Screening wheat genotypes for resistance to black point and the effects of diseased kernels on seed germination

Li, Qiaoyun; Zhao, Qin; Jiang, Yumei; Shen, Chuncai; Duan, Zongbiao; Niu, Jishan

doi:10.1007/bf03356495

Cited by 32 publications

(44 citation statements)

References 18 publications

(28 reference statements)

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“…The T, M and D in progeny of dms were selected by us on experimental field issued by Henan Agriculture University, Zhengzhou, Henan, China and conserved in our laboratory ( Fig 1 ). The other traits such as tiller number, internode number, floret structure and fertility of both the semi-dwarf and tall plants were almost the same as that of ‘Zhoumai 18’, However, the dwarf plants (D) were significantly different from ‘Zhoumai 18’ and T plants, The average plant height, tiller number, and spike number per plant of D were only 0.29 m, 3.9 and 2.4, respectively, they decreased by 62.4, 80.5 and 84.6% compared with those of ‘Zhoumai 18’ and their spikes were sterile ( Table 1 ) [ 25 ]. Individual plant lines of mutant dms were sown from 2009–2011.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms

Zhu

Shen

et al. 2016

PLoS ONE

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BackgroundWheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype.ResultsFour unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T.ConclusionsWe identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study.

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Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms

Zhu

Shen

et al. 2016

PLoS ONE

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“…The distance between rows was 0.25 m, and 30 seeds were planted in each row. Fertilizer and weed management was similar to that of wheat breeding [83].…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Zhang

et al. 2019

IJMS

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Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.Plant male sterility was first reported in the 18th century [8]. A large number of plant male sterility mutants have been reported over the past few decades. Male sterility includes cytoplasmic male sterility (CMS) controlled by mitochondrial genes coupled with nuclear genes, and genic male sterility (GMS) controlled by nuclear genes alone [9][10][11]. The pistils of the cytoplasmic male sterile lines and genic male sterile lines are normal, but their stamens are abnormal and cannot pollinate normally, which is due to stamen degeneration, pollen abortion, or functional sterility. GMS occurs in 216 species and 17 species crosses, while CMS occurs in more than 150 species and 271 species crosses [11][12][13]. Plant male sterility lines have been widely studied and applied successfully in many crops, such as maize (Zea mays), rice (Oryza sativa), soybean (Glycine max), and barley (Hordeum vulgare). Several wheat CMS lines have been studied and applied in production; however, commercial application is limited [14][15][16]. Possible applications of GMS in plant breeding have been reviewed and discussed for at least 30 years [17]. Up to now, only five GMS genes have been...

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“…Therefore, AK58 was used as susceptible control. Fertilizer and weed management were similar to methods used in the process for wheat breeding (Li et al, 2014). The spikelets and lodicules of YM18 and ZK001, which had three biological replicates, were harvested during the white anther stage (WAS), green anther stage (GAS), yellow anther stage (YAS), and anthesis stage (AS) (Zadoks, Chang & Konzak, 1974;Kirby & Appleyard, 1987;Guo & Schnurbusch, 2015).…”

Section: Plant Materialsmentioning

confidence: 99%

Peer Review #2 of "iTRAQ-based quantitative proteome analysis reveals metabolic changes between a cleistogamous wheat mutant and its wild-type wheat counterpart (v0.2)"

2019

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Background: Wheat is one of the most important staple crops worldwide. Fusarium head blight severely affects wheat yield and quality. A novel bread wheat mutant, ZK001, characterised as cleistogamic was isolated from a non-cleistogamous variety (YM18) through static magnetic field mutagenesis. Cleistogamy is a promising strategy for controlling Fusarium head blight. However, little is known about the mechanism of cleistogamy in wheat. Methods: We performed a Fusarium head blight resistance test to identify the Fusarium head blight infection rate of ZK001. We also measured the agronomic traits of ZK001 and the starch and total soluble sugar contents of lodicules in YM18 and ZK001. Finally, we performed comparative studies at the proteome level between YM18 and ZK001 based on the proteomic technique of isobaric tags for relative and absolute quantification. Results: The infection rate of ZK001 was lower than that of its wild type and AK58. The abnormal lodicules of ZK001 lost the ability to push the lemma and palea apart during the flowering stage. Proteome analysis showed that the main differentially abundant proteins were related to carbohydrate metabolism, protein transport, and calcium ion binding. These differentially abundant proteins may work together to regulate cellular homeostasis, osmotic pressure and the development of lodicules. This hypothesis is supported by the analysis of starch, soluble sugar content in the lodicules as well as the results of qRT-PCR. Conclusions: Proteomic analysis has provided comprehensive information that should be useful for further research on the lodicule development mechanism in wheat. The ZK001 mutant is optimal for studying flower development in wheat and could be very important for Fusarium head blight resistant projects via conventional crossing.

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Screening wheat genotypes for resistance to black point and the effects of diseased kernels on seed germination

Cited by 32 publications

References 18 publications

Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms

Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Peer Review #2 of "iTRAQ-based quantitative proteome analysis reveals metabolic changes between a cleistogamous wheat mutant and its wild-type wheat counterpart (v0.2)"

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