Screening the variability in oilseed rape resistance to pollen beetle attacks in the field and assessment of biochemical biomarkers

Seimandi‐Corda, Gaëtan; Renaud, D.L.; Escande, Laure; Lariepe, Amandine; Ollivier, Jérôme; Faure, Sébastien; Cortesero, Anne Marie

doi:10.1007/s10340-018-01070-9

Cited by 4 publications

(9 citation statements)

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“…Correlation of metabolome profiles with the beetles' feeding activity revealed seven glucosinolates, several phenylpropanoids, flavonoids and saponin derivatives as potential antifeedants. In contrast to the studies of Hervé, Delourme, et al (2014) and Seimandi‐Corda et al (2019), who both investigated only B. napus cultivars, in our study, nearly all glucosinolates were negatively correlated with beetles' feeding activity. Negative correlations of pollen beetle abundance with total glucosinolate levels in flower buds of B. napus (Giamoustaris & Mithen, 1996) or oviposition behaviour of females with sinalbin content in flower buds of three S. alba cultivars (Hopkins et al, 1998) were reported before, too.…”

Section: Discussioncontrasting

confidence: 99%

“…Targeted metabolome profiling of the flower buds gave hints that primary metabolites such as sugars and amino acids as well as secondary metabolites such as flavonoids might be involved in the differential preference of the beetles. However, there was a high variation in the beetle response and the bud metabolome between years, laboratory and field studies and field sites (Hervé, Delourme, et al, 2014; Seimandi‐Corda et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Chemical defence in Brassicaceae against pollen beetles revealed by metabolomics and flower bud manipulation approaches

Austel

Böttcher

Meiners

2020

Plant Cell & Environment

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Divergence of chemical plant defence mechanisms within the Brassicaceae can be utilized to identify means against specialized pest insects. Using a bioassay‐driven approach, we (a) screened 24 different Brassica napus cultivars, B. napus resyntheses and related brassicaceous species for natural plant resistance against feeding adults of the pollen beetle (Brassicogethes aeneus), (b) tested for gender‐specific feeding resistance, (c) analysed the flower bud metabolomes by a non‐targeted approach and (d) tested single candidate compounds for their antifeedant activity. (a) In no‐choice assays, beetles were allowed to feed on intact plants. Reduced feeding activity was mainly observed on Sinapis alba and Barbarea vulgaris but not on B. napus cultivars. (b) Males fed less and discriminated more in feeding than females. (c) Correlation of the metabolite abundances with the beetles' feeding activity revealed several glucosinolates, phenylpropanoids, flavonoids and saponins as potential antifeedants. (d) These were tested in dual‐bud‐choice assays developed for medium‐throughput compound screening. Application of standard compounds on single oilseed rape flower buds revealed highly deterrent effects of glucobarbarin, oleanolic acid and hederagenin. These results help to understand chemical plant defence in the Brassicaceae and are of key importance for further breeding strategies for insect‐resistant oilseed rape cultivars.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical defence in Brassicaceae against pollen beetles revealed by metabolomics and flower bud manipulation approaches

Austel

Böttcher

Meiners

2020

Plant Cell & Environment

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“…Counting damaged buds could be used to more accurately monitor how pollen beetles are impacting the crop and for determining when treatment is necessary. However, the numbers of damaged buds were affected by growth stage, 42 so growth‐stage specific thresholds may be required. The method could become time‐consuming when large numbers of plants at more advanced growth stages (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Others focused on the number of adult insects sampled by beating plants, 28,32–39 sweep netting 29,32,40 or vacuum suction sampling 41 . Some studies also reported on the damage caused by the pollen beetle and directly assessed the number of buds damaged before flowering 35,42 ; or more commonly, the number of podless stalks after flowering 19,32,43 …”

Section: Introductionmentioning

confidence: 99%

“…30,31 Others focused on the number of adult insects sampled by beating plants, 28,[32][33][34][35][36][37][38][39] sweep netting 29,32,40 or vacuum suction sampling. 41 Some studies also reported on the damage caused by the pollen beetle and directly assessed the number of buds damaged before flowering 35,42 ; or more commonly, the number of podless stalks after flowering. 19,32,43 Methods dealing with insect abundance are commonly used to monitor pollen beetle migration to the field, but they have also been used to estimate insect pressure as they can be easily linked to control thresholds.…”

Section: Introductionmentioning

confidence: 99%

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Sampling pollen beetle (Brassicogethes aeneus) pressure in oilseed rape: which method is best?

Seimandi‐Corda

Jenkins

Cook

2021

Pest Management Science

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BACKGROUND The pollen beetle (Brassicogethes aeneus) is the most abundant pest of oilseed rape in spring and is potentially one of the most damaging. Adults feed on the pollen within closed flower buds and the damage leads to bud abscission, resulting in podless stalks and yield reduction. Several methods are currently used to monitor the pressure of this insect, such as counting the numbers of adults on the plants, quantifying the number of buds damaged by the insect before flowering or counting the number of podless stalks before harvest. We conducted experiments to evaluate the robustness of these sampling methods and compared their results. We also describe how pollen beetles damage the plants to understand the limitations of methods based on damage estimation. RESULTS Methods based on adult abundance lack robustness. We observed that most of the damage to buds is caused by pollen beetles feeding on small buds (< 3 mm), and that this damage can be quantified later in the season, indicating that methods based on the count of podless stalks are robust. Different methods gave consistent results and quantification of the pressure on the primary raceme can be a good proxy for pressure on the whole plant. CONCLUSIONS Standardised methods for assessment of pollen beetle pressure will enable comparison of pest management strategies between different studies and facilitate the development of alternative control strategies for this pest. © 2021 Rothamsted Research. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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A protocol for increased throughput phenotyping of plant resistance to the pollen beetle

Bellec

Hervé

Mercier

et al. 2022

Pest Management Science

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BACKGROUND: Improving crop resistance to insect herbivores is a major research objective in breeding programs. Although genomic technologies have increased the speed at which large populations can be genotyped, breeding programs still suffer from phenotyping constraints. The pollen beetle (Brassicogethes aeneus) is a major pest of oilseed rape for which no resistant cultivar is available to date, but previous studies have highlighted the potential of white mustard as a source of resistance and introgression of this resistance appears to be a promising strategy. Here we present a phenotyping protocol allowing midthroughput (i.e., increased throughput compared to current methods) acquisition of resistance data, which could then be used for genetic mapping of QTLs.RESULTS: Contrasted white mustard genotypes were selected from an initial field screening and then evaluated for their resistance under controlled conditions using a standard phenotyping method on entire plants. We then upgraded this protocol for mid-throughput phenotyping, by testing two alternative methods. We found that phenotyping on detached buds did not provide the same resistance contrasts as observed with the standard protocol, in contrast to the phenotyping protocol with miniaturized plants. This protocol was then tested on a large panel composed of hundreds of plants. A significant variation in resistance among genotypes was observed, which validates the large-scale application of this new phenotyping protocol.CONCLUSION: The combination of this mid-throughput phenotyping protocol and white mustard as a source of resistance against the pollen beetle offers a promising avenue for breeding programs aiming to improve oilseed rape resistance.

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Screening the variability in oilseed rape resistance to pollen beetle attacks in the field and assessment of biochemical biomarkers

Cited by 4 publications

References 65 publications

Chemical defence in Brassicaceae against pollen beetles revealed by metabolomics and flower bud manipulation approaches

Chemical defence in Brassicaceae against pollen beetles revealed by metabolomics and flower bud manipulation approaches

Sampling pollen beetle (Brassicogethes aeneus) pressure in oilseed rape: which method is best?

A protocol for increased throughput phenotyping of plant resistance to the pollen beetle

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