Scutellarin protects against the liver injury induced by diosbulbin B in mice and its mechanism

Niu, Chengwei; Sheng, Yuchen; Yang, Rui; Lü, Bin; Bai, Qingshun; Ji, Lili; Wang, Zhengtao

doi:10.1016/j.jep.2015.02.031

Cited by 41 publications

(22 citation statements)

References 40 publications

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“…Results of experiments done by Niu et al (2015) demonstrated that scutellarin prevented diosbulbin B (43)-induced liver injury by attenuating NF-κB-mediated hepatic inflammation and ameliorating liver oxidative stress injury. Meanwhile, diosbulbin B combined with scutellarin presents significant anti-tumor activity in vivo.…”

Section: Combination With Scutellaria Barbatamentioning

confidence: 99%

Bioactivity, toxicity and detoxification assessment of Dioscorea bulbifera L.: a comprehensive review

et al. 2017

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Dioscorea bulbifera L. (Chinese: Huangdu, common name: Air potato) is a traditional herbal medicine in China, and it is also one of the most widely consumed yam species, especially in West Africa. Studies have verified that D. bulbifera is effective in treating a wide range of diseases, such as pharyngitis, goitre, pyogenic skin infections, orchitis and cancer. However, more and more studies have also reported liver and kidney damage caused by D. bulbifera. To promote understanding of the bioactivity, toxicity and methods for detoxification of this medicinal and edible plant, the present article reviews the most valuable recent reports on its phytochemistry and pharmacological effects. The possible reasons for its toxicity include the toxic effects of diosbulbin B and D on hepatocytes, the inhibition of antioxidant enzymes in liver mitochondria, and inhibition of enzymes that ordinarily metabolize the herb's components. Synergistic compatibility detoxification may help to reduce toxic effects and improve therapeutic effects. More clinical trials are also required to fully achieve its therapeutic potential.

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Section: Combination With Scutellaria Barbatamentioning

confidence: 99%

Bioactivity, toxicity and detoxification assessment of Dioscorea bulbifera L.: a comprehensive review

et al. 2017

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“…Beyond the NF-κB signaling, the Notch pathway has been identified to be involved in the action of scutellarin on microglia activation ( Yuan et al, 2015 ). Scutellarin has also been shown to attenuate diosbulbin B-induced liver injury by suppressing proinflammatory cytokines ( Niu et al, 2015 ). In clinical practice, scutellarin has been used for the treatment of ischemic cardiovascular and cerebrovascular diseases ( Yuan et al, 2016 ; Gao et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Scutellarin Suppresses NLRP3 Inflammasome Activation in Macrophages and Protects Mice against Bacterial Sepsis

et al. 2018

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The NLRP3 inflammasome plays a critical role in mediating the innate immune defense against pathogenic infections, but aberrant activation of NLRP3 inflammasome has been linked to a variety of inflammatory diseases. Thus targeting the NLRP3 inflammasome represents a promising therapeutic for the treatment of such diseases. Scutellarin is a flavonoid isolated from Erigeron breviscapus (Vant.) Hand.-Mazz. and has been reported to exhibit potent anti-inflammatory activities, but the underlying mechanism is only partly understood. In this study, we aimed to investigate whether scutellarin could affect the activation of NLRP3 inflammasome in macrophages. The results showed that scutellarin dose-dependently reduced caspase-1 activation and decreased mature interleukin-1β (IL-1β) release in lipopolysaccharide (LPS)-primed macrophages upon ATP or nigericin stimulation, indicating that scutellarin inhibited NLRP3 inflammasome activation in macrophages. Consistent with this, scutellarin also suppressed pyroptotic cell death in LPS-primed macrophages treated with ATP or nigericin. ATP or nigericin-induced ASC speck formation and its oligomerization were blocked by scutellarin pre-treatment. Intriguingly, scutellarin augmented PKA-specific phosphorylation of NLRP3 in LPS-primed macrophages, which was completely blocked by selective PKA inhibitor H89, suggesting that PKA signaling had been involved in the action of scutellarin to suppress NLRP3 inflammasome activation. Supporting this, the inhibitory effect of scutellarin on NLRP3 inflammasome activation was completely counteracted by H89 or adenyl cyclase inhibitor MDL12330A. As NLRP3-dependent release of IL-1β has a critical role in sepsis, the in vivo activity of scutellarin was assayed in a mouse model of bacterial sepsis, which was established by intraperitoneally injection of a lethal dose of viable Escherichia coli. Oral administration of scutellarin significantly improved the survival of mice with bacterial sepsis. In line with this, scutellarin treatment significantly reduced serum IL-1β levels and attenuated the infiltration of inflammatory cells in the liver of E. coli-infected mice. These data indicated that scutellarin suppressed NLRP3 inflammasome activation in macrophages by augmenting PKA signaling, highlighting its potential therapeutic application for treating NLRP3-related inflammatory diseases.

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“…Scutellarin isolated from S. barbata reduced cardiomyocyte injury induced by ischemia‐reperfusion through the inhibition of apoptosis and oxidative damage . Scutellarin protected the liver against diosbulbin B‐induced damage by attenuating hepatic inflammation …”

Section: Resultsmentioning

confidence: 99%

“…[27] Scutellarin protected the liver against diosbulbin B-induced damage by attenuating hepatic inflammation. [28] Recently, inhibitory effects of 70% ethanol extract of S. barbata on NO production in Raw264.7 macrophage cells have been reported. [29] Subsequently, the major constituents responsible for the inhibition of NO production in Raw264.7 macrophage cells have been reported as neo-clerodane diterpenoids including scutebarbatine A, B, W, and X, scutebatas B, and 6-O-nicotinolylscutebarbatine G. [30] However, in this study, we first reported that diterpenoids such as scutebarbatine A, B, W, and X, isolated from S. barbata efficiently inhibited NO production in BV2 microglial cells.…”

Section: Inhibitory Effects Of Compounds Isolated From S Barbata On mentioning

confidence: 99%

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS‐Stimulated Microglial Cells

Lee

Kim

et al. 2017

Chemistry & Biodiversity

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The arial parts of Scutellaria barbata D. Don (Lamiaceae) efficiently inhibited NO production in BV2 microglial cells, and the active constituents were further isolated based on activity-guided isolation using silica-gel column chromatography, RP-C18 MPLC and prep-HPLC. As the results, 2 flavonoids including 6-methoxynaringenin (1) and 6-O-methylscutellarein (5), and 6 neo-clerodane diterpenes such as scutebarbatine W (2), scutebatas B (3), scutebarbatine B (4), scutebarbatine A (6), 6-O-nicotinolylscutebarbatine G (7), and scutebarbatine X (8) were isolated. The structures of these compounds were elucidated based on NMR and MS data, and the comparison of literature values. All the compounds except compound 7 inhibited NO production efficiently with IC values of lower than 50 μm. Particularly, compounds 1 and 8 were the most efficient with IC values of 25.8 and 27.4 μm, respectively. This is the first report suggesting the potential of S. barbata on the reduction of neuroinflammation.

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Scutellarin protects against the liver injury induced by diosbulbin B in mice and its mechanism

Cited by 41 publications

References 40 publications

Bioactivity, toxicity and detoxification assessment of Dioscorea bulbifera L.: a comprehensive review

Bioactivity, toxicity and detoxification assessment of Dioscorea bulbifera L.: a comprehensive review

Scutellarin Suppresses NLRP3 Inflammasome Activation in Macrophages and Protects Mice against Bacterial Sepsis

Constituents from Scutellaria barbata Inhibiting Nitric Oxide Production in LPS‐Stimulated Microglial Cells

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