Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel

Wu, Hongwei; Shang, Yuna; Sun, Wei; Ouyang, Xinyi; Zhou, Wenyan; Lu, Jieji; Wei, Wei; Yao, Xudong; Wang, Xiaozhao; Zhang, Xianzhu; Chen, Yishan; He, Qun; Yang, Zhimou; Ouyang, Hongwei

doi:10.1016/j.bioactmat.2022.03.038

Cited by 21 publications

(16 citation statements)

References 81 publications

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“…It is more attractive as a precursor for hydrogels preparation due to its processability, superior water solubility, and lower immunogenicity compared to collagen [ [39] , [40] , [41] ]. Nonetheless, pure gelatin has a faster degradation rate and poorer mechanical properties, often falling short of expectations [ 42 ]. Hyaluronic acid（HA）, a polysaccharide-based polymer consisting of a disaccharide unit composed of glucuronic acid and N-acetyl glucosamine, exists in soft tissue and synovial fluid, interacts with the extracellular matrix of cartilage and plays an essential role in mesenchymal cell migration, cohesion, cartilage differentiation and maintenance of intrachondral homeostasis [ [43] , [44] , [45] ].…”

Section: Discussionmentioning

confidence: 99%

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

Lü

Zeng

Liu

et al. 2023

Materials Today Bio

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Section: Discussionmentioning

confidence: 99%

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

Lü

Zeng

Liu

et al. 2023

Materials Today Bio

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“…Similar results have been seen in other animal models. 29,47,50 This integration of gaps in articular cartilage is a persistent challenge noted in larger osteochondral repairs. Lack of integration may lead to formation of fibrotic tissues and leaves the joint surface vulnerable to further damage.…”

Section: Discussionmentioning

confidence: 99%

Novel Osteochondral Biotemplate Improves Long-term Cartilage Repair Compared With Microfracture in an Ovine Model

Dickerson,

Fortier,

Nauman

et al. 2023

Am J Sports Med

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Background: Current cartilage repair therapies do not re-create the complex mechanical interface between cartilage and bone, which is critical for long-term repair durability. New biomaterial designs that include hard tissue–soft tissue interface structures offer promise to improve clinical outcomes. Purpose/Hypothesis: The purpose of this study was to evaluate the efficacy and safety of a naturally derived osteochondral biotemplate with a novel contiguous hard tissue–soft tissue interface in an ovine model as a regenerative solution for articular cartilage defects. It was hypothesized that the osteochondral biotemplate would produce structurally superior repair tissue compared with microfracture over a 13-month period. Study Design: Controlled laboratory study. Methods: Osteochondral biotemplates were manufactured from porcine cancellous bone. Skeletally mature sheep (N = 30) were randomly allocated to 3 groups: early healing stage (euthanasia at 4 months), 6-month treatment, and 13-month treatment. In the early healing stage group, an 8 mm–diameter by 5 mm–deep osteochondral defect was created on the medial femoral condyle and treated at the time of iatrogenic injury with an osteochondral biotemplate. The contralateral limb received the same treatment 2 months later. In the 6- and 13-month treatment groups, 1 limb received the same osteochondral procedure as the early healing stage group. In the contralateral limb, an 8 mm–diameter, full-thickness cartilage defect (1-2 mm deep) was created and treated with microfracture. Cartilage repair and integration were quantitatively and qualitatively assessed with gross inspection, histological evaluation, and magnetic resonance imaging (MRI). Wilcoxon signed-rank and McNemar tests were used to compare the treatments. Results: At 6 and 13 months after treatment, the biotemplate was not present histologically. At 13 months, the biotemplate treatment demonstrated statistically higher histological scores than microfracture for integration with surrounding cartilage (biotemplate: 74 ± 31; microfracture: 28 ± 39; P = .03), type 2 collagen (biotemplate: 72 ± 33; microfracture: 40 ± 38; P = .02), total cartilage (biotemplate: 71 ± 9; microfracture: 59 ± 9; P = .01), and total integration (biotemplate: 85 ± 15; microfracture: 66 ± 20; P = .04). The osteochondral biotemplate treatment produced a notable transient nonneutrophilic inflammatory response that appeared to approach resolution at 13 months. MRI results were not statistically different between the 2 treatments. Conclusion: Even with the inflammatory response, after 13 months, the osteochondral biotemplate outperformed microfracture in cartilage regeneration and demonstrated superiority in integration between the repair tissue and host tissue as well as integration between the newly formed cartilage and the underlying bone. Clinical Relevance: This work has demonstrated the clinical potential of a novel biomaterial template to regenerate the complex mechanical interface between cartilage and the subchondral bone.

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“…Biomolecules, such as growth factors, cytokines, peptides, small molecule drugs, etc., play a key role in regulating cell behavior (such as migration, proliferation, differentiation, and ECM formation). In cartilage and osteochondral tissue engineering, they can promote the differentiation of progenitor cells into chondrocytes (such as TGF-β1, IGF-1, and KGN), − promote the proliferation of chondrocytes (such as FGF, EGF), , and promote matrix synthesis (such as BMP-2, -4, -6, and -7, IGF-1, TGF-1, TGF-β1), − etc. Some biomolecules play an important role in controlling inflammation (such as aspirin, acetaminophen, and ibuprofen), which showed therapeutic effects in the treatment of inflammatory diseases such as OA.…”

Section: Additives In Photo-cross-linked Hydrogelsmentioning

confidence: 99%

Photo-cross-linked Hydrogels for Cartilage and Osteochondral Repair

Zhu,

Chen,

Liu

et al. 2023

ACS Biomater. Sci. Eng.

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Photo-cross-linked hydrogels, which respond to light and induce structural or morphological transitions, form a microenvironment that mimics the extracellular matrix of native tissue. In the last decades, photo-cross-linked hydrogels have been widely used in cartilage and osteochondral tissue engineering due to their good biocompatibility, ease of fabrication, rapid in situ gel-forming ability, and tunable mechanical and degradable properties. In this review, we systemically summarize the different types and physicochemical properties of photo-cross-linked hydrogels (including the materials and photoinitiators) and explore the biological properties modulated through the incorporation of additives, including cells, biomolecules, genes, and nanomaterials, into photo-cross-linked hydrogels. Subsequently, we compile the applications of photo-cross-linked hydrogels with a specific focus on cartilage and osteochondral repair. Finally, current limitations and future perspectives of photo-cross-linked hydrogels are also discussed.

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Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel

Cited by 21 publications

References 81 publications

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

Novel Osteochondral Biotemplate Improves Long-term Cartilage Repair Compared With Microfracture in an Ovine Model

Photo-cross-linked Hydrogels for Cartilage and Osteochondral Repair

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