Seasonal Colonization of Roots of Field-Grown Cotton by<i>Verticillium dahliae</i>and<i>V. tricorpus</i>

Huisman, O. C.

doi:10.1094/phyto-78-708

Cited by 46 publications

(32 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While Huisman (30) estimated an average colony size of only 2.3 mm by analyzing the recovery of V. dahliae from cotton root segments, later studies using immunoenzymatic staining of V. dahliae increased this estimate to 7.3 mm and found an increase in colony length correlated with increased distance from the root apex (22). Despite the distinctly different methods employed between this study and Huisman (30) and Gerik and Huisman (22), the observations are nearly similar. In cotton, cortical root infections occur 1,000-fold in excess of systemic infections (29).…”

Section: Discussionsupporting

confidence: 50%

“…Numerous researchers reported sudden increases in V. dahliae soil populations during the production of a crop (24,30,36,38), and either attributed this increase to sporulation (24,36,38) following the germination of microsclerotia, or to the release of microsclerotia from the breakdown of residues from a previous crop (30). Early sporulation on infected rootlets is also a possibility.…”

Section: Discussionmentioning

confidence: 99%

“…Besides the noticeable collapse of the root tip, no symptoms were evident beyond the infection site as the mycelia advanced systemically through xylem vessels towards the taproot. Huisman (30) reported that the number of V. dahliae colonies per root length remained constant and randomly distributed in direct relation to soil inoculum levels initially, but subsequently an increase in the number of colonies per root length and a deviation from a random distribution occurred. In lettuce, initial colonies also appeared randomly distributed as conidia germinated following the root-dip or soil drench methods.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Vallad

Subbarao²

2008

Phytopathology®

143

109

View full text Add to dashboard Cite

Interactions between lettuce and a green fluorescent protein (GFP)-expressing, race 1 isolate of Verticillium dahliae, were studied to determine infection and colonization of lettuce cultivars resistant and susceptible to Verticillium wilt. The roots of lettuce seedlings were inoculated with a conidial suspension of the GFP-expressing isolate. Colonization was studied with the aid of laser scanning confocal and epi-fluorescence microscopes. Few differences in the initial infection and colonization of lateral roots were observed between resistant and susceptible cultivars. Hyphal colonies formed on root tips and within the root elongation zones by 5 days, leading to the colonization of cortical tissues and penetration of vascular elements regardless of the lettuce cultivar by 2 weeks. By 8 to 10 weeks after inoculation, vascular discoloration developed within the taproot and crown regions of susceptible cultivars well in advance of V. dahliae colonization. Actual foliar wilt coincided with the colonization of the taproot and crown areas and the eruption of mycelia into surrounding cortical tissues. Advance colonization of stems, pedicels, and inflorescence, including developing capitula and mature achenes was observed. Seedborne infection was limited to the maternal tissues of the achene, including the pappus, pericarp, integument, and endosperm; but the embryo was never compromised. Resistant lettuce cultivars remained free of disease symptoms. Furthermore, V. dahliae colonization never progressed beyond infected lateral roots of resistant cultivars. Results indicated that resistance in lettuce may lie with the plant's ability to shed infected lateral roots or to inhibit the systemic progress of the fungus through vascular tissues into the taproot.

show abstract

Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Vallad

Subbarao²

2008

Phytopathology®

143

109

View full text Add to dashboard Cite

show abstract

“…These observations in part confirm those of Davis & McDole (1979) and Davis et al (2000) on the pectate based agar NPX (Butterfield & DeVay 1977) and of Huisman (1988) on a similar pectate medium. All authors mentioned the radially grouped V. dahliae, and scattered, dark hyphae bearing V. tricorpus microsclerotia, as well as the microsclerotial size difference.…”

Section: Discussionsupporting

confidence: 88%

Morphology of Verticillium dahliae and V. tricorpus on semi-selective media used for the detection of V. dahliae in soil

Goud

Termorshuizen

Gams

2003

Mycological Research

View full text Add to dashboard Cite

“…The material recovered on the lower sieve (32 lm) was collected in a flask and re-suspended in 100 ml of sterile distilled water, then aliquots of 2 ml were distributed in Petri dishes containing the V. dahliae semi-selective medium reported by Huisman (1988). After 15 days of incubation at 24°C in the dark, plates were washed under a gentle flow of tap water and then incubated for an additional time of 15 days at the same temperature.…”

Section: Monitoring Of V Dahliae Ms In the Rhizospherementioning

confidence: 99%

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

Vitullo

Altieri

Esposito

et al. 2012

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Two organic amendments (OMW-M1 and OMW-M2), based on olive mill wastes (OMWs) subjected to advanced processes of aerobic static storage or composting, were tested for their suppressive activity against Verticillium dahliae, the causal agent of olive Verticillium wilt. OMW-M1 and OMW-M2 drastically inhibited the pathogen growth in vitro and then were further tested in suppressive pot experiments. The amendments, mixed at 15 % (v/v) with a nursery standard plant-growth matrix, were tested alone or in combination with two biocontrol bacteria (Bacillus amyloliquefaciens and Burkholderia cepacia) selected from suppressive soils. All mixtures were artificially contaminated with V. dahliae microsclerotia (MS), the density of which was periodically monitored by either a semi-selective medium or a specific real-time Polymerase Chain Reaction technique. In plant-less pot assays conducted in a growth chamber, OMW-M1 was the most effective amendment, reducing V. dahliae MS density by 100 % after 90 days with respect to the untreated control. In nursery experiments with pot-growing olive plants, OMW-M1, particularly when combined with the biocontrol bacteria, confirmed its strong suppressive activity reducing up to 100 % the density of V. dahliae MS in the rhizosphere behaving even better than a commercial biofungicide (Trichoderma asperellum TV1) used as a control. The best combined treatment also reduced plant mortality and increased root and shoot extension. It is concluded that organic amendments from stabilized olive mill by-products showed positive agronomic and phytosanitary properties on pot-growing olive plants and, particularly when enriched with biocontrol agents, they are potentially suitable for use in sustainable agriculture.

show abstract

Seasonal Colonization of Roots of Field-Grown Cotton byVerticillium dahliaeandV. tricorpus

Cited by 46 publications

References 0 publications

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Colonization of Resistant and Susceptible Lettuce Cultivars by a Green Fluorescent Protein-Tagged Isolate of Verticillium dahliae

Morphology of Verticillium dahliae and V. tricorpus on semi-selective media used for the detection of V. dahliae in soil

Suppressive biomasses and antagonist bacteria for an eco-compatible control of Verticillium dahliae on nursery-grown olive plants

Contact Info

Product

Resources

About