Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recording

Regehr, Wade G.; Pine, J.; Cohan, Christopher S.; Mischke, Michelle; Tank, David W.

doi:10.1016/0165-0270(89)90055-1

Cited by 176 publications

(111 citation statements)

References 21 publications

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“…For electrical measurements, high source impedance signals from the electrodes are transferred through a 20 cm cable to 11X non-inverting pre-amplifiers (Regehr et al, 1989), then to 2X non-inverting buffer amplifiers with a three-pole low pass filters (3 dB point at 5 kHz). The signals are then passed to two 8-channel 16 bit A/D converters, with 8X amplifiers and 9 10 V range (National Instruments) in a Macintosh II personal computer.…”

Section: Electronicsmentioning

confidence: 99%

“…Since the cell bodies are generally pulled to the top of the well by tension generated as the processes mature (Maher et al, 1998), the resistance to ground is approximately R 2 :17.5 kV. The actual shape of the signal can be quite complex (Regehr et al, 1989;Grattarola and Martinoia, 1993), but the largest component occurs when the voltage-gated sodium channels drive the fast depolarization of the membrane of the cell body and dendrites. Measurements of the membrane properties of hippocampal neurons after 7 days in culture, in our laboratory and others (Buchhalter and Dichter, 1991;Yang et al, 1993), show that the cells typically have a membrane capacitance C m : 80 pF and the membrane potential rises at a maximum rate of dV/dt :100 V/s during an action potential.…”

Section: Recordingmentioning

confidence: 99%

“…As a first step towards studying a full network, we demonstrate twoway, non-invasive communication between external electronics and individual neurons cultured in the neurochip. There have been previous demonstrations of related devices for use with individual peripheral mammalian neurons (Pine, 1980), with invertebrate neurons (Regehr et al, 1989;Stett et al, 1997), and with mammalian cell networks (Gross et al, 1977). The neurochip significantly improves on these methods by associating individual electrodes with the cell bodies of each of the neurons in a small network.…”

Section: Introductionmentioning

confidence: 99%

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The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

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304

180

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Section: Electronicsmentioning

confidence: 99%

Section: Recordingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Maher

Pine

Wright

et al. 1999

Journal of Neuroscience Methods

Self Cite

304

180

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“…161 Multiple electrode arrays (MEAs) of up to 100 electrodes patterned by photolithography on glass or silicon substrates have been used for decades; transparent MEAs can be made of indium tin oxide. 162,163 Planar MEAs, consisting of 10-100 electrode sites (with diameters on the order of the cells-i.e., ranging from a few microns up to a few tens of microns) spaced at 100-200 μm interdistance on glass plates, were used by Tomas et al, 164 Gross et al, 165 Novak and Wheeler, 166 and many others followed in studies of the activity and plasticity of developing cultured neuronal networks 167,168 or slices. 162,163,[169][170][171][172][173] MEAs are now commercially available in a variety of formats.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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“…A diversity of shapes and a wide range of amplitudes of signals recorded with planar electrodes from different neurons have been reported (Gross, 1979;Regehr et al, 1989;Bove et al, 1995;Breckenridge et al, 1995;Jenkner & Fromherz, 1997;Schatzthauer & Fromherz, 1998;Fromherz, 1999;Ruardij et al, 2009). Signals were generally classified (arranged in types) according to the waveform and amplitude .…”

Section: Fig 1 Schematic View Of An Electrode Covered With Neuronsmentioning

confidence: 99%

Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity

et al. 2012

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Signals recorded from neurons with extracellular planar sensors have a wide range of waveforms and amplitudes. This variety is a result of different physical conditions affecting the ion currents through a cellular membrane. The transmembrane currents are often considered by macroscopic membrane models as essentially a homogeneous process. However, this assumption is doubtful, since ions move through ion channels, which are scattered within the membrane. Accounting for this fact, the present work proposes a theoretical model of heterogeneous membrane conductivity. The model is based on the hypothesis that both potential and charge are distributed inhomogeneously on the membrane surface, concentrated near channel pores, as the direct consequence of the inhomogeneous transmembrane current. A system of continuity equations having non-stationary and quasi-stationary forms expresses this fact mathematically. The present work performs mathematical analysis of the proposed equations, following by the synthesis of the equivalent electric element of a heterogeneous membrane current. This element is further used to construct a model of the cell-surface electric junction in a form of the equivalent electrical circuit. After that a study of how the heterogeneous membrane conductivity affects parameters of the extracellular electrical signal is performed. As the result it was found that variation of the passive characteristics of the cell-surface junction, conductivity of the cleft and the cleft height, could lead to different shapes of the extracellular signals.

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Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recording

Cited by 176 publications

References 21 publications

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

The neurochip: a new multielectrode device for stimulating and recording from cultured neurons

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity

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