Searching for differentially expressed proteins in spinal cord injury based on the proteomics analysis

Ding, Hengfei; Yu, Jia; Chang, Wenju; Liu, Fendou; He, Zhenxing

doi:10.1016/j.lfs.2019.117235

Cited by 12 publications

(12 citation statements)

References 68 publications

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“…A previous study by Squair et al that involved integrated systems analysis showed that the module of coexpressed genes enriched for markers of microglia, inflammatory response, and response to wounding was correlated with the severity of SCI 4 . Furthermore, Ding et al used the isobaric tags for relative and absolute quantization analysis and demonstrated that the peroxisome proliferator‐activated receptors and vascular endothelial growth factor were associated with the repair of SCI 5 . However, few studies have examined the time‐related proteomic changes after SCI and revealed the potential networks involved in the pathological progression of SCI.…”

Section: Introductionmentioning

confidence: 99%

“…4 Furthermore, Ding et al used the isobaric tags for relative and absolute quantization analysis and demonstrated that the peroxisome proliferator-activated receptors and vascular endothelial growth factor were associated with the repair of SCI. 5 However, few studies have examined the time-related proteomic changes after SCI and revealed the potential networks involved in the pathological progression of SCI.…”

Section: Introductionmentioning

confidence: 99%

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Proteomics and bioinformatics reveal insights into neuroinflammation in the acute to subacute phases in rat models of spinal cord contusion injury

et al. 2021

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Neuroinflammation is recognized as a hallmark of spinal cord injury (SCI). Although neuroinflammation is an important pathogenic factor that leads to secondary injuries after SCI, neuroprotective anti‐inflammatory treatments remain ineffective in the management of SCI. Moreover, the molecular signatures involved in the pathophysiological changes that occur during the course of SCI remain ambiguous. The current study investigated the proteins and pathways involved in C5 spinal cord hemi‐contusion injury using a rat model by means of 4‐D label‐free proteomic analysis. Furthermore, two Gene Expression Omnibus (GEO) transcriptomic datasets, Western blot assays, and immunofluorescent staining were used to validate the expression levels and localization of dysregulated proteins. The present study observed that the rat models of SCI were associated with the enrichment of proteins related to the complement and coagulation cascades, cholesterol metabolism, and lysosome pathway throughout the acute and subacute phases of injury. Intriguingly, the current study also observed that 75 genes were significantly altered in both the GEO datasets, including ANXA1, C1QC, CTSZ, GM2A, GPNMB, and PYCARD. Further temporal clustering analysis revealed that the continuously upregulated protein cluster was associated with immune response, lipid regulation, lysosome pathway, and myeloid cells. Additionally, five proteins were further validated by means of Western blot assays and the immunofluorescent staining showed that these proteins coexisted with the F4/80+ reactive microglia and infiltrating macrophages. In conclusion, the proteomic data pertaining to the current study indicate the notable proteins and pathways that may be novel therapeutic targets for the treatment of SCI.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomics and bioinformatics reveal insights into neuroinflammation in the acute to subacute phases in rat models of spinal cord contusion injury

et al. 2021

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“…As a vital member of the peroxisome proliferators-activated receptor (PPAR) family, PPARα serves as an essential ligand-activated transcription factor that represses inflammatory responses by inhibiting pro-inflammatory cytokines’ products, adhesion molecules and extracellular matrix proteins [ 10 , 11 ]. The anti-inflammatory mechanism of PPARα is primarily associated with the activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) and the inhibition of NF-κB.…”

Section: Introductionmentioning

confidence: 99%

PPARα agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway

Zhang

Xiang

et al. 2021

Aging

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Objective: To observe the inhibitory effects of the peroxisome proliferator-activated receptor alpha (PPARα) agonist palmitoylethanolamide (PEA) on inflammatory responses and oxidative stress injury in rats with spinal cord injury (SCI). Methods: The SCI rat model was established using modified Allen's method and the changes in rats’ joint motion were observed by Basso, Beattie and Bresnahan locomotor rating scale (BBB scale) at 1, 3 and 7 days after modeling, HE Staining and Nissl Staining has been carried out to evaluate the pathological lesion of spinal cords in rats. Besides, Immunohistochemical (IHC) was performed to detect the reactive oxygen species (ROS), expression levels of acrylamide (ACR) and manganese superoxide dismutase (MnSOD) in rat spinal cords, and Western Blotting was applied to measure protein expression levels of nuclear factor-kappa B (NF-κB), B cell lymphoma-2 (Bcl-2), BCL-2 associated X (BAX), phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), phosphorylated (p)-Akt, HO-1, Nrf2, trithorax-1 (TRX-1), Raf-1, MEK, ERK, p-MEK and p-ERK. Results: The PPARα agonist PEA could alleviate SCI in rats, inhibit inflammatory responses, mitigate oxidative stress injury, reduce the apoptotic rate and promote SCI rats motor function recovery. In addition, the PPARα agonist PEA was able to activate the phosphorylation of MEK and ERK, stimulate Nrf-2 translocation into the nucleus and up-regulate the expressions of HO-1 and TRX-1. Conclusion: PPARα agonist PEA can relieve SCI in rats by inhibiting inflammatory responses and oxidative stress, which may involve a mechanism associated with the activation of Nrf2/HO-1 via the Raf-1/MEK/ERK pathway.

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“…This suggests that down-regulation of S100A6 is involved in early posttraumatic events that lead to secondary cognitive disorders while the elevation of S100A6 level in time is implicated in neuronal regeneration and repair. This possibility is supported by proteomic studies that showed increased S100A6 level in the spinal cord after injury [51]. Interestingly, down-regulation of S100A6 expression has been observed in the brainstem, hippocampus, and hypothalamus of mice subjected to the unpredictable stress paradigm [18].…”

Section: S100a6 and Other Neurodegenerative Diseasesmentioning

confidence: 83%

S100A6 and Its Brain Ligands in Neurodegenerative Disorders

Filipek

2020

IJMS

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The S100A6 protein is present in different mammalian cells and tissues including the brain. It binds Ca2+ and Zn2+ and interacts with many target proteins/ligands. The best characterized ligands of S100A6, expressed at high level in the brain, include CacyBP/SIP and Sgt1. Research concerning the functional role of S100A6 and these two ligands indicates that they are involved in various signaling pathways that regulate cell proliferation, differentiation, cytoskeletal organization, and others. In this review, we focused on the expression/localization of these proteins in the brain and on their possible role in neurodegenerative diseases. Published results demonstrate that S100A6, CacyBP/SIP, and Sgt1 are expressed in various brain structures and in the spinal cord and can be found in different cell types including neurons and astrocytes. When it comes to their possible involvement in nervous system pathology, it is evident that their expression/level and/or subcellular localization is changed when compared to normal conditions. Among diseases in which such changes have been observed are Alzheimer’s disease (AD), amyotrophic lateral sclerosis (ALS), epileptogenesis, Parkinson’s disease (PD), Huntington’s disease (HD), and others.

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Searching for differentially expressed proteins in spinal cord injury based on the proteomics analysis

Cited by 12 publications

References 68 publications

Proteomics and bioinformatics reveal insights into neuroinflammation in the acute to subacute phases in rat models of spinal cord contusion injury

Proteomics and bioinformatics reveal insights into neuroinflammation in the acute to subacute phases in rat models of spinal cord contusion injury

PPARα agonist relieves spinal cord injury in rats by activating Nrf2/HO-1 via the Raf-1/MEK/ERK pathway

S100A6 and Its Brain Ligands in Neurodegenerative Disorders

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