SDS-PAGE Profile Analysis of SeM-like Protein of Streptococcus equi subspecies equi.

Abstract

S. equi subspecies equi, causing strangles in equine, is characterized by comprising a major virulence factor called M like protein or SeM protein. This study aimed to extract SeM protein from local S. equi strain in Egypt and to detect its antigenic components. After centrifugation, the native 58 kilo Dalton (kDa) SeM protein was detected both in the supernatant and sediment of the prepared extract. With modification by more centrifugation, the formed supernatants were separated and fractionated using SDS-PAGE with silver nitrate staining, which led to the appearance of a band at Molecular Weight (MW) 70.9 kDa.