“…Our results also show that plankton and biofilm communities are highly dissimilar within sites, within ponds and among ponds, confirming and extending previous studies reporting strong differentiation among bacterial plankton and biofilm communities in freshwater lakes (Hiraki et al ., ; Tsuchiya et al ., ; Wang et al ., ), marshes (Buesing et al ., ) and streams (Beier et al ., ; Besemer et al ., ). Already at the class level, bacterioplankton and biofilm communities were differentiated, with bacterioplankton having higher levels of Betaproteobacteria and Actinobacteria compared with biofilm communities, and biofilm communities having higher levels of Alphaproteobacteria, Gammaproteobacteria and unidentified Bacteria, in line with literature data (Zwart et al ., ; Eiler and Bertilsson, ; Hempel et al ., ; Buesing et al ., ; Newton et al ., ; Besemer et al ., ; Zhang et al ., ).…”