results of the microbiological analysis showed that the number of endophytic microorganisms (bacteria, yeasts and molds) was lower by 1–3 orders of magnitude in apple fruits and by 2–3 times in green vegetables compared to the number of epiphytic microorganisms. It was established that the regime of storage at negative temperatures completely inhibited the development of epiphytic bacteria on fruits, significantly delayed the multiplication of epiphytic yeasts and molds; while at a positive temperature the number of bacteria increased approximately by 10–17 times, yeasts by 180 times and molds by 3 times. The dynamics of changes in the number of endophytic microorganisms during storage showed the same trend that was observed for epiphytic microorganisms. Analysis of the microbial quantity after transportation of green products showed an increase in abundance of the revealed groups of epiphytes and endophytes by 1.5–3 times upon absolute prevalence of bacteria. After short-term storage, a significant growth of the revealed microbial groups was found; with that, their quantity was 1.5–6.5 times higher at (4–5) C than at (0–1) C. The authors experimentally confirmed the conclusion that with respect to reduction of losses due to microbiological spoilage and extension of shelf life, the cold storage regime of the studied plant products at near-zero temperatures is preferable compared to the regimes of storage at higher positive temperatures.

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Seasonal dynamics of the structure of epiphytic yeast communities

Cited by 65 publications

References 9 publications

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

Host genotype is an important determinant of the cereal phyllosphere mycobiome

An Effect of Storage and Transportation Temperature on Quantitative and Qualitative Composition of Microflora of Plant Products

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