Seasonal differences of lysosomal, lipid and lipofuscin parameters in the digestive gland of the mussel Mytilus galloprovincialis

Koukouzika, N.; Raftopoulou, E.K.; Dimitriadis, V.K.

doi:10.1093/mollus/eyp022

Cited by 27 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2c,d,3b,c). The marked seasonal variability of neutral lipid content has previously been reported for M. galloprovincialis by other authors and has been related to both reproductive cycle and food supply (Cancio et al 1999;Koukouzika et al 2009;Nesto et al 2007). M. galloprovincialis may be considered a "conservative species", with periods of reserve accumulation and gamete production temporally separated (Bayne 1976).…”

Section: Temporal Variability Of the Biological Responsessupporting

confidence: 65%

Biomonitoring approach with mussel Mytilus galloprovincialis (Lmk) and clam Ruditapes philippinarum (Adams and Reeve, 1850) in the Lagoon of Venice

Moschino

Delaney

Meneghetti

et al. 2010

Environ Monit Assess

View full text Add to dashboard Cite

Transplanted Mytilus galloprovincialis and native Ruditapes philippinarum were deployed in 10 sampling stations with different pollution impact within the Lagoon of Venice to evaluate the temporal variations and the suitability of the following cytochemical and histochemical biomarkers just as indicators of environmental stress: lysosomal membrane stability, lipofuscins, neutral lipids and lysosome to cytoplasm volume ratio. The physiological status of the organisms was also investigated by determining the survival in air capability and the reburrowing rate (clams). The biological parameters were assessed in June and October. Furthermore, for a better definition of the environmental aspects of the study sites, heavy metal, PAH and PCB concentrations were also evaluated in the sediments. As a whole, the biological responses examined in both species from all the sampling sites showed significant differences between the two seasonal campaigns, only lysosomal membrane stability exhibited less variability. Pollutants in sediments generally showed low-intermediate contamination levels, few hotspots persisting mostly in the inner areas of the lagoon, the most influenced by the industrial zone. Transplanted mussels were more responsive than native clams and the biological responses of both species varied temporally. The range of the spatial variability was always narrow and reflected only partially the broader variability shown by the chemical content in the sediments. In this sense, biological responses seemed to be particularly influenced by the high temporal and spatial heterogeneity that characterise the Lagoon of Venice, as well as most of the transitional environments.

show abstract

Section: Temporal Variability Of the Biological Responsessupporting

confidence: 65%

Biomonitoring approach with mussel Mytilus galloprovincialis (Lmk) and clam Ruditapes philippinarum (Adams and Reeve, 1850) in the Lagoon of Venice

Moschino

Delaney

Meneghetti

et al. 2010

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…Mussels were observed to be actively feeding following addition of microalgae and exposure to PS-MP, PA-MF and PS-NP. Food ingested by mussels is physically homogenised by the rotating crystalline style, digested extracellularly in the stomach, and then shunted into the digestive gland to undergo intracellular digestion (Gosling, 2003;Koukouzika et al, 2009); the glands comprise terminal epithelial tubules, with undigested food returned to the stomach prior to egestion, and as such may be considered a "dead-end" in which particulates might be retained (Faggio et al, 2018). Both PS-MP and PA-MF were identified in the digestive glands of mussels, with a significantly greater amount of microplastics identified in digestive glands following the 7-day exposure (two-way ANOVA, Time: P<0.05; Figure 1).…”

Section: Microplastic and Nanoplastic Uptakementioning

confidence: 99%

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Cole

Liddle

Consolandi

et al. 2020

Marine Pollution Bulletin

152

View full text Add to dashboard Cite

We compare the toxicity of microplastics, microfibres and nanoplastics on mussels. Mussels (Mytilus spp.) were exposed to 500 ng mL-1 of 20 µm polystyrene microplastics, 10x30 µm polyamide microfibres or 50 nm polystyrene nanoplastics for 24 h or 7 days. Biomarkers of immune response, oxidative stress response, lysosomal destabilisation and genotoxic damage were measured in haemolymph, digestive gland and gills. Microplastics and microfibres were observed in the digestive glands, with significantly higher plastic concentrations after 7-days exposure (ANOVA, P<0.05). Nanoplastics had a significant effect on hyalinocyte-granulocyte ratios (ANOVA, P<0.05), indicative of a heightened immune response. SOD activity was significantly increased followed 24 h exposure to plastics (two-way ANOVA, P<0.05), but returned to normal levels after 7-days exposure. No evidence of lysosomal destabilisation or genotoxic damage was observed from any form of plastic. The study highlights how particle size is a key factor in plastic particulate toxicity.

show abstract

“…These reserves are used for basic metabolism in months when food is scarce, but also for gametogenesis [42]. Digestive gland is the main site for lipid reserves both in scallops and mussels, where the main type of lipids consists of neutral lipids [44]. The Adriatic P. jacobaeus levels of GLU and TRIG were the highest in fall, CHOL in summer (gonads) and winter (digestive gland).…”

Section: Plos Onementioning

confidence: 99%

Seasonal antioxidant and biochemical properties of the Northern Adriatic Pecten jacobaeus

Popović¹,

Ljubić²,

Strunjak‐Perović³

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Seasonal differences of lysosomal, lipid and lipofuscin parameters in the digestive gland of the mussel Mytilus galloprovincialis

Cited by 27 publications

References 26 publications

Biomonitoring approach with mussel Mytilus galloprovincialis (Lmk) and clam Ruditapes philippinarum (Adams and Reeve, 1850) in the Lagoon of Venice

Biomonitoring approach with mussel Mytilus galloprovincialis (Lmk) and clam Ruditapes philippinarum (Adams and Reeve, 1850) in the Lagoon of Venice

Microplastics, microfibres and nanoplastics cause variable sub-lethal responses in mussels (Mytilus spp.)

Seasonal antioxidant and biochemical properties of the Northern Adriatic Pecten jacobaeus

Contact Info

Product

Resources

About