Sealing of open dentinal tubules by laser irradiation: AFM and SEM observations of dentine surfaces

Kubínek, Roman; Zapletalová, Zdeňka; Vůjtek, Milan; Novotný, Radko; Kolářová, Hana; Chmelíčková, Hana; Peřina, Jan

doi:10.1002/jmr.856

Cited by 15 publications

(12 citation statements)

References 29 publications

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“…To solve this problem, the dentin surface may be covered with an aqueous agent that is capable of absorbing the radiation, causing its own temperature to increase instead of that of dentin. Kubínek et al recommended that the surface of dentin be covered with a dye that absorbs laser radiation (Kubínek et al, ). In Groups 6 and 7, Gluma or Teethmate desensitizer was applied to the dentin surface prior to the application of the laser, this method may be more advantageous than the use of laser or desensitizer alone because the rates of dentinal tubule closure were higher in these groups (0%, 86.2%, 61.6%, 90,8%, 91,6%, 96.2%, and 93.3% dentinal tubule closure in Groups 1, 2, 3, 4, 6, and 7, respectively).…”

Section: Discussionmentioning

confidence: 99%

Effects of different desensitizers and lasers on dentine tubules: An in‐vitro analysis

Öncü

Karabekiroğlu

Ünlü

2017

Microscopy Res & Technique

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Section: Discussionmentioning

confidence: 99%

Effects of different desensitizers and lasers on dentine tubules: An in‐vitro analysis

Öncü

Karabekiroğlu

Ünlü

2017

Microscopy Res & Technique

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“…For investigations of biological specimens, the tapping mode is preferred to avoid surface damage caused by probing friction [37]. Because the fine polishing powders used in the present study were of small diameters (1.00 μm, 0.30 μm and 0.05 μm), and the mean diameter of the dentinal tubules from human third molars in young adults was reported to be approximately 1.21 (0.08) μm [38], it was critical to place the specimens in an ultrasonic cleaner for 30 min before commencing the AFM observations.…”

Section: Discussionmentioning

confidence: 99%

Long-Term In Vitro Effectiveness of A Bioglass Desensitizing Agent Investigated Using Electrochemical Impedance Spectroscopy, Atomic Force Microscopy and Scanning Electron Microscopy

Shi¹,

Wu²,

Xu³

et al. 2017

Dentistry

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Objectives: To compare the long-term in vitro effectiveness of a newer desensitizing agent containing bioactive glass and two other commercial products employed for dentin hypersensitivity.Methods: Fifty occlusal dentin disks from extracted sound human third molars were treated with 0.5M ethylenediaminetetraacetic acid for 2 min then randomly divided into five groups (n=10). Specimens were brushed for 2 min twice daily with Actimins Paste (Group1), Lesening Super Desensitizing Toothpaste (Group2), Colgate Sensitive Pro-Relief Desensitising Polishing Paste (Group3) and distilled water (Group4) at 9:00 am and at 5:00 pm. Specimens in Group5 had no brushings. All specimens were immersed sequentially for 5 min into coffee (pH=5.4) at 10:00 am and undiluted cola (pH=2.5) at 6:00 pm. Dentin permeability was measured weekly over one month using electrochemical impedance spectroscopy (EIS). Data were analyzed using two-way repeated measures ANOVA and post hoc LSD tests. Dentin microstructures were observed at one month by atomic force microscopy and scanning electron microscopy. Results:The three desensitizing agents significantly reduced dentin permeability over one month. Post hoc LSD tests of the EIS values indicated that brushing with Actimins showed no significant difference from Colgate Sensitive (P=0.32), and that both agents had significantly less permeability than Lesening (P<0.05). Conclusions:The three desensitizing agents were effective in occluding dentinal tubules and reducing permeability to varying extents over one month, with Actimins and Colgate Sensitive being the most effective. Actimins might be initially, a faster-working desensitizing agent.Clinical significance: Actimins, a newer desensitizing agent containing bioactive glass, has the potential to relieve dentin hypersensitivity in the presence of long-term daily acidic beverage ingestion.

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“…TSM also offers application for in vivo imaging. Other imaging methods include environmental SEM if available, although this technique does not allow samples to be imaged completely wet and requires a laboratory setting [Kubinek et al, 2007]. The SEM remains of use for the visual ordinal 'standard' assessment, as shown in the calibrations.…”

Section: Discussionmentioning

confidence: 99%

A Novel Method to Quantify Dentine Tubule Occlusion Applied to in situ Model Samples

Olley

Parkinson

Wilson³

et al. 2013

Caries Res

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Objectives: To develop an innovative computerised routine, in conjunction with a minimally destructive imaging technique, to quantify dentine tubule occlusion. Methods: Polished human dentine samples (n = 480) were brushed during a 4-day in situ study with two occlusion-based dentifrices, a sodium fluoride control dentifrice (1,450 ppm) or water. Samples were imaged with tandem scanning microscopy (TSM) and conventional scanning electron microscopy (SEM). The level of dentine patency was then assessed using a visual ordinal scale (‘standard') or quantitatively using a specially designed computational routine. Results: The occlusion-based dentifrice resulted in significantly less patent dentine than controls for the ‘standard' (p = 0.01) assessment, but not for computer analyses (p = 0.10). The correlation of the number of individual patent tubules counted visually and by the computational routine in calibrations and in the in situ study was ≥0.8. Conclusions: This study identified a new computer-based routine, capable of objectively quantifying the patency of dentine imaged by SEM and TSM.

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Sealing of open dentinal tubules by laser irradiation: AFM and SEM observations of dentine surfaces

Cited by 15 publications

References 29 publications

Effects of different desensitizers and lasers on dentine tubules: An in‐vitro analysis

Effects of different desensitizers and lasers on dentine tubules: An in‐vitro analysis

Long-Term In Vitro Effectiveness of A Bioglass Desensitizing Agent Investigated Using Electrochemical Impedance Spectroscopy, Atomic Force Microscopy and Scanning Electron Microscopy

A Novel Method to Quantify Dentine Tubule Occlusion Applied to in situ Model Samples

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