Search for the genes involved in oocyte maturation and early embryo development in the hen

Elis, Sébastien; Batellier, Florence; Couty, Isabelle; Balzergue, Sandrine; Martin‐Magniette, Marie‐Laure; Monget, Philippe; Blesbois, Élisabeth; Govoroun, Marina

doi:10.1186/1471-2164-9-110

Cited by 48 publications

(30 citation statements)

References 66 publications

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“…Dazl, however, is expressed in both male and female germ cell. Its homolog has been identified in model organisms including human (Nishi et al, '99;Brekhman et al, 2000), mouse (Ruggiu et al,'97), chicken (Yamamoto et al, 2007;Elis et al, 2008), Xenopus (Houston et al,'98), axolotl (Johnson et al, 2001), newt (Tamori et al, 2004), zebrafish (Maegawa et al,'99) and medaka (Xu et al, 2007). Loss-of-function experiments demonstrated that the Dazl gene functions in both meiosis and normal germ cell development process in several species (Ruggiu et al, '97;Houston and King, 2000b).…”

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confidence: 99%

Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp

et al. 2009

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To investigate germline development and germ cell specification, we identified a Dazl homolog (CagDazl) from gynogenetic gibel carp (Carassius auratus gibelio). Its cDNA sequence and BAC clone sequence analyses revealed the genomic organization conservation and conserved synteny of the Dazl family members and their neighborhood genes among vertebrates, especially in fish. Moreover, a polyclonal antibody specific to CagDazl was produced and used to examine its expression and distribution throughout germline development at protein level. Firstly, ovary-specific expression pattern of CagDazl was confirmed in adult tissues by RT-PCR and Western blot. In addition, in situ hybridization and immunofluorescence localization demonstrated its specific expression in germ cells, and both its transcript and protein were localized to germ plasm. Then, co-localization of CagDazl and mitochondrial cloud was found, confirming that CagDazl transcript and its protein are germ plasm component and move via METRO pathway during oogenesis. Furthermore, the CagDazl is abundant and continuous throughout germline development and germ cell specification including primordial germ cell (PGC) formation, oogonium differentiation, oocyte development, and embryogenesis, and the dynamic distribution occurs at different development stages. The data suggest that maternal CagDazl might play an important role in gibel carp PGC formation. Therefore, CagDazl is a useful and specific marker for tracing germ plasm and germ cell development in the gynogenetic gibel carp. In addition, in comparison with previous studies in sexual reproduction species, the continuous and dynamic distribution of CagDazl protein in the germ plasm throughout the life cycle seems to have significant implication in sex evolution of vertebrates.

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confidence: 99%

Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp

et al. 2009

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“…Trappins have been identified as potential treatment for emphysema, because trappin 2 is able to reduce emphysematous changes by increasing granulocyte colony-stimulating factor (G CSF) (Janelle et al, 2006). TRAP6 was identified as an oocyte gene in our previous study (Elis et al, 2008). Indeed, according to microarray experiments, this gene was more highly expressed in GDR at the F1 stage than in granulosa cells at the same stage or in GDR at the ovulation stage.…”

Section: Discussionmentioning

confidence: 93%

“…Germinal disc regions (GDR) were collected as previously described (Malewska and Olszanska, 1999;Elis et al, 2008) from different preovulatory follicles (F1-F3), from recently (1-5 min) ovulated oocytes and from early embryos at 6.5, 24, and 48 hr postovulation. The GDR from preovulatory follicles consisted of the germinal disc and of the overlying layer of GCs, because GD and overlying GCs cannot be separated (Elis et al, 2008). The 6.5 and 24 hr stages of embryo development correspond to stages I and X of the Eyal-Giladi and Kochav (1976) classification, respectively.…”

Section: Collection Of Tissue Oocytes and Embryosmentioning

confidence: 99%

“…The hens exhibiting lowest and highest fertility results were chosen for sampling GDR at F1 stage for microarray hybridization. After dissection of the GDR, total RNA was extracted from the GDR of F1 stage follicles of DPFþ, DPFÀ and HG hen lines as previously described (Elis et al, 2008). Briefly, the MasterPureÔ RNA Purification Kit (Epicentre Biotechnologies, Madison, WI) was used according to the manufacturer's instructions.…”

Section: Microarray Analysesmentioning

confidence: 99%

“…Four experimental conditions were used: DPFþ hens, DPFÀ hens with lowest fertility, HG hens with highest fertility and HG hens with lowest fertility. Labeled cRNA synthesis and microarray hybridization were performed as previously described (Elis et al, 2008). The Affymetrix GeneChip Ò Chicken Genome Arrays were finally scanned with the GeneChip Ò Scanner 3000 7G piloted by GeneChip Ò Operating Software (GCOS).…”

Section: Microarray Analysesmentioning

confidence: 99%

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Identification of germinal disk region derived genes potentially involved in hen fertility

Elis¹,

Blesbois²,

Couty³

et al. 2009

Molecular Reproduction Devel

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Despite the regular decrease in fertility observed in hens, especially in "meat" lines, little is known about genes affecting fertility. We used the Affymetrix microarray to search for oocyte genes whose expression would vary in relation to fertility rate in both "laying" and "meat" line hens. We focused on oocyte genes because several of them have been found to be involved in fertility in other species. Based on microarray analysis, 54 and 84 genes were differentially expressed between germinal disc regions (GDR) of F1 maturation stage oocytes from hens exhibiting either high (100%) or low (from 22% to 80%) fertility rate from laying and meat lines respectively. Most of these differentially expressed genes were distributed between "laying" and "meat" lines indicating that mechanisms involved in the decrease in fertility rates in these two cases were independent. Real time RT-PCR performed on the same samples which were used for microarray confirmed in several cases differences in gene expression levels detected by microarray. Moreover the correlations between gene expression levels and fertility rates were evaluated for the 10 most interesting genes at different stages of follicular maturation and early embryo development on individual GDR samples from hens exhibiting different fertility rates. In total, we identified five genes whose expression levels correlated with fertility rate in accordance with findings of microarray analysis and real time RT-PCR: VWC2, CR407412, TAPA, FGL2, and TRAP6. The biological significance of these genes sheds light on potential mechanisms influencing fertility and could provide candidates for fertility markers in the hen.

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Comparison of extraembryonic expression of Eomes and Cdx2 in pregastrulation chick and mouse embryo unveils regulatory changes along evolution

Pernaute

Cañón

Crespo

et al. 2010

Developmental Dynamics

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In the mouse blastocyst, Eomes and Cdx2 are critical for establishing the trophoectoderm, the precursor of the placenta. To better understand how the trophoectoderm lineage arose in mammals during evolution, we examined the expression of their orthologues in the pregastrulation chick embryo and found that, while both genes are expressed in extraembryonic tissues, their temporal pattern of expression differs from what occurs in mouse. Moreover, we failed to detect expression of other genes specific from the mouse trophoectoderm in extraembryonic regions of the chick. Also unlike the mouse, chick Eomes is expressed in primordial germ cells. Finally, conserved noncoding elements in the Eomes genomic region are unable to drive trophoectoderm restricted expression in the mouse blastocyst, but do so in conserved sites of expression such as the forebrain. These results suggest that critical changes in the gene regulatory networks controlling extraembryonic development accompanied the appearance of the trophoectoderm in mammals. Developmental Dynamics 239:620-629,

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Search for the genes involved in oocyte maturation and early embryo development in the hen

Cited by 48 publications

References 66 publications

Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp

Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp

Identification of germinal disk region derived genes potentially involved in hen fertility

Comparison of extraembryonic expression of Eomes and Cdx2 in pregastrulation chick and mouse embryo unveils regulatory changes along evolution

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