Abstract. Results of background measurements with a prototype of the Borexino detector were used to search for 478 keV solar axions emitted in the M1-transitions of 7 Li * . The Compton conversion of axion to a photon A + e → e + γ, axioelectric effect A + e + Z → e + Z, decay of axion in two photons A → 2γ and Primakoff conversion on nuclei A + Z → γ + Z are considered. The upper limit on constants of interaction of axion with electrons, photons and nucleons -g Ae g AN ≤ (1.0-2.4) × 10 −10 at m A ≤ 450 keV and g Aγ g AN ≤ 5 × 10 −9 GeV −1 at m A ≤ 10 keV are obtained (90%c.l.). For heavy axions with mass at 100 < m A < 400 keV the limits g Ae < (0.7-2.0) × 10 −8 and g Aγ < 10 −9 -10 −8 are obtained in assumption that g AN depends on m A as for KSVZ axion model. These limits are stronger than obtained in previous laboratory-based experiments using nuclear reactor and artificial radioactive sources.