Search for an alternative to chemical herbicides have prompted researchers to investigate the allelopathic potential of plants, which would serve as a remedy for contamination of soil, water bodies and crops products with toxic chemical residues derived from using synthetic herbicides. The allelopathic effects of the aqueous leaf extracts of Ageratum conyzoides, Vernonia amygdalina and Artemisia annua at 0.05, 0.1, 0.2 and 0.5 (part per volume) concentrations were evaluated on seed germination, root length and height of seedlings of Lactuca sativa after 28 days in vitro. Results obtained showed significant differences P=[0.5] among the different concentrations of aqueous leaf extracts used when compared with the control in all the three parameters studied. Significant reduction in seed germination, growth of root and height of seedlings were observed in all the treatments but at varying levels from moderate to severe effects for aqueous leaf extract of A. conyzoides and A. annua respectively, while on the contrary, aqueous extracts of V. amygdalina