Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

Lesiak, Marta; Auguściak‐Duma, Aleksandra; Stępień, Karolina; Fus-Kujawa, Agnieszka; Botor, Malwina; Sieroń, Aleksander

doi:10.1007/s13353-021-00641-4

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…In addition, noncoding RNAs such as microRNA are biological molecules whose expression can be modified through targeted mechanisms and present opportunities to identify new treatment options for patients with aortic disease. 1-7 In addition, developing image-based cardiac and aortic markers derived from large-scale imaging studies with automated machine learning–based analysis might provide a wealth of information for guiding the optimal care of these patients.…”

Section: Evidence Gaps and Future Directionsmentioning

confidence: 99%

2022 ACC/AHA Guideline for the Diagnosis and Management of Aortic Disease: A Report of the American Heart Association/American College of Cardiology Joint Committee on Clinical Practice Guidelines

Isselbacher¹,

Preventza²,

Black³

et al. 2022

Circulation

639

410

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Aim: The “2022 ACC/AHA Guideline for the Diagnosis and Management of Aortic Disease” provides recommendations to guide clinicians in the diagnosis, genetic evaluation and family screening, medical therapy, endovascular and surgical treatment, and long-term surveillance of patients with aortic disease across its multiple clinical presentation subsets (ie, asymptomatic, stable symptomatic, and acute aortic syndromes). Methods: A comprehensive literature search was conducted from January 2021 to April 2021, encompassing studies, reviews, and other evidence conducted on human subjects that were published in English from PubMed, EMBASE, the Cochrane Library, CINHL Complete, and other selected databases relevant to this guideline. Additional relevant studies, published through June 2022 during the guideline writing process, were also considered by the writing committee, where appropriate. Structure: Recommendations from previously published AHA/ACC guidelines on thoracic aortic disease, peripheral artery disease, and bicuspid aortic valve disease have been updated with new evidence to guide clinicians. In addition, new recommendations addressing comprehensive care for patients with aortic disease have been developed. There is added emphasis on the role of shared decision making, especially in the management of patients with aortic disease both before and during pregnancy. The is also an increased emphasis on the importance of institutional interventional volume and multidisciplinary aortic team expertise in the care of patients with aortic disease.

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Section: Evidence Gaps and Future Directionsmentioning

confidence: 99%

2022 ACC/AHA Guideline for the Diagnosis and Management of Aortic Disease: A Report of the American Heart Association/American College of Cardiology Joint Committee on Clinical Practice Guidelines

Isselbacher¹,

Preventza²,

Black³

et al. 2022

Circulation

639

410

View full text Add to dashboard Cite

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“…Infiltration of macrophages is associated with increased activity of matrix metalloproteinases 2 and 9, which lead to degradation of the ECM and weaken the aneurysmal wall [26]. In our recent studies, we have shown elevated expression of genes associated with the inflammation process in AAA, e.g., CD68, IL1R2 [10,27]. Flow cytometry of cells from the EL of AAA showed that these cells are positive for CD90 and anti-fibroblast marker, but their numbers were much lower compared with controls.…”

Section: Cd68mentioning

confidence: 95%

“…The phenotypes of the cells were determined by fluorescence-assisted flow cytometry using the Facs Aria I instrument (Becton Dickinson, Franklin Lakes, NJ, USA) as described in detail elsewhere [10]. For…”

Section: Fluorescence-assisted Cell Flow Cytometry Analysismentioning

confidence: 99%

Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro

et al. 2022

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Introduction:This study aimed to standardize cell culture methods for major cell types isolated from three layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare them with cell types in layers of control, unchanged segments. Material and methods: We divided AAAs into three segments along the AAA and control segments flanking the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, and microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types (endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment. Results: The culture of cells from the three AAA segments was successfully established in 21% of patients. In all of the layers, only a small proportion of cells showed layer-specific markers of cell types. The majority of cells from every layer were positive for CD90, which is considereda specific marker of fibroblasts in the aorta. Conclusions: We describe a methodology for isolation of cells, their culture conditions, and phenotypic characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the normal abdominal aortic wall.

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“…AAA is a complex disease, and each case is slightly different [ 82 ]. This phenomenon is due to various factors such as environmental, genetic, and molecular.…”

Section: Conclusion/perspectivesmentioning

confidence: 99%

“…Extracellular matrix degradation and inflammation are considered to be the most important molecular processes contributing to AAA formation. The initiating molecular processes cause matrix degradation, cell apoptosis, and aging, as well as infiltration of inflammatory cells such as lymphocytes, mast cells, and neutrophils ( Figure 5 ) [ 82 , 83 , 84 ].…”

Section: Conclusion/perspectivesmentioning

confidence: 99%

Role of Extracellular Matrix and Inflammation in Abdominal Aortic Aneurysm

Stępień

Bajdak-Rusinek²,

Fus-Kujawa³

et al. 2022

IJMS

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Abdominal aortic aneurysm (AAA) is one of the most dangerous cardiovascular diseases, occurring mainly in men over the age of 55 years. As it is asymptomatic, patients are diagnosed very late, usually when they suffer pain in the abdominal cavity. The late detection of AAA contributes to the high mortality rate. Many environmental, genetic, and molecular factors contribute to the development and subsequent rupture of AAA. Inflammation, apoptosis of smooth muscle cells, and degradation of the extracellular matrix in the AAA wall are believed to be the major molecular processes underlying AAA formation. Until now, no pharmacological treatment has been implemented to prevent the formation of AAA or to cure the disease. Therefore, it is important that patients are diagnosed at a very early stage of the disease. Biomarkers contribute to the assessment of the concentration level, which will help to determine the level and rate of AAA development. The potential biomarkers today include homocysteine, cathepsins, osteopontin, and osteoprotegerin. In this review, we describe the major aspects of molecular processes that take place in the aortic wall during AAA formation. In addition, biomarkers, the monitoring of which will contribute to the prompt diagnosis of AAA patients over the age of 55 years, are described.

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Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

Cited by 7 publications

References 40 publications

2022 ACC/AHA Guideline for the Diagnosis and Management of Aortic Disease: A Report of the American Heart Association/American College of Cardiology Joint Committee on Clinical Practice Guidelines

2022 ACC/AHA Guideline for the Diagnosis and Management of Aortic Disease: A Report of the American Heart Association/American College of Cardiology Joint Committee on Clinical Practice Guidelines

Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro

Role of Extracellular Matrix and Inflammation in Abdominal Aortic Aneurysm

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