Abstract:Phage Display is a powerful tool for identification of protein ligands against a myriad of targets. In our study, we selected a small protein member from the Kunitz family to serve as a scaffold in the MIII 13 phage coat protein. The protein, originally a scorpion toxin that inhibits a potassium‐dependent voltage channel, has 33 residues composed of two beta sheets and an alpha helix stabilized by disulfide bonds. After cloning the toxin gene into an M13 phagemid, we used Kunkel's mutagenesis, an additional ur… Show more
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