Search for beneficial bacterial strains for turbot (Scophthalmus maximus L.) larviculture

Huys, Liesbeth; Dhert, Philippe; Robles, R.; Ollevier, Frans; Sorgeloos, Patrick; Swings, Jean

doi:10.1016/s0044-8486(00)00474-9

Cited by 64 publications

(40 citation statements)

References 20 publications

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“…Whether these facts could be related to a protective or beneficial role against infection by other bacteria, as suggested for other Vibrio spp. (Huys et al 2001), needs further confirmation.A relatively low percentage of fish carried a Vibrio sp. LB+ (lysine decarboxylase and β-hydroxybutyrate+), different from all the known Vibrio spp.…”

mentioning

confidence: 91%

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

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A bacteriological survey of gilthead sea bream Sparus aurata from different fish farms and culture systems on the Spanish Mediterranean coast was conducted. Three different studies were performed. Study A included hatchery-reared larvae; Study B, periodic examination of randomly sampled growing fish; and Study C, growing fish sampled only during mortality/morbidity events. In Studies B and C, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical signs) and non-diseased fish were included. In Study A, a shift from Vibrio spp. (30 d after hatching) to oxidative species (60 d after hatching) was detected, and no mortality events were registered. The percentage of fish yielding bacterial growth were similar in Studies B and C, reaching 57.4 and 61.3%, respectively. A statistically significant relationship between the bacterial carriage and the type of facility was only found in Study B, showing that fish from sea cages had a higher bacterial occurrence than fish from other facilities. A statistically significant relationship between bacterial carriage and signs of disease was found, although the pattern differed in each study. Thus, in Study B only 36.2% of fish yielding abundant bacterial growth were diseased, versus 68.0% in Study C. In total, 25.0% of the fish examined were diseased. Bacterial species composition was similar in asymptomatic and diseased fish, except for a group of V. ichthyoenteri-like isolates that occurred almost exclusively in asymptomatic fish. Dominant bacterial species were V. harveyi and V. splendidus, followed by V. ichthyoenteri-like isolates, Photobacterium damselae ssp. damselae and V. fisheri. Non-fermenters were less frequent but, among them, unidentified halophilic Cytophaga-Flavobacterium isolates and Pseudoalteromonas haloplanktis were the most abundant. An association of individual species with disease was not clear, which suggests the involvement of mixed infections. KEY WORDS: Sparus aurata · Vibrio harveyi · Vibrio splendidus · Photobacterium damsela · Pseudoalteromonas haloplanktis · Vibrio ichthyoenteri · Mediterranean aquaculture Resale or republication not permitted without written consent of the publisherDis Aquat Org 54: [119][120][121][122][123][124][125][126] 2003 Baptista et al. 1999). These species have been recovered from moribund or recently dead fish showing clinical signs. However, studies on the bacterial flora present in asymptomatic fish are scarce.Several studies have been conducted along the Spanish Mediterranean coast to identify the main bacterial groups present in water and bivalves (Ortigosa et al. 1994a,b, Arias et al. 1999. Some of these bacteria exhibit clear seasonal variation, as Vibrio harveyi dominated with temperatures above 20°C and V. splendidus dominated at temperatures below 20°C (Arias et al. 1999). These and other bacterial species showing seasonal occurrence have been reported as opportunistic pathogens for sea bass and sea bream (Balebona 1998b).In the present study, bacterial car...

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confidence: 91%

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Pujalte¹,

Sitjà‐Bobadilla²,

Álvarez-Pellitero³

et al. 2003

Dis. Aquat. Org.

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“…Among other options (such as immunostimulation), the use of probiotics is receiving considerable attention (3,15,18,22,34,52,53). The application of a single probiotic strain or a combination of strains either in the rearing water or in live feed aims at a permanent or transient beneficial colonization of the host.…”

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confidence: 99%

Effects of Bacteria on Artemia franciscana Cultured in Different Gnotobiotic Environments

Marques

Dinh

Ioakeimidis

et al. 2005

Appl Environ Microbiol

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The use of probiotics is receiving considerable attention as an alternative approach to control microbiota in aquaculture farms, especially in hatching facilities. However, application with consistent results is hampered by insufficient information on their modes of action. To investigate whether dead bacteria (allowing investigation of their nutritional effect) or live bacteria (allowing evaluation of their probiotic effect) have any beneficial effect towards Artemia franciscana and, subsequently, if live bacteria have probiotic effects beyond the effects observed with dead bacteria, a model system was employed using gnotobiotic Artemia as a test organism. Nauplii were cultured in the presence of 10 bacterial strains combined with four different major axenic live feeds (two strains of Saccharomyces cerevisiae and two strains of Dunaliella tertiolecta) differing in their nutritional values. In combination with poor-and medium-quality live feeds, dead bacteria exerted a strong effect on Artemia survival but a rather weak or no effect on individual length and constituted a maximum of only 5.9% of the total ash-free dry weight supplied. These effects were reduced or even disappeared when mediumto good-quality major feed sources were used, possibly due to improvements in the health status of Artemia. Some probiotic bacteria, such as GR 8 (Cytophaga spp.), improved (not always significantly) the performance of nauplii beyond the effect observed with dead bacteria, independently of the feed supplied. The present approach can be an excellent system to study the exact mode of action of bacteria, especially if combined with challenge tests or other types of analysis (e.g., transcriptome and proteonomic analysis).

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“…Twenty-two isolates [LMG 19839 T (l CAIM 430 T ), LMG 19840 (l CAIM 427), LMG 19841 ( l CAIM 436), LMG 19842 (l CAIM 451), LMG 20957 (l CAIM 428), R-3719, R-3668, R-3678, R-3708, R-3717, R-3727, R-3729, R-3731, R-3749, R-3759, R-3764, R-3929, R-3773, R-3792, R-3814, R-3819, R-3847], isolated from the gut of healthy turbot larvae at the Aquaculture Research Station of Austevoll (Norway) during the summer of 1997 as described previously (Huys et al, 2001), were analysed. All strains included in this study were deposited in the BCCM\LMG Bacteria Collection at Ghent University and in the CAIM collection of the Centre of Research on Nutrition and Development (CIAD) in Mazatla!…”

Section: Methodsmentioning

confidence: 99%

“…1 (Oxoid) and with 1n5% (w\v) NaCl for 24 h at 28 mC. Fatty acid methyl ester analysis was carried out as described by Huys et al (2001). Isolates were grown on trypticase soy broth (Becton Dickinson) supplemented with 1n5% (w\v) Bacto agar (Becton Dickinson) and 1n5% (w\v) NaCl and on MA at 28 mC for 24 h. Approximately 50 mg cells was harvested and the fatty acids were isolated and analysed using the Microbial Identification System software package, version 3.9 (Microbial ID).…”

Section: Methodsmentioning

confidence: 99%

“…Mortalities in early larval stages of intensively cultured marine fish are often very high and related to bacterial infections in which opportunistic bacteria play an important role (Ishimura et al, 1996 ;Diggles et al, 2000). On the other hand, it has also been proved that certain Vibrio strains isolated from the gut of S. maximus larvae, when added to the culture water, improve larval survival and growth (Huys et al, 2001). Culturable microflora from the gut of fish larvae have been the subject of many reports, but these microorganisms have generally been identified only at the family and\or genus level Ring & Gatesoupe, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (Scophthalmus maximus) larvae: a new member of the family Vibrionaceae.

Thompson¹,

Hoste²,

Thompson³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Search for beneficial bacterial strains for turbot (Scophthalmus maximus L.) larviculture

Cited by 64 publications

References 20 publications

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms

Effects of Bacteria on Artemia franciscana Cultured in Different Gnotobiotic Environments

Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot (Scophthalmus maximus) larvae: a new member of the family Vibrionaceae.

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