Screening tolerance to phosphorus starvation and haplotype analysis using phosphorus uptake 1 (Pup1) QTL linked markers in rice genotypes

Abstract

Phosphorus (P) deficiency tolerance is a pivotal trait for plant growth and development. Most of the commercial modern cultivars lack this trait and reported it as a very serious problem limiting crop productivity. This trait is advantageous if present in modern high yielding varieties as it increases the yield under the phosphorus-deficient soil conditions. With the importance of phosphorus deficiency tolerance, the present investigation was carried out with an objective to screen for tolerance to phosphorus deficiency using solution culture and phosphorus uptake 1 (Pup1) locus linked markers in 30 diverse rice genotypes. A wide range of varied responses to P deficiency in rice genotypes for all the traits were observed. Root length and enzyme activity showed increased mean performance under the -P condition when compared to ? P condition. Medium to high heritability estimates were obtained for most of the traits. Correlation analysis showed that the traits: root P content, fresh shoot weight, dry shoot weight, and shoot length showed highly significant correlations with each other under -P conditions. Based on the hydroponics and molecular screening, three genotypes viz., ADT (R) 48, Improved Pusa Basmati 1 and UPLRI 5 were classified as tolerant for its response to P deficiency as they possessed significant increase in desirable root and shoot traits, increased acid phosphatase enzyme and these genotypes also possessed the Pup1 allele for all the five markers. The selected genotypes may be useful for the exploration of novel genes conferring phosphorus deficiency tolerance and used as donor parents in the breeding programs. Absence of this allele in the rice genotypes viz., drought tolerant (Anna (R) 4) and submergence tolerant (CR 1009 Sub 1) may warrant the development of multiple abiotic stress tolerance cultivars for upland and submergence cropping systems in future rice breeding program.

Multi‐omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis

“…Although elevated APase activity is commonly observed in Pi‐deficient plants, previous studies have noted variations in APase activity across different genotypes within plant species, such as in rapeseed ( Brassica napus ), lentil ( Lens culinaris ), rice, soybean, and pigeonpea (Ganguly et al., 2021; Nirubana et al., 2020; Sidhu et al., 2018; Tian et al., 2003; Zhang et al., 2010). However, the underlying causes of this genotypic variation remain largely unexplored.…”

Examining Phosphorus Use Efficiency Across Different Lettuce (Lactuca sativa L.) Genotypes

“…Soil exploration capacity is directly related to root architecture and distribution, which in turn, have been found to be critical for lettuce to uptake N and water when these two resources were limited (Johnson et al 2000;Macias-González et al 2021). Perhaps, some lettuce genotypes can access some of the P present in the insoluble complexes formed with Ca and Mg (Gruen et al 2014), including calcium phosphate and magnesium phosphate, through the root exudation of organic compounds such as acid phosphatase enzymes, as observed in barley and rice genotypes (Gao et al 2020;Nirubana et al 2020). Furthermore, P solubilization and uptake by plants might be facilitated via symbiotic associations with soil microorganisms such as mycorrhizal fungi and plant-growth promoting bacteria, which were found to improve soil P availability in the rhizosphere of maize (Oliveira et al 2009).…”

Identifying Lettuce Accessions for Efficient Use of Phosphorus in Hydroponics

“…Hydroponic studies have been conducted to understand the effect of P limitation on physiological parameters and growth of maize (Zea mays L.), sorghum [Sorghum bicolor (L.) Moench], potato, rice (Oryza sativa L.), and lettuce (Bera et al 2018;Delaide et al 2016;Islam et al 2019;Lee et al 2021;Neocleous and Savvas 2019;Nirubana et al 2020;Sapkota et al 2019). Nevertheless, further research is needed to identify and characterize lettuce accessions with higher PUE in hydroponics.…”