SEARCH ASSOCIATION OF POLYMORPHISM rs3918242 IN MMP9 GENE WITH GUM RECESSION OF CHILDREN

Abstract

Subject. Gingival recession is a complex multifactorial disease, the development of which is influenced by the interaction of many genes and environmental factors (orthodontic and mucogingival anomalies, bad habits, muscle hypertonicity). Since, in addition to environmental factors, genes also influence the development of this pathology, the study of the molecular genetic basis of gum recession is an urgent task of modern dentistry. Such studies can make a decisive contribution to the creation of a concept describing the development of gingival recession, will help determine the prospects for its personalization and will allow the development of scientifically based comprehensive programs for the prevention of periodontal pathology.
 The aim is to study the association of the rs3918242 of the metalloproteinase 9 (MMP9) gene in children with the development of gum recession citizensof the Republic of Tatarstan.
 Methodology. The study sample included 284 patients, of which 200 relatively healthy ones constituted the control group (intact periodontium) and 84 - the observation group (gingival recession). DNA was isolated from buccal epithelial cells. Further genotyping of the rs3918242 polymorphism of the MMP9gene was performed using real-time polymerase chain reaction on a CFX96 amplifier (BioRad, USA) in accordance with the manufacturer's instructions (Sib-DNA, Novosibirsk). Statistical analysis was performed to assess the differences between the study groups.
 Results. There were no statistically significant differences in the frequency of alleles and genotypes of the rs3918242 polymorphism of the MMP9 gene between the study groups (p > 0.05).
 Conclusions. The role of the rs3918242 polymorphism of the MMP9 gene in the development of gum recession in children living in the Republic of Tatarstan has not been established using the case-control methodological approach.