Screening the activity of plants and spices for decreasing ruminal methane production in vitro

García‐González, Ricardo; López, Secundino; Valle, Pilar del; Bodas, Raúl; González, Jesús Salvador

doi:10.1016/j.anifeedsci.2007.09.008

Cited by 100 publications

(75 citation statements)

References 35 publications

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“…Atriplex showed the highest value for GP per unit of incubated OM compared with the other browses, but when expressed per unit for degraded OM, Tifton hay had the highest value, which may be due to differences in rumen degradability. García-González et al (2008) observed that GP and CH 4 emissions are closely related to the amount of ruminally fermented OM or the amount of digestible OM. Thus GP and CH 4 are better expressed per unit degraded OM than per incubated OM.…”

Section: Discussionmentioning

confidence: 97%

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Soltan¹,

Morsy²,

Sallam³

et al. 2012

J. Anim. Feed Sci.

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Two experiments in vitro were conducted to evaluate four Egyptian forage legume browses, i.e., leaves of prosopis (Prosopis juliflora), acacia (Acacia saligna), atriplex (Atriplex halimus), and leucaena (Leucaena leucocephala), in comparison with Tifton (Cynodon sp.) grass hay for their gas production, methanogenic potential, and ruminal fermentation using a semi-automatic system for gas production (first experiment) and for ruminal and post ruminal protein degradability (second experiment). Acacia and leucaena showed pronounced methane inhibition compared with Tifton, while prosopis and leucaena decreased the acetate:propionate ratio (P<0.01). Acacia and leucaena presented a lower (P<0.01) ruminal NH 3 -N concentration associated with the decreasing (P<0.01) ruminal protein degradability. Leucaena, however, showed higher (P<0.01) intestinal protein digestibility than acacia. This study suggests that the potential methanogenic properties of leguminous browses may be related not only to tannin content, but also to other factors.

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Section: Discussionmentioning

confidence: 97%

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Soltan¹,

Morsy²,

Sallam³

et al. 2012

J. Anim. Feed Sci.

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“…Such experimental approaches have allowed the mode of action of a range of chemicals (Busquet et al, 2005;Bodas et al, 2008;García-González et al, 2008;Durmic et al, 2010) and dietary substrates (Patra and Yu, 2013;Hatew et al, 2015) to be investigated. Use of IVFT offers the opportunity to evaluate a broad spectrum of chemical agents alone or in a number of combinations over a wide range of concentrations (e.g., Busquet et al, 2005;García-González et al, 2008). However, this technique does not generate reliable information for agents that are only effective for decreasing CH 4 emissions over an extended period (Castro-Montoya et al, 2015).…”

Section: Tablementioning

confidence: 99%

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Yáñez-Ruíz

Bannink

Dijkstra

et al. 2016

Animal Feed Science and Technology

130

120

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a b s t r a c tIn vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH 4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH 4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH 4 production.

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“…These two properties were hypothesized to affect those rumen microbes that are involved in ruminal lipid metabolism and might explain the characteristic fatty acid profile of alpine milk (Leiber et al, 2005). Some plant species found in alpine habitats are additionally known for their anti-microbial properties (García-Gonzá lez et al, 2008). Therefore, alpine pasture grass can be expected to modify ruminal fermentation in general, which could also affect methanogenesis.…”

Section: Introductionmentioning

confidence: 99%

Effects of species-diverse high-alpine forage on in vitro ruminal fermentation when used as donor cow's feed or directly incubated

Khiaosa‐ard

Soliva

Kreuzer

et al. 2012

Animal

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Alpine forages are assumed to have specific effects on ruminal digestion when fed to cattle. These effects were investigated in an experiment from two perspectives, either by using such forages as a substrate for incubation or as feed for a rumen fluid donor cow. In total, six 24-hin vitrobatch culture runs were performed. Rumen fluid was collected from a non-lactating donor cow after having grazed pastures at ∼2000 m above sea level for 2, 6 and 10 weeks. These ‘alpine runs’ were compared with three lowland samplings from before and 2 and 6 weeks after the alpine grazing where a silage–concentrate mix was fed. In each run, nine replicates of four forages each were incubated. These forages differed in type and origin (alpine hay, lowland ryegrass hay, grass–maize silage mix, pure hemicellulose) as well as in the content of nutrients. Concentrations of phenolic compounds in the incubated forages were (g/kg dry matter (DM)): 20 (tannin proportion: 0.47), 8 (0.27), 15 (0.52) and 0 (0), respectively. Crude protein was highest in the silage mix and lowest with hemicellulose, whereas the opposite was the case for fiber. The total phenol contents (g/kg DM) for the high altitude and the lowland diet of the donor cow were 27 (tannins: 0.50 of phenols) and 12 (0.27), respectively. Independent of the origin of the rumen fluid, the incubation with alpine hay decreased (P< 0.05) bacterial counts, fermentation gas amount, volatile fatty acid (VFA) production as well as ammonia and methane concentrations in fermentation gas (the latter two being not lower when compared with hemicellulose). Alpine grazing of the cow in turn increased (P< 0.001) bacterial counts and, to a lesser extent, acetate proportion compared with lowland feeding. Further, alpine grazing decreased protozoal count (P< 0.05) and VFA production (P< 0.001) to a small extent, whereas methane remained widely unchanged. There were interactions (P< 0.05) between forage type incubated and feeding period of the donor cow in protozoal counts, acetate:propionate ratio, fermentation gas production and its content of methane,in vitroorganic matter digestibility and metabolizable energy. Although increased phenolic compounds were the most consistent common property of the applied alpine forages, a clear attribution to certain effects was not possible in this study. As a further result, adaptation (long-term for donor cow, short term for 24 h incubations) appears to influence the expression of alpine forage effects in ruminal fermentation.

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Screening the activity of plants and spices for decreasing ruminal methane production in vitro

Cited by 100 publications

References 35 publications

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Comparative <i>in vitro</i> evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis

Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants—a review

Effects of species-diverse high-alpine forage on in vitro ruminal fermentation when used as donor cow's feed or directly incubated

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