Sea snake Hydrophis cyanocinctus venom. I. Purification, characterization and N-terminal sequence of two phospholipases A2

Ali, Syed Abid; Alam, Junaid Mahmood; Stoeva, Stanka; Schütz, Jürgen; Abbasi, Atiya; Zaidi, Zafar H.; Voelter, Wolfgang

doi:10.1016/s0041-0101(99)00091-4

Cited by 25 publications

(4 citation statements)

References 47 publications

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“…Surprisingly, the heat-denatured extract conserved its lethal effect. In this respect it is worth mentioning that although some thermostable phospholipases A 2 have been isolated from snake venoms (Ali et al, 1999;Wang et al, 2004;Mukherjee, 2007), cytolysins that have been characterized from cnidarians are primarily proteins or polypeptides, whose biological activities are lost at temperatures above 60°C (Anderluh and Macek, 2002). Therefore, it is very likely that the phospholipases A 2 contained in the M. complanata aqueous extract might be responsible for the kidney and lung damage, which could be related to the systemic toxicity due to Millepora spp.…”

Section: Discussionmentioning

confidence: 99%

Systemic toxic effects induced by the aqueous extract of the fire coral Millepora complanata and partial purification of thermostable neurotoxins with lethal effects in mice

García-Arredondo

Rojas‐Molina

Bah

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Discussionmentioning

confidence: 99%

Systemic toxic effects induced by the aqueous extract of the fire coral Millepora complanata and partial purification of thermostable neurotoxins with lethal effects in mice

García-Arredondo

Rojas‐Molina

Bah

et al. 2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…This motif is located in an extra segment of ∼40 amino acids (Figure 2A) that is predicted to be composed mainly of loops and distinguishes TbSP1 from other group XIII PLA 2 s. Interestingly, among the few RGD‐containing proteins thus far identified in fungi, there are the SRAPS of P.tinctorius , which accumulate on the surface of both free‐living and symbiosis‐engaged hyphae and may be involved in ectomycorrhiza formation (Laurent et al ., 1999). Also noteworthy, in this regard, is that the only precedent for an RGD‐containing phospholipase is a sea snake venom ( Hydrophis cyanocinctus ) PLA 2 , in which the RGD motif is thought to mediate interaction with platelet surface receptors, thereby inhibiting platelet aggregation (Ali et al ., 1999).…”

Section: Discussionmentioning

confidence: 99%

A nutrient-regulated, dual localization phospholipase A2 in the symbiotic fungus Tuber borchii

Soragni

Bolchi

Balestrini

et al. 2001

EMBO J

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Important morphogenetic transitions in fungi are triggered by starvation-induced changes in the expression of structural surface proteins. Here, we report that nutrient deprivation causes a strong and reversible up-regulation of TbSP1, a surface-associated, Ca 2+ -dependent phospholipase from the mycorrhizal fungus Tuber borchii. TbSP1 is the ®rst phospholipase A 2 to be described in fungi and identi®es a novel class of phospholipid-hydrolyzing enzymes. The TbSP1 phospholipase, which is synthesized initially as a preprotein, is processed ef®ciently and secreted during the mycelial phase. The mature protein, however, also localizes to the inner cell wall layer, close to the plasma membrane, in both free-living and symbiosisengaged hyphae. It thus appears that a dual localization phospholipase A 2 is involved in the adaptation of a symbiotic fungus to conditions of persistent nutritional limitation. Moreover, the fact that TbSP1-related sequences are present in Streptomyces and Neurospora, and not in wholly sequenced non-®lamen-tous microorganisms, points to a general role for TbSP1 phospholipases A 2 in the organization of multicellular ®lamentous structures in bacteria and fungi.

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“…Phospholipase A2 from the snakes Crotalus durissus terrificus, Bothriechis lateralis, Crotalus durissu sruruima exhibits their activities at 18°C, 30°C and 40°C, respectively [32,33,34]. In contrast, MVL-PLA2 behavior is comparable to many mammalian pancreatic PLA2 [35] and some snake venoms PLA2 such as Hydrophis cyanocinctus and Bothriopsis bilineata PLA2 [36,37].…”

Section: Discussionmentioning

confidence: 99%

Biochemical and monolayer characterization of Tunisian snake venom phospholipases

Baîram

Aissa

Louati

et al. 2016

International Journal of Biological Macromolecules

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The present study investigated the kinetic and interfacial properties of two secreted phospholipases isolated from Tunisian vipers'venoms: Cerastes cerastes (CC-PLA2) and Macrovipera lebetina transmediterranea (MVL-PLA2). Results show that these enzymes have great different abilities to bind and hydrolyse phospholipids. Using egg-yolk emulsions as substrate at pH 8, we found that MVL-PLA2 has a specific activity of 1473U/mg at 37°C in presence of 1mM CaCl2. Furthermore the interfacial kinetic and binding data indicate that MVL-PLA2 has a preference to the zwitterionic phosphatidylcholine monolayers (PC). Conversely, CC-PLA2 was found to be able to hydrolyse preferentially negatively charged head group phospholipids (PG and PS) and exhibits a specific activity 9 times more important (13333U/mg at 60°C in presence of 3mM CaCl2). Molecular models of both CC-PLA2 and MVL-PLA2 3D structures have been built and their electrostatic potentials surfaces have been calculated. A marked anisotropy of the overall electrostatic charge distribution leads to a significantly difference in the dipole moment intensity between the two enzymes explaining the great differences in catalytic and binding properties, which seems to be governed by the electrostatic and hydrophobic forces operative at the surface of the two phospholipases.

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Sea snake Hydrophis cyanocinctus venom. I. Purification, characterization and N-terminal sequence of two phospholipases A2

Cited by 25 publications

References 47 publications

Systemic toxic effects induced by the aqueous extract of the fire coral Millepora complanata and partial purification of thermostable neurotoxins with lethal effects in mice

Systemic toxic effects induced by the aqueous extract of the fire coral Millepora complanata and partial purification of thermostable neurotoxins with lethal effects in mice

A nutrient-regulated, dual localization phospholipase A2 in the symbiotic fungus Tuber borchii

Biochemical and monolayer characterization of Tunisian snake venom phospholipases

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