Scrophularia orientalis extract induces calcium signaling and apoptosis in neuroblastoma cells

Lange, Ingo; Moschny, Julia; Tamanyan, Kamilla; Khutsishvili, Manana; Atha, Daniel; Borris, Robert P.; Koomoa, Dana-Lynn T.

doi:10.3892/ijo.2016.3373

Cited by 11 publications

(9 citation statements)

References 45 publications

(32 reference statements)

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“…Baicalin, a phytochemical component of Scutellaria baicalensis Georgi has widespread applications as anti-inflammatory, anti-hepatitis and anti-oxidation agent ( 11 , 20 , 21 ). Furthermore, the anti-cancer effect of baicalin has also been previously documented ( 22 ). The present study aimed to explore the effects of baicalin on ovarian cancer cells and analyze the mechanisms underlining the observed effects.…”

Section: Discussionmentioning

confidence: 87%

Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro

Gao

Zhou

et al. 2017

Molecular Medicine Reports

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Baicalin, an active flavone isolated from Scutellaria baicalensis Georgi, has been demonstrated to induce various beneficial biochemical effects such as anti-inflammatory, anti-viral, and antitumor effects. However, the antitumor mechanism of baicalin is not well understood. In the present study, baicalin was demonstrated to inhibit the viability and migration of a widely used ovarian cancer cell line, A2780, in a dose-dependent manner. MTT assays revealed that cell viability significantly decreased in ovarian cancer cells treated with baicalin compared with untreated cells, without effect on normal ovarian cells. Flow cytometric analysis indicated that baicalin suppressed cell proliferation by inducing apoptosis. The underlying mechanisms involved were indicated to be downregulation of the anti-apoptotic protein B-cell lymphoma 2 apoptosis regulator and activation of caspase-3 and −9. In addition, wound healing and transwell assays revealed that cell migratory potential and expression of matrix metallopeptidase (MMP)-2 and MMP-9 were significantly inhibited when cells were exposed to baicalin, compared with untreated cells. The present study therefore suggested that baicalin has the potential to be used in novel anti-cancer therapeutic formulations for treatment of ovarian cancer.

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Section: Discussionmentioning

confidence: 87%

Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro

Gao

Zhou

et al. 2017

Molecular Medicine Reports

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“…We proved that spilanthol, the main component of the Acmella extract, acted like a potent and natural diuretic in mouse by activating a mechanism based on Ca 2+ -induced inhibition of cytosolic cyclic adenosine monophosphate (cAMP) [ 1 ]. More examples are available in the literature about olive leaf extracts [ 41 ], Scrophularia orientalis [ 42 ], and Echinacea extracts [ 43 , 44 ]. For this latter extract, it has been shown that different bioactive components of Echinacea , such as alkamides (via activation of cannabinoid receptors) [ 43 ] and unknown lipophilic fractions (via direct activation of IP3 receptor) [ 44 ], are able to induce intracellular Ca 2+ peaks.…”

Section: Discussionmentioning

confidence: 99%

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Gerbino

Russo

Colella

et al. 2018

IJMS

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Dandelion (Taraxacum officinale Weber ex F.H.Wigg.) has been used for centuries as an ethnomedical remedy. Nonetheless, the extensive use of different kinds of dandelion extracts and preparations is based on empirical findings. Some of the tissue-specific effects reported for diverse dandelion extracts may result from their action on intracellular signaling cascades. Therefore, the aim of this study was to evaluate the effects of an ethanolic dandelion root extract (DRE) on Ca2+ signaling in human embryonic kidney (HEK) 293 cells. The cytotoxicity of increasing doses of crude DRE was determined by the Calcein viability assay. Fura-2 and the fluorescence resonance energy transfer (FRET)-based probe ERD1 were used to measure cytoplasmic and intraluminal endoplasmic reticulum (ER) Ca2+ levels, respectively. Furthermore, a green fluorescent protein (GFP)-based probe was used to monitor phospholipase C (PLC) activation (pleckstrin homology [PH]–PLCδ–GFP). DRE (10–400 µg/mL) exposure, in the presence of external Ca2+, dose-dependently increased intracellular Ca2+ levels. The DRE-induced Ca2+ increase was significantly reduced in the absence of extracellular Ca2+. In addition, DRE caused a significant Ca2+ release from the ER of intact cells and a concomitant translocation of PH–PLCδ–GFP. In conclusion, DRE directly activates both the release of Ca2+ from internal stores and a significant Ca2+ influx at the plasma membrane. The resulting high Ca2+ levels within the cell seem to directly stimulate PLC activity.

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“…In WEHI 164 fibrosarcoma cells, a methanolic extract of this plant exhibited a significant and dose-dependent reduction of the activity of matrix metalloproteinases ( Hajiaghaee et al, 2007 ), which play a role in tissue remodeling and tumor invasion. An apolar fraction from a methanolic extract of Scrophularia orientalis L. significantly reduced the viability of neuroblastoma cells due to a loss of mitochondrial membrane integrity ( Lange et al, 2016 ). In MCF-7 breast cancer cells, weak cytotoxicity of an ethanolic extract from S. variegata was reported ( Azadmehr et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Scrophularia lucida and the Effects on Tumor Invasiveness in Vitro

et al. 2018

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A detannified methanolic extract of Scrophularia lucida L. attenuated the formation of cancer cell-induced circular chemorepellent induced defects (CCIDs) in the lymph endothelial cell barrier, which resemble entry ports for the intravasating tumor into the vasculature as a prerequisite for lymph node metastasis. Therefore, the composition of this extract was studied in an activity-guided approach. Since no data on the secondary metabolites of this plant were available, first phytochemical data were collected in the course of the fractionation of the extract. The study resulted in the identification of 14 substances, among them very rare iridoids, such as scrovalentinoside or koelzioside, and several flavonoids (e.g., nepitrin and homoplantaginin). One of the latter group, 2″-O-acetyl-homoplantaginin, is a new natural compound. In the most active fraction, the flavonoid hispidulin was identified as major component and the assay of the pure compound confirmed a contribution of hispidulin to the CCID-inhibitory effects of S. lucida. The activity of the two major iridoids in this assay was less compared to hispidulin.

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Scrophularia orientalis extract induces calcium signaling and apoptosis in neuroblastoma cells

Cited by 11 publications

References 45 publications

Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro

Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro

Dandelion Root Extract Induces Intracellular Ca2+ Increases in HEK293 Cells

Chemical Composition of Scrophularia lucida and the Effects on Tumor Invasiveness in Vitro

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