Search for Microsatellite Markers Associated with Water-Stress Tolerance in Wheat Through Bulked Segregant Analysis

Altinkut, Ahu; Gözükırmızı, Nermin

doi:10.1385/mb:23:2:97

Cited by 22 publications

(17 citation statements)

References 10 publications

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“…However, once microsatellite markers are found linked to the target locus, they can be efficiently used to transfer this information to reference maps and unrelated pedigrees and used as starting points for positional cloning efforts. With a more extensive availability of microsatellite markers in several crop plants, these markers have increasingly been used in BSA experiments [1,2,27].…”

Section: Resultsmentioning

confidence: 99%

Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis

Missiaggia

Piacezzi

Grattapaglia

2005

Tree Genetics & Genomes

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An early flowering mutant plant of Eucalyptus grandis with normal vegetative growth was found in a nursery in northern Brazil. This mutant plant flowers at approximately 90 days from germination. A cross between a wild-type (normal flowering) tree and the mutant was carried out, generating a progeny of 88 individuals where early flowering segregated in an approximate 1:1 ratio. A genome scan with 100 microsatellite markers distributed across the genome was carried out using bulk segregant analysis (BSA) on two contrasting bulks of 15 plants each. Linkages (LOD>3.0) with a major effect early flowering quantitative trait locus (QTL) were detected and confirmed by a full scale cosegregation analysis for markers EMBRA27, EMBRA60, EMBRA164, EMBRA158, EMBRA91, and EMBRA65. A localized linkage map involving the six loci and the early flowering QTL named Eucalyptus early flowering 1 (Eef1) was constructed belonging to linkage group #2 in the existing microsatellite reference map. The Eef1 locus was mapped between markers EMBRA27 and EMBRA164, with distances of 21.8 and 6.4 cM, respectively. In introgression experiments, these two markers could be successfully used with an expected precision of 98% to select plants carrying the Eef1 mutant allele, assuming no recombination interference in the genomic segment. Early flowering could be a very useful trait both in breeding as well as experimental genetics of Eucalyptus.

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Section: Resultsmentioning

confidence: 99%

Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis

Missiaggia

Piacezzi

Grattapaglia

2005

Tree Genetics & Genomes

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“…Though the potential of BSA has been demonstrated to tag genes controlling qualitative traits, the method could now be extended for analysis of complex traits such as drought resistance. Markers linked to drought-resistance traits have been identified using BSA in barley [26], wheat [27] and maize [28]. Markers linked to yield [29] and yield under drought stress in rice [24] have also been identified using BSA.…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping QTL for Drought Resistance Traits in Rice (Oryza sativa L.) Using Bulk Segregant Analysis

et al. 2011

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Drought stress is a major limitation to rice (Oryza sativa L.) yields and its stability, especially in rainfed conditions. Developing rice cultivars with inherent capacity to withstand drought stress would improve rainfed rice production. Mapping quantitative trait loci (QTLs) linked to drought resistance traits will help to develop rice cultivars suitable for water-limited environments through molecular marker-assisted selection (MAS) strategy. However, QTL mapping is usually carried out by genotyping large number of progenies, which is labour-intensive, time-consuming and cost-ineffective. Bulk segregant analysis (BSA) serves as an affordable strategy for mapping large effect QTLs by genotyping only the extreme phenotypes instead of the entire mapping population. We have previously mapped a QTL linked to leaf rolling and leaf drying in recombinant inbred (RI) lines derived from two locally adapted indica rice ecotypes viz., IR20/Nootripathu using BSA. Fine mapping the QTL will facilitate its application in MAS. BSA was done by bulking DNA of 10 drought-resistant and 12 drought-sensitive RI lines. Out of 343 rice microsatellites markers genotyped, RM8085 co-segregated among the RI lines constituting the respective bulks. RM8085 was mapped in the middle of the QTL region on chromosome 1 previously identified in these RI lines thus reducing the QTL interval from 7.9 to 3.8 cM. Further, the study showed that the region, RM212-RM302-RM8085-RM3825 on chromosome 1, harbours large effect QTLs for drought-resistance traits across several genetic backgrounds in rice. Thus, the QTL may be useful for drought resistance improvement in rice through MAS and map-based cloning.

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“…Since water-stress tolerance is a quantitatively inherited trait, controlled by several genetic loci, which are difficult to measure (Forster et al, 2000), identification of molecular markers associated with a major locus contributing to water-stress tolerance would be useful for indirect selection for water-stress tolerance (Altinkut and Gozukirmizi, 2003). However, in most instances, identifying genetic markers associated with important genes or traits requires screening of a relatively large number of individuals in the population (Lawson et al, 1994).…”

Section: Resultsmentioning

confidence: 99%

AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)

Altinkut¹,

Kazan

Gözükırmızı

2003

Genet. Mol. Biol.

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The amplified fragment length polymorphism (AFLP) assay is an efficient method for the identification of molecular markers, useful in the improvement of numerous crop species. Bulked Segregant Analysis (BSA) was used to identify AFLP markers associated with water-stress tolerance in barley, as this would permit rapid selection of water-stress tolerant genotypes in breeding programs. AFLP markers linked to water-stress tolerance was identified in two DNA pools (tolerant and sensitive), which were established using selected F 2 individuals resulting from a cross between water-stress-tolerant and sensitive barley parental genotypes, based on their paraquat (PQ) tolerance, leaf size, and relative water content (RWC). All these three traits were previously shown to be associated with water-stress tolerance in segregating F 2 progeny of the barley cross used in a previous study. AFLP analysis was then performed on these DNA pools, using 40 primer pairs to detect AFLP fragments that are present/absent, respectively, in the two pools and their parental lines. One separate AFLP fragment, which was present in the tolerant parent and in the tolerant bulk, but absent in the sensitive parent and in the sensitive bulk, was identified. Polymorphism of the AFLP marker was tested among tolerant and sensitive F 2 individuals. The presence of this marker that is associated with water-stress tolerance will greatly enhance selection for paraquat and water-stress tolerant genotypes in future breeding programs.

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Search for Microsatellite Markers Associated with Water-Stress Tolerance in Wheat Through Bulked Segregant Analysis

Cited by 22 publications

References 10 publications

Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis

Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis

Fine Mapping QTL for Drought Resistance Traits in Rice (Oryza sativa L.) Using Bulk Segregant Analysis

AFLP marker linked to water-stress-tolerant bulks in barley (Hordeum vulgare L.)

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