Search for Molecular Markers of Wheat  Resistance to Fungal Pathogens

Yarullina, L. G.; Веселова, С. В.; Ibragimov, R. I.; Shpirnaya, Irina; Kasimova, R. I.; Akhatova, A. R.; Tsvetkov, V. O.; Максимов, И. В.

doi:10.4236/as.2014.58076

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…Figure 3. Microscopic analysis of the morphology of P.infestans isolated from potato tuber: a) lemon-shaped sporangium; b) chlamydospore; c) amphiginous antheridium and oogonium (28 days) An artificial re-infection method was used to identify the virulence of the causative fungus (Yarullina et al, 2016). Healthy potato tubers of the Picasso sort infected with the fungus P.infestans and incubated infected buds at 24 °C temperature for 21 days.…”

Section: Resultsmentioning

confidence: 99%

Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

Azimova,

Khalilov,

Kobilov

et al. 2024

Pak. J. Phytopathol.

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An isolate belonging to the genus Phytophthora was isolated from samples of infected potato plants in Uzbekistan. The morphological characteristics of the isolate were studied and the fungus Ph. infestans belonging to the type of oomycetes was determined. The colony of Phytophthora. sp isolate is colorless and the mycelium appears porous when growing. When studying the microscopic appearance, it was found that the hyphae are not septated, the width of the hypha is 2.5-4.4 μm, the width of the sporangium is 12-17 μm, and the length is 18-25 μm. It was found that P.infestans has amphigynous antheridium with oogonium and chlamydospores. To determine the exact phylogenetic status of this isolate, rRNA gene ITS region (845 bp) and cox2 (627 bp) gene were PCR amplified. The phylogeny of both marker genes showed that the isolate was Ph. Infestans and named Ph. infestans TVKT-1 due to its closeness to Ph. infestans species. The analysis of the ITS region of the rDNA gene as a barcode alone does not provide enough information for the identification of species belonging to the genus Phytophthora, for this, it is important to study the genetic analysis of oomycetes with the additional study of the cox2 gene. Compared to Phytophthora infestans species in the NCBI database, Ph. infestans TVKT-1, which we isolated, was found to have SNP mutations in the ITS region (16, 23, and 29 nucleotides) and cox2 gene (362, 364, and 365 nucleotides) nucleotide sequences.

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Section: Resultsmentioning

confidence: 99%

Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

Azimova,

Khalilov,

Kobilov

et al. 2024

Pak. J. Phytopathol.

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“…Elucidating the differential expression of such genes in resistant and susceptible varieties could contribute to novel strategies in markerassisted breeding of crops with improved tolerance to biotic and abiotic stresses (Feldman et al 2014;Yarullina et al 2014). In the present study we monitored the expression of a published T. aestivum Bowman-Birk type proteinase inhibitor WALI3 under drought and recovery.…”

Section: Discussionmentioning

confidence: 99%

Transcript profiling of serine- and cysteine protease inhibitors inTriticum aestivumvarieties with different drought tolerance

Vaseva

Zehirov

Kirova

et al. 2016

Cereal Research Communications

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A high number of protease inhibitors (PI) have been identified in diverse plant species but information about their role in plant stress responses is still fragmentary. Transcript profiling of six published serine and cysteine protease inhibitor sequences in water-deprived plants from four winter wheat (Triticum aestivum) varieties with varying tolerance was performed in order to outline PIs predominantly accumulating under drought. Expression was analyzed by real time RT-qPCR. Considerable transcript accumulation of Bowman-Birk type PI WALI3 (BBPI) was detected in drought stressed leaves suggesting an important regulatory role of BBPI in adjustment of protein metabolism in leaves under dehydration. Serpin transcripts were less represented in water-deprived plants. Transient accumulation of cystatin transcripts revealed organ-specificity. Under drought cystatin and serpin expression in the leaves of the most drought tolerant variety "Katya" tended to preserve relatively stable levels close to the controls. This preliminary data will serve for future detailed study of regulation of proteolysis in winter wheat subjected to unfavorable environmental factors for development of molecular-based strategies for selection of tolerant varieties.

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“…У каждого сорта оценивали по 10 листьев в 3-кратной повторности. Учет болезни проводили однократно при четких проявлениях септориозных пятнистостей и до начала естественного отмирания листьев [20]. Этот метод особенно актуален при недостатке инфекционного биоматериала и массовой оценке генофонда.…”

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Comparative analysis of methods of assessing spring soft wheat for resistance to <I>Parastagonospora nodorum</I> and search for sources of a trait for breeding

Харина

Шешегова

2022

Аграрная наука Евро-Северо-Востока

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In the conditions of Kirov region (2017-2021), 67 varieties of spring soft wheat were studied according to the degree of resistance to Septoria leaf blotch in the field against the background of the natural development of infection. Under laboratory conditions, the reaction of these wheat varieties to the pathogen was evaluated at the beginning of plant ontogenesis (juvenile resistance) in two experiments: assessment in the development phase of 2-3 leaves (roll culture) and evaluation of a trait on cut-off leaves. For inoculation of plants, local strains of Р. nodorum were used. They were isolated from the affected leaves of spring soft wheat in the phase of milk-wax ripeness. All isolates have been identified by PCR as belonging to the species of Р. nodorum. The validity of using laboratory methods of plant inoculation by Р. nodorum for mass assessment of the gene pool and purposeful search for wheat genotypes resistant to Septoria is based on a reliable (at P≥0.95) correlation (r = 0.57) of resistance at the beginning of ontogenesis and in adult plants. Varieties belonging to one and the same groups in terms of resistance and susceptibility have an even closer relationship (r = 0.69; r = 0.71). It has been established that with an increase in the degree of affection at the beginning of ontogenesis, the biomass of the plant significantly (r = -0.68) decreases, on average by 28 % in the assortment. Of particular interest for breeding of spring wheat are varieties with the most favorable combination of low infection rate and resistance to Septoria leaf blotch during ontogenesis. These varieties include: Turinskaya, Egisar 29 (Russia), Mian Yong No.1, Yan Shi 4, Jin Mai 71 (China), SSL 25-26, SSL 84-85, UL Alta Blanca (USA).

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Search for Molecular Markers of Wheat Resistance to Fungal Pathogens

Cited by 7 publications

References 28 publications

Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

Illustration of Morphological Features and Early Molecular Identification of P. Infestans in Uzbekistan

Transcript profiling of serine- and cysteine protease inhibitors inTriticum aestivumvarieties with different drought tolerance

Comparative analysis of methods of assessing spring soft wheat for resistance to <I>Parastagonospora nodorum</I> and search for sources of a trait for breeding

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