Seasonal changes of basic erythrocyte-metric parameters in Pelophylaxridibundus (Amphibia: Ranidae) from anthropogenically polluted biotopes in Southern Bulgaria and their role as bioindicators

Zhelev, Zhivko M.; Mehterov, Nikolay; Popgeorgiev, Georgi

doi:10.1016/j.ecoenv.2015.11.011

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…Similarly, Pelophylax ridibundus (Pallas, 1771) associated with low concentrations of Fe, Mn, Cu and Pb reported low lymphocytes, basophils, eosinophils and segmented nuclei neutrophils, while the number of monocytes increased (Zhelev et al, 2013b(Zhelev et al, , 2014b. Parallel to changes found in WBC counts, splenocyte counts were also altered significantly in frogs inhabiting polluted wetlands (Zhelev et al, 2014c). Sublethal doses of Cd were reported to increase agglutination titers of hemoagglutination assay in adult Rana pipiens and larvae of Rana catesbeiana (Zettergren et al, 1988(Zettergren et al, , 2001.…”

Section: Introductionmentioning

confidence: 82%

Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Priyadarshani

Madhushani

Jayawardena

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Introductionmentioning

confidence: 82%

Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Priyadarshani

Madhushani

Jayawardena

et al. 2015

Ecotoxicology and Environmental Safety

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“…This becomes important since it is known that local people hunt this species in the wild and consume them, which could, considering hygiene and health, put human health at risk. In this sense, biomarkers are essential for assessing environmental health and the state of the population (NRC 1987; Zhelev et al., 2016; Salinas et al., 2017). In particular, studies with amphibians have begun to incorporate leukocyte count as an endpoint because of their value in assessing the health of these animals (Cabagna et al., 2005; Barni et al., 2007; Davis et al., 2010; Salinas et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Effect of parasitism of Lernaea cyprinacea on tadpoles of the invasive species Lithobates catesbeianus

Salinas

Babini

Grenat

et al. 2019

Heliyon

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The introduction of invasive species is one of the greatest threats currently faced by natural ecosystems, causing ecological imbalances between native populations and transmission of a variety of diseases. We reported the interaction between two exotic species given by the parasitic infestation of the copepod Lernaea cyprinacea in the early stages of the development of the American bullfrog Lithobates catesbeianus in the central area of Argentina. In this paper we analysed the leukocyte profile of parasitized and non-parasitized tadpoles of L. catesbeianus with L. cyprinacea and their body condition (BC) as biomarkers of the health status of organisms. A total of 27 tadpoles of L. catesbeianus were analysed (12 non-parasitized and 15 parasitized). The lower BC recorded in parasitized organisms show a lower health status in these tadpoles, which could be affecting the metamorphosis and therefore impact at the population level. Leukocyte response of L. catesbeianus tadpoles to the parasitism of L. cyprinacea was found. Mature and immature lymphocyte frequencies and hematocrit were higher in parasitized compared to non-parasitized tadpoles, which is a typical response to the presence of parasites. However, eosinophils and monocytes were recorded at high frequencies in not parasitized tadpoles, which could be due to the important role played by these leucocytes in the metamorphosis of frogs. The results of this study constitute a first antecedent on leukocyte profile in aquatic stages of anurans during an ectoparasitosis and its possible implications for environmental health. The parasitism of L. cyprinacea influences the biology of the American bullfrog at both the individual and population levels. Parasitized individuals are not killed directly by the parasite, but they can create conditions for secondary infections, growth retardation, behavioral changes and, ultimately, reduce populations.

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“…In another study, it was expressed that the ES and NS values of frogs living in the mine waste wetland decrease (Pollo et al, 2016). Studies also show that NR obtained through ES and NS values in frogs are being affected and changed by anthropogenic pollution (Zhelev et al, 2016(Zhelev et al, , 2017b. According to Pollo et al (2019), the frequencies of EE, ME, PE, and IE increased in frogs (Odontophrynus, Anura: Odontophrynidae) living in agricultural areas.…”

Section: Discussionmentioning

confidence: 97%

The morphometric and erythrometric analyses of Pelophylax ridibundus living in anthropogenic pollution resources

Dönmez¹,

Şi̇şman²

2021

Turk J Zool

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The health status of Pelophylax ridibundus species living in anthropogenic pollution sources in Erzurum (Turkey) were investigated. Firstly, heavy metal concentrations of water and sediment samples taken from where the frogs live and in the frog liver and were determined by instrumental analysis. Secondly, morphometric (fluctuating asymmetry-FA, hepatosomatic index-HSI) and erythrometric analyses were performed to reveal the health status of the frogs in the areas. Our study provides the first morphometric datas on the frog in Turkey. Although the metal concentrations in the surface water were high, it was determined that they did not exceed the limit values. However, Cr, Ni, Zn, and As metal concentrations in the sediment were determined to be above the acceptable level. It was also found that some metals accumulated in the livers of the frog due to heavy metal pollution. On the other hand, the FA, HSI values and the erythrocytic nuclear abnormality frequency increased in the frogs living in polluted stations. Moreover, erythrocyte size decreased and nucleated erythrocyte, mitotic erythrocyte, pycnotic erythrocyte, and immature erythrocyte frequencies increased. It was found that there was a correlation between the presence of pollution and the health status 2 of the frogs, and the frog populations were affected negatively by anthropogenic pollution.

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Seasonal changes of basic erythrocyte-metric parameters in Pelophylaxridibundus (Amphibia: Ranidae) from anthropogenically polluted biotopes in Southern Bulgaria and their role as bioindicators

Cited by 13 publications

References 19 publications

Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Heavy metal mediated immunomodulation of the Indian green frog, Euphlyctis hexadactylus (Anura:Ranidae) in urban wetlands

Effect of parasitism of Lernaea cyprinacea on tadpoles of the invasive species Lithobates catesbeianus

The morphometric and erythrometric analyses of Pelophylax ridibundus living in anthropogenic pollution resources

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