Seasonal abundance and potential of Japanese encephalitis virus infection in mosquitoes at the nesting colony of ardeid birds, Thailand

Changbunjong, Tanasak; Weluwanarak, Thekhawet; Taowan, N. A.; Suksai, Parut; Chamsai, Tatiyanuch; Sedwisai, Poonyapat

doi:10.1016/s2221-1691(13)60051-9

Cited by 20 publications

(13 citation statements)

References 13 publications

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“…Mosquitoes used in this study were derived from wild‐caught larvae of O. detritus sourced locally and Culex quinquefasciatus Say (Recife strain), a colonized mosquito from Brazil maintained at the Liverpool School of Tropical Medicine (LTSM). Culex quinquefasciatus was used for validation because JEV has been isolated from this mosquito previously (Weng et al , ; Halstead & Tsai, ; Nitatpattana et al , ; Changbunjong et al , ) and found to be competent for JEV in infection studies (Mourya et al , ; van den Hurk et al , ; Liu et al , ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a temperate climate mosquito, Ochlerotatus detritus (=Aedes detritus), as a potential vector of Japanese encephalitis virus

Mackenzie-Impoinvil

Impoinvil

Galbraith

et al. 2014

Medical Vet Entomology

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The U.K. has not yet experienced a confirmed outbreak of mosquito-borne virus transmission to people or livestock despite numerous autochthonous epizootic and human outbreaks of mosquito-borne diseases on the European mainland. Indeed, whether or not British mosquitoes are competent to transmit arboviruses has not been established. Therefore, the competence of a local (temperate) British mosquito species, Ochlerotatus detritus (=Aedes detritus) (Diptera: Culicidae) for transmission of a member of the genus Flavivirus, Japanese encephalitis virus (JEV) as a model for mosquito-borne virus transmission was assessed. The JEV competence in a laboratory strain of Culex quinquefasciatus (Diptera: Culicidae), a previously incriminated JEV vector, was also evaluated as a positive control. Ochlerotatus detritus adults were reared from field-collected juvenile stages. In oral infection bioassays, adult females developed disseminated infections and were able to transmit virus as determined by the isolation of virus in saliva secretions. When pooled at 7-21 days post-infection, 13% and 25% of O. detritus were able to transmit JEV when held at 23 °C and 28 °C, respectively. Similar results were obtained for C. quinquefasciatus. To our knowledge, this study is the first to demonstrate that a British mosquito species, O. detritus, is a potential vector of an exotic flavivirus.

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Section: Methodsmentioning

confidence: 99%

Evaluation of a temperate climate mosquito, Ochlerotatus detritus (=Aedes detritus), as a potential vector of Japanese encephalitis virus

Mackenzie-Impoinvil

Impoinvil

Galbraith

et al. 2014

Medical Vet Entomology

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“…subalbatus transmitting pathogens to humans in Thailand, it has been shown as an efficient vector of the dog heartworm, Dirofilaria immitis [ 52 ]. Culex quinquefasciatus is presently regarded as an important vector of filariasis and Japanese encephalitis in the tropical and subtropical regions [ 53 – 55 ]. However, the risk from mosquito-borne diseases has not been reported in the area of the present study.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Angelica sinensis (Oliv.) Diels as a repellent for personal protection against mosquitoes under laboratory and field conditions in northern Thailand

et al. 2016

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BackgroundAngelica sinensis (Oliv.) hexane extract (AHE) has been reported as a proven and impressive repellent against laboratory-reared female Aedes aegypti mosquitoes. With the aim of promoting products of plant origin as a viable alternative to conventional synthetic substances, this study was designed to transform AHE-based repellents for exploitable commercial production by enhancing their efficacy and assessing their physical and biological stability as well as repellency against mosquitoes under laboratory and field conditions.MethodsThe chemical profile of AHE was analyzed by qualitative gas chromatography-mass spectrometry (GC-MS) technique. AHE was supplemented with vanillin, as a fixative, and then investigated for repellency and comparison to the standard synthetic repellent, DEET, under both laboratory and field conditions. Determination of physical and biological stability as a repellent was carried out after keeping AHE samples under varying temperatures and for different storage times.ResultsGC-MS analysis revealed that AHE contained at least 21 phytochemical compounds, constituting 95.74 % of the total content, with the major constituent of 3-N-butylphthalide (66.67 %). Ethanolic formulations of AHE and DEET showed improvement of repellency in a dose-dependent manner when vanillin was added in laboratory assessment. While 5–25 % AHE alone provided median complete-protection times of 2.0–6.5 h against Ae. aegypti, these times were increased to 4.0–8.5 h with a combination of AHE and 5 % vanillin (AHEv). Protection times against Ae. aegypti were extended from 2.25 to 7.25 h to 4.25–8.25 h when 5–25 % DEET was combined with 5 % vanillin (DEETv). In determining stability, all stored AHE samples exhibited similar characteristics such as liquid phases with aromatic odor comparable to those of fresh preparations. Furthermore, repellent activity of stored AHE samples lasted for at least six months, with varied efficacy (4.5–10.0 h) against Ae. aegypti. Field trials revealed strong repellency from both 25 % AHEv and 25 % DEETv, with complete protection (100 %) against a wide range of local mosquito populations. A total of 5,718 adult female mosquitoes, with the most predominant being Culex quinquefasciatus (41.47 %), Armigeres subalbatus (41.13 %), and Culex vishnui (10.53 %), was collected during field applications. No local skin reaction or other allergic responses was observed during both laboratory and field study periods.ConclusionsAngelica sinensis proved to have not only impressive repellency against both laboratory Ae. aegypti and a wide range of natural mosquito populations, but also relative stability in physical and biological performance.

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“…Culex tritaeniorhynchus has been demonstrated to be the primary vector for JEV in China and most other Asia countries 3 7 15 16 . In this study, Culex tritaeniorhynchus constitutes a dramatic proportion (86.2%) of total mosquitoes collected, and it is hypothesized that the extended rainy season from August to September in 2013 is believed to be responsible for the large amount of Culex tritaeniorhynchus populations, as is similar with the situation in ROK in 2010 3 .…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes during an Outbreak in China, 2013

Tao

Liu

Wang

et al. 2014

Sci Rep

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Japanese encephalitis virus (JEV) can cause serious encephalitis and Culex mosquitoes are the primary vector. In 2013, a JE outbreak occurred in Shandong Province, China with 407 confirmed cases, including 11 deaths. An investigation on JEV in mosquitoes during the outbreak was conducted. A total of 14,719 mosquitoes were collected at 3 sites. For the 12,695 Culex tritaeniorhynchus mosquitoes, 88/201 pooled samples were positive by RT-PCR for the presence of the pre-membrane or envelope protein coding genes. The maximum likelihood estimates of JEV positive individuals per 1,000 vectors were 12.0, 7.2, and 6.0 in the 3 sites respectively with an overall estimate of 9.1. Phylogenetic analysis on these pre-membrane (n = 72) and envelope (n = 26) sequences with those of reference strains revealed they belonged to genotype I. This study describes the molecular epidemiology of JEV and suggests the high infection rate in mosquitoes is an important factor for the outbreak.

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Seasonal abundance and potential of Japanese encephalitis virus infection in mosquitoes at the nesting colony of ardeid birds, Thailand

Cited by 20 publications

References 13 publications

Evaluation of a temperate climate mosquito, Ochlerotatus detritus (=Aedes detritus), as a potential vector of Japanese encephalitis virus

Evaluation of a temperate climate mosquito, Ochlerotatus detritus (=Aedes detritus), as a potential vector of Japanese encephalitis virus

Assessment of Angelica sinensis (Oliv.) Diels as a repellent for personal protection against mosquitoes under laboratory and field conditions in northern Thailand

Molecular Epidemiology of Japanese Encephalitis Virus in Mosquitoes during an Outbreak in China, 2013

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