Seasonal Changes of Nitrogen Storage Compounds in a Rhizomatous Grass Calamagrostis epigeios

Gloser, Vít

doi:10.1023/a:1022329210127

Cited by 26 publications

(18 citation statements)

References 19 publications

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“…The metabolomic and transcriptomic data support the hypothesis that the build-up of amino acids in the leaves of S. stapfianus during drying results from the mobilization of nitrogen from the mature DS leaves that senesce during this process, as proposed by Martinelli et al, 2007 [15]. This might be a survival feature derived from the perennial aspects of nitrogen storage and remobilization during overwintering commonly seen in C 4 forage grasses [16]. This would allow S. stapfianus to make efficient use of assimilated nitrogen during rehydration and is perhaps a critical need in its native nutrient-poor habitat [98].…”

Section: Discussionsupporting

confidence: 68%

“…The transcriptome of the leaves undergoing dehydration appears to support this hypothesis as the abundance of transcripts encoding amino acid biosynthetic enzymes was generally unaltered (Additional file 1: Table S1), whereas that of transcripts encoding enzymes involved in protein degradation (Additional file 6: Table S6i) are elevated and within the collection of SDATs. Storing nitrogen in the form of amino acids in the roots and crown tissues under stress conditions is a common adaptive tool that perennial grasses use to support new growth when conditions become favorable [16]. Thus, it appears that S. stapfianus sequesters amino acids in the younger desiccation-tolerant leaves rather than in the roots and crown tissues as part of its strategy for surviving desiccation.…”

Section: Discussionmentioning

confidence: 99%

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Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

et al. 2017

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BackgroundUnderstanding the response of resurrection angiosperms to dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses.MethodsWe have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration.ResultsThe rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus.ConclusionsThe transcriptomes of both dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of dehydration, desiccation, and rehydration.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1013-7) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

et al. 2017

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“…Reduction in amino-N concentrations of roots and rhizomes of N-deprived (0–0 and 150–0 N treatments) plants suggest this pool is an important contributor of N to shoot growth in spring. Gloser (2002 , 2005 ) also reported extensive loss of amino acids from rhizomes of C. epigejos in spring followed by a gradual increase in this N pool from July through November. He concluded that amino acids in rhizomes, roots and stem bases have a central role in N storage, winter survival, and spring growth of this species.…”

Section: Discussionmentioning

confidence: 89%

Nitrogen Reserve Pools in Two Miscanthus × giganteus Genotypes under Contrasting N Managements

Dierking¹,

Allen

Cunningham

et al. 2017

Front. Plant Sci.

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Nitrogen (N) reserves in vegetative tissues contribute N to regrowth of Miscanthus × giganteus shoots in spring, but our understanding of how N fertilization and plant genotype affect this process is incomplete. Our specific objectives were to: (1) determine how N fertilizer management impacts accumulation of dry matter and N among aboveground and belowground tissues and organs; (2) understand how changes in N management and tissue N concentration influence seasonal fluctuations in concentrations of buffer-soluble proteins and amino acids in putative storage organs including rhizomes and roots; and (3) characterize genotypic variability and genotype × N interactions for N reserve accumulation and use among Miscanthus × giganteus genotypes. Established plots of the IL Clone and Nagara-sib population were fertilized with 0–0, 0–150, 75–75, 150–0, and 150–150 kg N ha-1 where the first numeral denotes the N rate applied in 2011 (Year 1) and the second number denotes the N rate applied in 2012 (Year 2). Rhizomes, roots, stembases, and shoots were sampled at 6-week intervals between March and August and then in November at dormancy. Concentrations of N, soluble protein and amino-N increased in all tissues with fertilizer N application. With the exception of rhizome amino-N, concentrations of these N pools in roots and rhizomes declined as plants resumed growth in spring and increased sharply between August and November as growth slowed. Losses in shoot and stembase N mass between August and November were similar to total N accumulation in roots and rhizomes during this interval. Compared to the unfertilized control, specific N managements enhanced growth of above- and belowground tissues. The IL Clone generally had greater biomass yield of all organs than the Nagara-sib; the exception being shoot biomass in November when extensive leaf senescence reduce yield of the IL Clone. High biomass yields were obtained with 75 kg N ha-1 applied annually rather than semi-annual N applications of 150 kg N-1 ha that depended on N recycling from roots/rhizomes as a supplemental N source.

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“…Adaptability to different habitats and competitive strength arise from its ability to create different ecotypes (Jańczyk-Węglarska, 1996). Another important feature of C. epigejos in this context is efficient nitrogen uptake from the soil, high accumulation of this element in the roots and shoot bases, and the capacity for its effective translocation from aerial shoots before their dieback to underground storage organs (Gloser, 2002(Gloser, , 2005Gloser et al, 2004;Kavanová and Gloser, 2005). That efficient nitrogen usage allows C. epigejos to grow very fast and to produce a large biomass.…”

Section: Introductionmentioning

confidence: 99%

Habitat factors influencing the competitive ability ofCalamagrostis epigejos (L.) Roth in mountain plant communities

Pruchniewicz¹,

Żołnierz²,

Andonovski³

2017

Turk J Bot

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The aim of this study was to assess how the expansion of Calamagrostis epigejos influences the species composition and diversity of forest, clearing, and meadow communities as well as how habitat factors influence biometric variability and features determining the potential of Calamagrostis epigejos for expansion in the studied communities. The study was conducted in the central part of the Sudetes Mountains (SW Poland). Four types of communities with C. epigejos occurrence were chosen: a beech forest (Luzulo luzuloidis-Fagetum), a Norway spruce monoculture, a clearing community belonging to the Epilobietea angustifolii class, and a mesic mountain meadow of the Arrhenatheretalia order. In each of the studied communities, randomized selection of 10 plots (1 × 1 m) was performed. On these plots relevés were sampled, a biometric study was conducted, and soil samples were collected. The research revealed a significant influence of C. epigejos expansion on the species composition, diversity, and productivity in the herb layers of the studied communities. It also indicated a strong positive influence of sunlight availability and studied physicochemical properties of soil on the expansion ability of C. epigejos.

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Seasonal Changes of Nitrogen Storage Compounds in a Rhizomatous Grass Calamagrostis epigeios

Cited by 26 publications

References 19 publications

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

Sporobolus stapfianus: Insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics

Nitrogen Reserve Pools in Two Miscanthus × giganteus Genotypes under Contrasting N Managements

Habitat factors influencing the competitive ability ofCalamagrostis epigejos (L.) Roth in mountain plant communities

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