Seasonal Depth-Related Gradients in Virioplankton: Standing Stock and Relationships with Microbial Communities in Lake Pavin (France)

Colombet, Jonathan; Charpin, Marie; Robin, Agnès; Portelli, Christophe; Amblard, Cécile; Cauchie, Henry‐Michel; Sime-Ngando, Télesphore

doi:10.1007/s00248-009-9535-7

Cited by 17 publications

(16 citation statements)

References 47 publications

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“…This corroborates the hypothesis that this layer is a key transitive microhabitat in Lake Pavin, where the complex mixolimnic biological communities and the related interactions are replaced by a simple viral loop system [i.e. dissolved organic matter (DOM) > prokaryote > viruses > DOM] towards the dark and permanently anoxic monimolimnion [20,21]. In general, the oxycline/chemocline layer in Lake Pavin is well known as an important barrier, characterized by particular geochemical properties, that acts as a key interface in the vertical flow and recycling of matter through the water column [1].…”

Section: Empirical Forcing Of Depth-related Gradients In Viral Lytic supporting

confidence: 80%

“…In this paper, viruses were correlated mainly with microautotrophs in the mixolimnion but exclusively with heterotrophic prokaryotes in the monimolimnion where the other microbial communities were apparently constrained by the dark anoxic conditions [21]. In the present paper and based on the same type of analyses, the differential responses of lytic viruses to the depth-related gradients were confirmed.…”

Section: Empirical Forcing Of Depth-related Gradients In Viral Lytic supporting

confidence: 72%

“…heterotrophic bacteria, picocyanobacteria, autotrophic picoeukaryotes and heterotrophic and autotrophic nanoflagellates), have been described and discussed in detail in a companion paper [21].…”

Section: Other Variablesmentioning

confidence: 98%

“…The deep, meromictic and dimictic Lake Pavin exhibits a typical vertical succession of physico-chemical conditions [1], offering a unique model to study virus-protozoa-bacteria interactions in relation to physico-chemical and biological depth-related gradients. This study complements a companion paper in which the standing stocks of viruses and microbial communities are examined [21].…”

Section: Introductionmentioning

confidence: 95%

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Seasonal Depth-Related Gradients in Virioplankton: Lytic Activity and Comparison with Protistan Grazing Potential in Lake Pavin (France)

Colombet

Sime-Ngando

2012

Microb Ecol

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This study presents an original depth-related survey of virioplankton lytic activity in relation to prokaryotic production and potential protistan bacterivory in the deep (Z(max) = 92 m) meromictic volcanic Lake Pavin (Massif Central, France). The sampling strategy was designed to be representative of the physico-chemical gradients of the water column of the lake, and of the seasonal variability as well, i.e. 12 different depths sampled in triplicates from April to December 2005. In the space, viral lytic activity estimated from the frequency of visibly infected prokaryotic cells and from burst size over the study period generally decreased with depth. This was viewed as a paradox compared to the abundances of viruses and prokaryotes and to the prokaryotic production which increased with depth. The seasonal variability in viral lytic activity was correlated with prokaryotic variables (abundance and production) in the deepest waters, i.e. from the hypolimnion downwards. Compared to previous studies known from the mixolimnion, we conclude that the deep waters in Lake Pavin represent an exclusive environment for heterotrophic prokaryotes whose seasonal activity offers an optimal and unique resource for thriving viral communities, some of which may be typical, endemic to the ambient dark, cold and stable deep water masses. Overall, the main findings in the present study get well around a previous statement that the ecology of the deepest waters of Lake Pavin is essentially driven by the dark viral loop (dissolved organic matter-prokaryotes-viruses) processes, which can sequester organic matters and nutrients for a long-lived turnover time. This is in agreement with recent demonstrations from marine systems that meso- and bathypelagic waters are optimal environments for viral survival and proliferation.

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Section: Empirical Forcing Of Depth-related Gradients In Viral Lytic supporting

confidence: 80%

Section: Empirical Forcing Of Depth-related Gradients In Viral Lytic supporting

confidence: 72%

Section: Other Variablesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 95%

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Seasonal Depth-Related Gradients in Virioplankton: Lytic Activity and Comparison with Protistan Grazing Potential in Lake Pavin (France)

Colombet

Sime-Ngando

2012

Microb Ecol

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“…Colombet, et al [17] proposed using PEG (polyethylene glycol) precipitation as an alternative to density gradient ultracentrifugation for purifying viruses. Concentrating bacteriophages by ultrafiltration is a further alternative to ultracentrifugation and has several advantages, including the removal of proteins that could cause bacterial lysis [18,19].…”

Section: Phage Lysate Preparationmentioning

confidence: 99%

Potentiometric Investigation of the Dynamics of Bacteriophage - Induced Potassium Ion Efflux in Escherichia coli

Galgalkar¹

2016

Ely J Micro

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Potassium is a very stable component of biological systems and contributes to maintaining positive turgor pressure of the cells. Although ion-selective electrodes (ISEs) have been used previously for measuring bacteriophage -induced potassium ion efflux in bacteria, the dynamics of this outcome remain unclear. In this study, the effects of incubation time, reaction volume, and phage/bacterial concentration on bacteriophage-induced potassium ion efflux were studied using a commercial ISE. A phage-bacteria reaction volume of 200 µl incubated at 37°C for 30 minutes was found to be optimal for detecting potassium ion efflux using the ISE. Potassium ion efflux was found to be directly proportional to phage multiplicity of infection and ~5 × 10 7 cfu/ml appeared to be the lowest bacterial concentration at which potassium ion efflux could be measured reliably. Furthermore, the differences in potassium ion efflux between control (bacteria + assay buffer) and test (bacteria + phage) samples were statistically significant (p < 0.05) and the results were consistent across assays performed on different days. The average of absolute concentrations of effluxed potassium ions was 1.4 mg/L for ~5 × 10 8 cfu/ml of bacteria (MOI (Multiplicity of infection) = 100). The method uses simple bench-top technology, has a rapid turnaround time, is label-free, and has the potential for incorporation in bacterial detection systems.

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Lake Pavin: A Pioneer Site for Ecological Studies of Freshwater Viruses

Sime-Ngando¹,

Bettarel²,

Colombet³

et al. 2016

Lake Pavin

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Seasonal Depth-Related Gradients in Virioplankton: Standing Stock and Relationships with Microbial Communities in Lake Pavin (France)

Cited by 17 publications

References 47 publications

Seasonal Depth-Related Gradients in Virioplankton: Lytic Activity and Comparison with Protistan Grazing Potential in Lake Pavin (France)

Seasonal Depth-Related Gradients in Virioplankton: Lytic Activity and Comparison with Protistan Grazing Potential in Lake Pavin (France)

Potentiometric Investigation of the Dynamics of Bacteriophage - Induced Potassium Ion Efflux in Escherichia coli

Lake Pavin: A Pioneer Site for Ecological Studies of Freshwater Viruses

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