Seasonal and spatial patterns of microbial diversity along a trophic gradient in the interconnected lakes of the Osterseen Lake District, Bavaria

Zwirglmaier, Katrin; Keiz, Katharina; Engel, Marion; Geist, Juergen; Raeder, Uta

doi:10.3389/fmicb.2015.01168

Cited by 55 publications

(39 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thanks to the development of next generation sequencing (NGS), we can present the biodiversity of the picoeukaryotes at a finer scale. It has been reported that investigations of microbial community on the basis of changes in highly abundant taxa or only at higher taxonomic levels may mask distinct patterns in microbial distribution (Zwirglmaier et al ., ). At the phylum or class levels, little variation of the picoeukaryotes community was unveiled in our samples (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…The quality filtering step eliminated about 17% of all valid merged sequences. Lastly, the remaining reads were pooled together, dereplicated and clustered to define operational taxonomic units (OTUs) using cluster_otus algorithm at a 97% similarity threshold (Zwirglmaier et al, 2015). After removing the singletons (OTUs with only one read), 1580 OTUs were defined.…”

Section: Sequence Processing and Analysismentioning

confidence: 99%

See 1 more Smart Citation

Temporal and spatial variations in the composition of freshwater photosynthetic picoeukaryotes revealed by MiSeq sequencing from flow cytometry sorted samples

Bronner

Lepère

et al. 2017

Environmental Microbiology

View full text Add to dashboard Cite

The diversity and composition of photosynthetic picoeukaryotes (PPEs) in two large shallow lakes in China (Lake Taihu and Lake Chaohu) were investigated from flow cytometry sorted samples using Miseq high-throughput sequencing. We collected 65 samples covering different regions of the two lakes over four seasons to unveil spatial and temporal patterns of PPEs community composition. The use of flow cytometry sorting largely improved the efficiency of detecting PPEs sequences and over 70% of the retrieved reads belonged to PPEs. Chlorophyta and Bacillariophyta dominated PPEs in most of the samples. A distinct but complex seasonality of PPEs composition emerged at the OTUs level. NGS-based Miseq sequencing facilitates an in-depth view of numerous rare OTUs. Nearly 80% of the PPEs OTUs were rare and lots of them were detected only in one season, whereas most of the abundant OTUs were frequently detected in all seasons but only changed in relative abundances. Besides, a close relative of the marine PPEs species Ostreococcus sp. (OTU_1144, 99% identity) was discovered in freshwater systems for the first time and was abundant especially in winter. The diversity and community composition of PPEs were more dependent on season rather than sampling sites. Temperature, phycocyanin and NO N concentrations in Lake Taihu explained the PPE composition variations, whereas in Lake Chaohu TN/TP ratios, temperature, pH and nephelometric turbidity units (NTU) seemed to be the most important factors. In addition, a great number of OTUs belong to nonpigmented picoeukaryotes, especially Chytridiomycota, Perkinsozoa, Ciliophora and Cercozoa, which are known to include algae parasites as well as predators. The results of mantel test also showed that the community of photosynthetic and nonpigmented picoeukaryotes were significantly correlated in both lakes.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Sequence Processing and Analysismentioning

confidence: 99%

Temporal and spatial variations in the composition of freshwater photosynthetic picoeukaryotes revealed by MiSeq sequencing from flow cytometry sorted samples

Bronner

Lepère

et al. 2017

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…UPGMA clustering of bacterial communities indicated an apparent spatial variation in bacterial communities in the lake and the streams. In general, physicochemical parameters, nutrients, and nutrient ratios could drive bacterial communities in freshwater ecosystems [34,[47][48][49][50][51]. Our results demonstrated that N-factor (NO 3 − ) (7%) and the interaction of multiple factors, including physicochemical-factor, N-factor, P-factor, and C-factor, drove the bacterial community structure on stream biofilms (Figure 7f).…”

Section: Discussionmentioning

confidence: 66%

Impact of Nutrient and Stoichiometry Gradients on Microbial Assemblages in Erhai Lake and Its Input Streams

Liu

Elser

et al. 2019

Water

View full text Add to dashboard Cite

Networks of lakes and streams are linked by downslope flows of material and energy within catchments. Understanding how bacterial assemblages are associated with nutrients and stoichiometric gradients in lakes and streams is essential for understanding biogeochemical cycling in freshwater ecosystems. In this study, we conducted field sampling of bacterial communities from lake water and stream biofilms in Erhai Lake watershed. We determined bacterial communities using high-throughput 16S rRNA gene sequencing and explored the relationship between bacterial composition and environmental factors using networking analysis, canonical correspondence analysis (CCA), and variation partitioning analysis (VPA). Physicochemical parameters, nutrients, and nutrient ratios gradients between the lake and the streams were strongly associated with the differences in community composition and the dominant taxa. Cyanobacteria dominated in Erhai Lake, while Proteobacteria dominated in streams. The stream bacterial network was more stable with multiple stressors, including physicochemical-factors and nutrient-factors, while the lake bacterial network was more fragile and susceptible to human activities with dominant nutrients (phosphorus). Negative correlations between bacterial communities and soluble reactive phosphorus (SRP) as well as positive correlations between bacterial communities and dissolved organic carbon (DOC) in the network indicated these factors had strong effect on bacterial succession. Erhai Lake is in a eutrophic state, and high relative abundances of Synechococcus (40.62%) and Microcystis (16.2%) were noted during the course of our study. CCA indicated that nutrients (phosphorus) were key parameters driving Cyanobacteria-dominated community structure. By classifying the environmental factors into five categories, VPA analyses identified that P-factor (total phosphorus (TP) and SRP) as well as the synergistic effect of C-factor (DOC), N-factor (NO3−), and P-factor (TP and SRP) played a central role in structuring the bacterial communities in Erhai Lake. Heterogeneous physicochemical conditions explained the variations in bacterial assemblages in streams. This study provides a picture of stream–lake linkages from the perspective of bacterial community structure as well as key factors driving bacterial assemblages within lakes and streams at the whole watershed scale. We further argue that better management of phosphorus on the watershed scale is needed for ameliorating eutrophication of Erhai Lake.

show abstract

“…These results may suggest that MC-degrading bacterioplankton community diversity may increase with trophic status (from oligo to fully eutrophic) of freshwater lakes. However, as indicated by [67][68][69], the relationship between trophic status and community diversity of bacterioplankton is not so clear-cut, as other studies have reported contradictory trends [68,69]. Furthermore, it is still unclear how diversity may influence MC biodegradation kinetics in the environment as the temporal stability of these bacterioplankton communities is relatively unknown (i.e., do they achieve a relative steady state?).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

Manheim

Cheung

Jiang

2018

Water

View full text Add to dashboard Cite

Microcystin (MC), a hepatotoxin that is associated with cyanobacterial blooms in freshwater lakes, threatens the quality of drinking water resources. Biodegradation of MC using biofiltration is emerging as a cost-effective solution for drinking water treatment. This study reports isolation of five MC-degrading microbial consortia and investigation of their community structure and kinetics in the presence or absence of a readily-bioavailable organic carbon source. The results indicated that the presence of a bioavailable organic carbon source caused: (1) the proliferation of community members previously unobserved in each consortium cultured without ethanol; (2) a shift in abundance of representative taxa; (3) a fluctuation in genera affiliated with MC-biodegradation; and, (4) a unique response in simulated diversity among consortia. These changes to each microbial consortium were paralleled by a significant decline in MC degradation kinetics. Overall, this study highlights the importance of integrating environmental conditions into the design and operation of biofiltration systems for MC biodegradation.Previous studies have demonstrated the complete biodegradation of MC-LR within laboratory scale biofiltration systems following a preliminary lag phase of removal [14][15][16][17]. However, the toxin removal efficiency and extent of this lag phase can vary significantly with bacterial composition, nutrient concentration, and other environmental parameters that are associated with the source water. Efforts to evolve biofiltration from a passive process into a more standardized, controlled, and perhaps "engineered", biological treatment process for targeted removal of pollutants will require a better understanding of the physiology and genetics of MC-degrading bacteria [12][13][14][18][19][20].Significant efforts have been made to isolate and characterize the specific MC-degrading bacterial populations during algal bloom events in the source water and sediments, and from full scale biological treatment units in drinking water treatment facilities [7][8][9]17,[21][22][23][24]. However, these studies have not fully explored how these isolates function in mixed bacterial communities, nor the influences of mutualistic or antagonistic interactions on biodegradation kinetics. Several previous studies, however, have considered the effects of environmental stimuli (i.e., varying organic carbon concentrations) on MC-degrading bacterial consortia under aerobic conditions [7,8,23,[25][26][27][28][29]. Research showed that addition of organic carbon sources (i.e., glucose, acetate, or uncharacterized dissolved organic carbon (DOC)) significantly inhibits MC degradation kinetics of bacterial consortia [7,23,25,29]. Catabolite repression is postulated as a mechanism underlying this inhibition, where MC-degrading populations may prefer more energetically-favorable (easily metabolized) over more energetically-intensive substrates, such as MC [30]. Other (fewer) studies have demonstrated that the addition of an alternative organic ca...

show abstract

Seasonal and spatial patterns of microbial diversity along a trophic gradient in the interconnected lakes of the Osterseen Lake District, Bavaria

Cited by 55 publications

References 49 publications

Temporal and spatial variations in the composition of freshwater photosynthetic picoeukaryotes revealed by MiSeq sequencing from flow cytometry sorted samples

Temporal and spatial variations in the composition of freshwater photosynthetic picoeukaryotes revealed by MiSeq sequencing from flow cytometry sorted samples

Impact of Nutrient and Stoichiometry Gradients on Microbial Assemblages in Erhai Lake and Its Input Streams

The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

Contact Info

Product

Resources

About